Strain Fitness in Escherichia coli BW25113 around b1462

Experiment: LB + carbenicillin regrowth after 48hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydcC and ydcE are separated by 99 nucleotidesydcE and b1462 overlap by 4 nucleotidesb1462 and nhoA are separated by 172 nucleotides b1460: ydcC - hypothetical protein (NCBI), at 1,529,840 to 1,530,976 ydcC b1461: ydcE - 4-oxalocrotonate tautomerase (NCBI), at 1,531,076 to 1,531,309 ydcE b1462: b1462 - orf, hypothetical protein (VIMSS), at 1,531,306 to 1,531,875 b1462 b1463: nhoA - N-hydroxyarylamine O-acetyltransferase (NCBI), at 1,532,048 to 1,532,893 nhoA Position (kb) 1531 1532Strain fitness (log2 ratio) -1 0 1 2at 1531.010 kb on + strandat 1531.025 kb on + strandat 1531.035 kb on - strandat 1531.066 kb on - strandat 1531.128 kb on + strand, within ydcEat 1531.128 kb on + strand, within ydcEat 1531.177 kb on + strand, within ydcEat 1531.197 kb on + strand, within ydcEat 1531.197 kb on + strand, within ydcEat 1531.210 kb on - strand, within ydcEat 1531.211 kb on - strand, within ydcEat 1531.215 kb on - strand, within ydcEat 1531.233 kb on - strand, within ydcEat 1531.238 kb on + strand, within ydcEat 1531.238 kb on + strandat 1531.381 kb on - strand, within b1462at 1531.599 kb on + strand, within b1462at 1531.972 kb on - strandat 1531.972 kb on - strandat 1532.030 kb on - strandat 1532.030 kb on - strandat 1532.151 kb on - strand, within nhoAat 1532.151 kb on - strand, within nhoAat 1532.151 kb on - strand, within nhoAat 1532.159 kb on + strand, within nhoAat 1532.159 kb on + strand, within nhoAat 1532.497 kb on - strand, within nhoAat 1532.497 kb on - strand, within nhoAat 1532.501 kb on + strand, within nhoAat 1532.501 kb on + strand, within nhoAat 1532.517 kb on - strand, within nhoAat 1532.530 kb on - strand, within nhoAat 1532.595 kb on + strand, within nhoAat 1532.688 kb on + strand, within nhoAat 1532.696 kb on - strand, within nhoAat 1532.746 kb on + strand, within nhoAat 1532.785 kb on - strand, within nhoAat 1532.834 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin regrowth after 48hr
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1,531,010 + +1.4
1,531,025 + +0.8
1,531,035 - +0.9
1,531,066 - +0.1
1,531,128 + ydcE b1461 0.22 -0.6
1,531,128 + ydcE b1461 0.22 +0.7
1,531,177 + ydcE b1461 0.43 +2.4
1,531,197 + ydcE b1461 0.52 +0.4
1,531,197 + ydcE b1461 0.52 +0.4
1,531,210 - ydcE b1461 0.57 +0.3
1,531,211 - ydcE b1461 0.58 +0.4
1,531,215 - ydcE b1461 0.59 +0.3
1,531,233 - ydcE b1461 0.67 -0.0
1,531,238 + ydcE b1461 0.69 -0.0
1,531,238 + +0.5
1,531,381 - b1462 b1462 0.13 +0.5
1,531,599 + b1462 b1462 0.51 +0.2
1,531,972 - +0.7
1,531,972 - +1.0
1,532,030 - +0.2
1,532,030 - +0.3
1,532,151 - nhoA b1463 0.12 +0.3
1,532,151 - nhoA b1463 0.12 +0.6
1,532,151 - nhoA b1463 0.12 -0.2
1,532,159 + nhoA b1463 0.13 +0.2
1,532,159 + nhoA b1463 0.13 +0.2
1,532,497 - nhoA b1463 0.53 +0.1
1,532,497 - nhoA b1463 0.53 -0.0
1,532,501 + nhoA b1463 0.54 +0.5
1,532,501 + nhoA b1463 0.54 +0.8
1,532,517 - nhoA b1463 0.55 +0.8
1,532,530 - nhoA b1463 0.57 +0.0
1,532,595 + nhoA b1463 0.65 +0.5
1,532,688 + nhoA b1463 0.76 -0.1
1,532,696 - nhoA b1463 0.77 +0.1
1,532,746 + nhoA b1463 0.83 +0.2
1,532,785 - nhoA b1463 0.87 +0.8
1,532,834 - +0.0

Or see this region's nucleotide sequence