Experiment: LB + carbenicillin regrowth after 48hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt motA and flhC are separated by 126 nucleotides flhC and flhD are separated by 2 nucleotides flhD and insB-5 are separated by 320 nucleotides insB-5 and insA-5 overlap by 82 nucleotides
b1890: motA - flagellar motor protein MotA (NCBI), at 1,974,276 to 1,975,163
motA
b1891: flhC - DNA-binding transcriptional dual regulator with FlhD (NCBI), at 1,975,290 to 1,975,868
flhC
b1892: flhD - DNA-binding transcriptional dual regulator with FlhC (RefSeq), at 1,975,871 to 1,976,221
flhD
b1893: insB-5 - IS1 transposase InsAB' (NCBI), at 1,976,542 to 1,977,045
insB-5
b1894: insA-5 - IS1 repressor protein InsA (NCBI), at 1,976,964 to 1,977,239
insA-5
Position (kb)
1975
1976
1977 Strain fitness (log2 ratio)
-1
0
1 at 1974.934 kb on + strand, within motA at 1974.934 kb on + strand, within motA at 1975.040 kb on + strand, within motA at 1975.092 kb on + strand at 1975.093 kb on - strand at 1975.173 kb on + strand at 1975.179 kb on + strand at 1975.179 kb on + strand at 1975.415 kb on - strand, within flhC at 1975.477 kb on + strand, within flhC at 1975.589 kb on + strand, within flhC at 1975.636 kb on + strand, within flhC at 1975.707 kb on + strand, within flhC at 1975.914 kb on - strand, within flhD at 1975.961 kb on - strand, within flhD at 1975.961 kb on - strand, within flhD at 1975.991 kb on + strand, within flhD at 1975.991 kb on - strand, within flhD at 1976.072 kb on + strand, within flhD at 1976.072 kb on + strand, within flhD at 1976.072 kb on + strand, within flhD at 1976.120 kb on + strand, within flhD at 1976.120 kb on + strand, within flhD at 1976.138 kb on + strand, within flhD at 1976.146 kb on - strand, within flhD at 1976.149 kb on - strand, within flhD at 1976.149 kb on - strand, within flhD at 1976.221 kb on - strand at 1976.226 kb on - strand at 1976.226 kb on - strand at 1976.259 kb on + strand at 1976.262 kb on + strand at 1976.272 kb on + strand at 1976.281 kb on + strand at 1976.354 kb on - strand at 1976.387 kb on + strand at 1976.396 kb on - strand at 1976.432 kb on - strand at 1976.436 kb on + strand at 1976.439 kb on + strand at 1976.439 kb on + strand at 1976.439 kb on + strand at 1976.439 kb on + strand at 1976.444 kb on + strand at 1976.447 kb on - strand at 1976.447 kb on - strand at 1976.447 kb on - strand at 1976.447 kb on - strand at 1976.448 kb on + strand at 1976.452 kb on - strand at 1976.452 kb on - strand at 1976.453 kb on + strand at 1976.454 kb on - strand at 1976.455 kb on - strand at 1976.460 kb on - strand at 1976.514 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB + carbenicillin regrowth after 48hr remove 1,974,934 + motA b1890 0.74 -0.0 1,974,934 + motA b1890 0.74 +0.3 1,975,040 + motA b1890 0.86 -1.0 1,975,092 + +0.0 1,975,093 - +0.3 1,975,173 + +0.3 1,975,179 + +0.2 1,975,179 + +0.2 1,975,415 - flhC b1891 0.22 +0.0 1,975,477 + flhC b1891 0.32 -1.0 1,975,589 + flhC b1891 0.52 +0.3 1,975,636 + flhC b1891 0.60 +0.5 1,975,707 + flhC b1891 0.72 +0.2 1,975,914 - flhD b1892 0.12 +1.0 1,975,961 - flhD b1892 0.26 +0.7 1,975,961 - flhD b1892 0.26 +0.1 1,975,991 + flhD b1892 0.34 +0.3 1,975,991 - flhD b1892 0.34 +0.1 1,976,072 + flhD b1892 0.57 -0.8 1,976,072 + flhD b1892 0.57 +0.8 1,976,072 + flhD b1892 0.57 +0.2 1,976,120 + flhD b1892 0.71 +1.3 1,976,120 + flhD b1892 0.71 +0.5 1,976,138 + flhD b1892 0.76 +0.5 1,976,146 - flhD b1892 0.78 +0.5 1,976,149 - flhD b1892 0.79 +0.7 1,976,149 - flhD b1892 0.79 -0.0 1,976,221 - -0.6 1,976,226 - +1.6 1,976,226 - +0.6 1,976,259 + +0.3 1,976,262 + +0.3 1,976,272 + -0.1 1,976,281 + -0.3 1,976,354 - -0.2 1,976,387 + +0.1 1,976,396 - +0.2 1,976,432 - -1.6 1,976,436 + +0.5 1,976,439 + +0.6 1,976,439 + -0.1 1,976,439 + -0.3 1,976,439 + +0.3 1,976,444 + +0.2 1,976,447 - +0.9 1,976,447 - -0.2 1,976,447 - -0.6 1,976,447 - +1.4 1,976,448 + +0.3 1,976,452 - +0.2 1,976,452 - -1.0 1,976,453 + -0.1 1,976,454 - -0.7 1,976,455 - -0.0 1,976,460 - -0.4 1,976,514 - -0.1
Or see this region's nucleotide sequence