Strain Fitness in Escherichia coli BW25113 around b1081

Experiment: LB + carbenicillin regrowth after 48hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntflgI and flgJ overlap by 1 nucleotidesflgJ and flgK are separated by 65 nucleotides b1080: flgI - homolog of Salmonella P-ring of flagella basal body (VIMSS), at 1,135,497 to 1,136,594 flgI b1081: flgJ - flagellar biosynthesis protein FlgJ (NCBI), at 1,136,594 to 1,137,535 flgJ b1082: flgK - flagellar hook-associated protein K (NCBI), at 1,137,601 to 1,139,244 flgK Position (kb) 1136 1137 1138Strain fitness (log2 ratio) -1 0 1 2at 1135.634 kb on - strand, within flgIat 1135.838 kb on + strand, within flgIat 1135.849 kb on + strand, within flgIat 1135.867 kb on - strand, within flgIat 1135.913 kb on - strand, within flgIat 1136.103 kb on + strand, within flgIat 1136.129 kb on - strand, within flgIat 1136.129 kb on - strand, within flgIat 1136.165 kb on - strand, within flgIat 1136.168 kb on + strand, within flgIat 1136.312 kb on - strand, within flgIat 1136.312 kb on - strand, within flgIat 1136.319 kb on + strand, within flgIat 1136.335 kb on - strand, within flgIat 1136.399 kb on + strand, within flgIat 1136.413 kb on - strand, within flgIat 1136.451 kb on + strand, within flgIat 1136.507 kb on + strandat 1136.507 kb on + strandat 1136.620 kb on - strandat 1136.622 kb on - strandat 1136.622 kb on - strandat 1136.776 kb on + strand, within flgJat 1136.776 kb on + strand, within flgJat 1136.810 kb on + strand, within flgJat 1136.810 kb on + strand, within flgJat 1136.821 kb on + strand, within flgJat 1136.854 kb on - strand, within flgJat 1136.867 kb on - strand, within flgJat 1136.957 kb on - strand, within flgJat 1137.015 kb on - strand, within flgJat 1137.015 kb on - strand, within flgJat 1137.122 kb on - strand, within flgJat 1137.373 kb on - strand, within flgJat 1137.452 kb on + strandat 1137.684 kb on + strandat 1137.691 kb on - strandat 1137.691 kb on - strandat 1137.705 kb on - strandat 1137.740 kb on - strandat 1138.112 kb on - strand, within flgKat 1138.255 kb on + strand, within flgKat 1138.306 kb on + strand, within flgKat 1138.350 kb on + strand, within flgKat 1138.362 kb on + strand, within flgKat 1138.369 kb on + strand, within flgKat 1138.422 kb on + strand, within flgKat 1138.422 kb on + strand, within flgKat 1138.469 kb on + strand, within flgKat 1138.530 kb on - strand, within flgK

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin regrowth after 48hr
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1,135,634 - flgI b1080 0.12 -0.4
1,135,838 + flgI b1080 0.31 +0.1
1,135,849 + flgI b1080 0.32 -0.2
1,135,867 - flgI b1080 0.34 +0.4
1,135,913 - flgI b1080 0.38 +0.2
1,136,103 + flgI b1080 0.55 +0.2
1,136,129 - flgI b1080 0.58 +0.9
1,136,129 - flgI b1080 0.58 +0.9
1,136,165 - flgI b1080 0.61 +0.4
1,136,168 + flgI b1080 0.61 +0.3
1,136,312 - flgI b1080 0.74 +0.1
1,136,312 - flgI b1080 0.74 +0.4
1,136,319 + flgI b1080 0.75 +0.1
1,136,335 - flgI b1080 0.76 -0.4
1,136,399 + flgI b1080 0.82 +0.3
1,136,413 - flgI b1080 0.83 -0.0
1,136,451 + flgI b1080 0.87 -0.1
1,136,507 + +0.5
1,136,507 + +0.3
1,136,620 - +1.8
1,136,622 - +0.1
1,136,622 - +0.4
1,136,776 + flgJ b1081 0.19 +0.4
1,136,776 + flgJ b1081 0.19 +0.4
1,136,810 + flgJ b1081 0.23 +0.5
1,136,810 + flgJ b1081 0.23 +0.4
1,136,821 + flgJ b1081 0.24 +0.3
1,136,854 - flgJ b1081 0.28 +0.5
1,136,867 - flgJ b1081 0.29 +0.6
1,136,957 - flgJ b1081 0.39 +0.3
1,137,015 - flgJ b1081 0.45 +1.8
1,137,015 - flgJ b1081 0.45 +0.4
1,137,122 - flgJ b1081 0.56 +0.0
1,137,373 - flgJ b1081 0.83 +0.2
1,137,452 + +0.2
1,137,684 + +0.1
1,137,691 - +0.7
1,137,691 - +0.2
1,137,705 - +0.2
1,137,740 - +0.7
1,138,112 - flgK b1082 0.31 -0.1
1,138,255 + flgK b1082 0.40 +0.3
1,138,306 + flgK b1082 0.43 +0.1
1,138,350 + flgK b1082 0.46 +0.6
1,138,362 + flgK b1082 0.46 +0.1
1,138,369 + flgK b1082 0.47 -0.0
1,138,422 + flgK b1082 0.50 -0.1
1,138,422 + flgK b1082 0.50 -0.2
1,138,469 + flgK b1082 0.53 +0.4
1,138,530 - flgK b1082 0.57 +0.1

Or see this region's nucleotide sequence