Strain Fitness in Escherichia coli BW25113 around b1000

Experiment: LB + carbenicillin regrowth after 48hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttorD and cbpM are separated by 151 nucleotidescbpM and cbpA overlap by 1 nucleotidescbpA and yccE are separated by 260 nucleotides b0998: torD - chaperone protein TorD (NCBI), at 1,061,022 to 1,061,621 torD b0999: cbpM - modulator of CbpA co-chaperone (NCBI), at 1,061,773 to 1,062,078 cbpM b1000: cbpA - curved DNA-binding protein, DnaJ homologue that functions as a co-chaperone of DnaK (NCBI), at 1,062,078 to 1,062,998 cbpA b1001: yccE - hypothetical protein (NCBI), at 1,063,259 to 1,064,515 yccE Position (kb) 1062 1063Strain fitness (log2 ratio) -1 0 1at 1061.083 kb on - strand, within torDat 1061.747 kb on - strandat 1061.789 kb on - strandat 1061.879 kb on - strand, within cbpMat 1061.889 kb on - strand, within cbpMat 1061.978 kb on - strand, within cbpMat 1061.978 kb on - strand, within cbpMat 1062.190 kb on + strand, within cbpAat 1062.191 kb on - strand, within cbpAat 1062.191 kb on - strand, within cbpAat 1062.401 kb on - strand, within cbpAat 1062.545 kb on - strand, within cbpAat 1062.572 kb on + strand, within cbpAat 1062.672 kb on + strand, within cbpAat 1062.672 kb on + strand, within cbpAat 1062.809 kb on - strand, within cbpAat 1062.917 kb on + strandat 1062.939 kb on - strandat 1063.052 kb on + strandat 1063.052 kb on + strandat 1063.059 kb on + strandat 1063.059 kb on + strandat 1063.059 kb on + strandat 1063.059 kb on + strandat 1063.059 kb on + strandat 1063.067 kb on - strandat 1063.067 kb on - strandat 1063.067 kb on - strandat 1063.067 kb on - strandat 1063.067 kb on - strandat 1063.067 kb on - strandat 1063.067 kb on - strandat 1063.122 kb on + strandat 1063.130 kb on - strandat 1063.135 kb on - strandat 1063.148 kb on - strandat 1063.207 kb on + strandat 1063.207 kb on + strandat 1063.207 kb on + strandat 1063.207 kb on + strandat 1063.207 kb on + strandat 1063.312 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin regrowth after 48hr
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1,061,083 - torD b0998 0.10 +0.4
1,061,747 - +0.1
1,061,789 - +0.5
1,061,879 - cbpM b0999 0.35 +0.2
1,061,889 - cbpM b0999 0.38 -0.3
1,061,978 - cbpM b0999 0.67 -0.1
1,061,978 - cbpM b0999 0.67 +0.1
1,062,190 + cbpA b1000 0.12 +0.3
1,062,191 - cbpA b1000 0.12 +0.2
1,062,191 - cbpA b1000 0.12 +0.8
1,062,401 - cbpA b1000 0.35 +0.4
1,062,545 - cbpA b1000 0.51 +0.5
1,062,572 + cbpA b1000 0.54 +0.6
1,062,672 + cbpA b1000 0.64 -0.5
1,062,672 + cbpA b1000 0.64 -0.2
1,062,809 - cbpA b1000 0.79 +0.7
1,062,917 + -0.3
1,062,939 - +0.1
1,063,052 + +0.2
1,063,052 + +0.4
1,063,059 + +0.2
1,063,059 + +0.5
1,063,059 + +0.5
1,063,059 + +0.3
1,063,059 + +0.6
1,063,067 - +1.6
1,063,067 - +0.6
1,063,067 - +0.1
1,063,067 - -0.2
1,063,067 - +0.4
1,063,067 - +0.3
1,063,067 - +0.3
1,063,122 + -0.0
1,063,130 - +1.0
1,063,135 - +0.8
1,063,148 - +0.2
1,063,207 + -0.2
1,063,207 + +0.3
1,063,207 + +0.3
1,063,207 + -0.4
1,063,207 + -0.4
1,063,312 + +0.4

Or see this region's nucleotide sequence