Experiment: LB + carbenicillin regrowth after 48hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yaeJ and nlpE are separated by 13 nucleotides nlpE and yaeF are separated by 199 nucleotides yaeF and proS are separated by 53 nucleotides
b0191: yaeJ - hypothetical protein (NCBI), at 214,833 to 215,255
yaeJ
b0192: nlpE - lipoprotein involved with copper homeostasis and adhesion (NCBI), at 215,269 to 215,979
nlpE
b0193: yaeF - orf, hypothetical protein (VIMSS), at 216,179 to 217,003
yaeF
b0194: proS - prolyl-tRNA synthetase (NCBI), at 217,057 to 218,775
proS
Position (kb) 216
217
218 Strain fitness (log2 ratio) -2
-1
0
1 at 215.299 kb on + strand at 215.372 kb on + strand, within nlpE at 215.378 kb on + strand, within nlpE at 215.410 kb on + strand, within nlpE at 215.476 kb on - strand, within nlpE at 215.498 kb on - strand, within nlpE at 215.498 kb on - strand, within nlpE at 215.558 kb on + strand, within nlpE at 215.582 kb on - strand, within nlpE at 215.621 kb on + strand, within nlpE at 215.635 kb on - strand, within nlpE at 215.635 kb on - strand, within nlpE at 215.694 kb on + strand, within nlpE at 215.756 kb on + strand, within nlpE at 215.816 kb on + strand, within nlpE at 215.816 kb on + strand, within nlpE at 215.824 kb on - strand, within nlpE at 215.864 kb on - strand, within nlpE at 215.864 kb on - strand, within nlpE at 215.895 kb on - strand, within nlpE at 215.895 kb on - strand, within nlpE at 215.897 kb on - strand, within nlpE at 215.915 kb on - strand at 215.915 kb on - strand at 215.931 kb on - strand at 215.931 kb on - strand at 215.933 kb on - strand at 215.933 kb on - strand at 215.958 kb on + strand at 215.968 kb on - strand at 216.101 kb on - strand at 216.101 kb on - strand at 216.101 kb on - strand at 216.151 kb on + strand at 216.159 kb on - strand at 216.159 kb on - strand at 216.160 kb on - strand at 216.160 kb on - strand at 216.235 kb on + strand at 216.235 kb on + strand at 216.282 kb on + strand, within yaeF at 216.285 kb on + strand, within yaeF at 216.285 kb on + strand, within yaeF at 216.326 kb on - strand, within yaeF at 216.332 kb on - strand, within yaeF at 216.332 kb on - strand, within yaeF at 216.334 kb on + strand, within yaeF at 216.334 kb on + strand, within yaeF at 216.342 kb on - strand, within yaeF at 216.342 kb on - strand, within yaeF at 216.417 kb on + strand, within yaeF at 216.417 kb on - strand, within yaeF at 216.417 kb on - strand, within yaeF at 216.444 kb on + strand, within yaeF at 216.505 kb on + strand, within yaeF at 216.635 kb on + strand, within yaeF at 216.733 kb on + strand, within yaeF at 216.749 kb on - strand, within yaeF at 216.774 kb on - strand, within yaeF at 216.786 kb on - strand, within yaeF at 216.804 kb on + strand, within yaeF at 216.868 kb on - strand, within yaeF at 216.896 kb on + strand, within yaeF at 216.896 kb on + strand, within yaeF at 217.010 kb on - strand at 217.354 kb on + strand, within proS
Per-strain Table
Position Strand Gene LocusTag Fraction LB + carbenicillin regrowth after 48hr remove 215,299 + +0.8 215,372 + nlpE b0192 0.14 +0.3 215,378 + nlpE b0192 0.15 +0.2 215,410 + nlpE b0192 0.20 -0.1 215,476 - nlpE b0192 0.29 +0.0 215,498 - nlpE b0192 0.32 +1.2 215,498 - nlpE b0192 0.32 +0.2 215,558 + nlpE b0192 0.41 +0.0 215,582 - nlpE b0192 0.44 -0.1 215,621 + nlpE b0192 0.50 +0.2 215,635 - nlpE b0192 0.51 +1.4 215,635 - nlpE b0192 0.51 -0.3 215,694 + nlpE b0192 0.60 +0.3 215,756 + nlpE b0192 0.68 +1.4 215,816 + nlpE b0192 0.77 +0.9 215,816 + nlpE b0192 0.77 -0.0 215,824 - nlpE b0192 0.78 -0.1 215,864 - nlpE b0192 0.84 +1.0 215,864 - nlpE b0192 0.84 -0.1 215,895 - nlpE b0192 0.88 +1.3 215,895 - nlpE b0192 0.88 +0.4 215,897 - nlpE b0192 0.88 +0.3 215,915 - +0.4 215,915 - +0.3 215,931 - +0.0 215,931 - +0.4 215,933 - +0.2 215,933 - +0.0 215,958 + -0.2 215,968 - +0.4 216,101 - +0.1 216,101 - +0.0 216,101 - -0.2 216,151 + -0.1 216,159 - +0.3 216,159 - +0.6 216,160 - +0.3 216,160 - +0.3 216,235 + +0.1 216,235 + +0.1 216,282 + yaeF b0193 0.12 +0.2 216,285 + yaeF b0193 0.13 +0.5 216,285 + yaeF b0193 0.13 +0.2 216,326 - yaeF b0193 0.18 +0.8 216,332 - yaeF b0193 0.19 +0.1 216,332 - yaeF b0193 0.19 -0.4 216,334 + yaeF b0193 0.19 +0.1 216,334 + yaeF b0193 0.19 +0.3 216,342 - yaeF b0193 0.20 +0.5 216,342 - yaeF b0193 0.20 +0.3 216,417 + yaeF b0193 0.29 +0.7 216,417 - yaeF b0193 0.29 +1.2 216,417 - yaeF b0193 0.29 +1.2 216,444 + yaeF b0193 0.32 +0.2 216,505 + yaeF b0193 0.40 +0.8 216,635 + yaeF b0193 0.55 +0.8 216,733 + yaeF b0193 0.67 +0.4 216,749 - yaeF b0193 0.69 +0.3 216,774 - yaeF b0193 0.72 -0.0 216,786 - yaeF b0193 0.74 +0.2 216,804 + yaeF b0193 0.76 +0.2 216,868 - yaeF b0193 0.84 +0.5 216,896 + yaeF b0193 0.87 -0.2 216,896 + yaeF b0193 0.87 +0.5 217,010 - +0.4 217,354 + proS b0194 0.17 -2.0
Or see this region's nucleotide sequence