Experiment: LB + carbenicillin regrowth after 48hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt accA and ldcC are separated by 98 nucleotides ldcC and yaeR are separated by 56 nucleotides yaeR and tilS are separated by 64 nucleotides
b0185: accA - acetyl-CoA carboxylase subunit alpha (NCBI), at 208,621 to 209,580
accA
b0186: ldcC - lysine decarboxylase 2, constitutive (NCBI), at 209,679 to 211,820
ldcC
b0187: yaeR - orf, hypothetical protein (VIMSS), at 211,877 to 212,266
yaeR
b0188: tilS - tRNA(Ile)-lysidine synthetase (NCBI), at 212,331 to 213,629
tilS
Position (kb) 209
210
211
212 Strain fitness (log2 ratio) -1
0
1 at 209.655 kb on - strand at 209.826 kb on - strand at 209.832 kb on - strand at 209.856 kb on + strand at 209.856 kb on + strand at 209.864 kb on - strand at 209.930 kb on - strand, within ldcC at 209.944 kb on - strand, within ldcC at 209.960 kb on + strand, within ldcC at 209.960 kb on + strand, within ldcC at 209.961 kb on - strand, within ldcC at 209.994 kb on + strand, within ldcC at 209.997 kb on - strand, within ldcC at 210.032 kb on + strand, within ldcC at 210.098 kb on - strand, within ldcC at 210.202 kb on + strand, within ldcC at 210.251 kb on + strand, within ldcC at 210.375 kb on + strand, within ldcC at 210.375 kb on + strand, within ldcC at 210.398 kb on - strand, within ldcC at 210.425 kb on - strand, within ldcC at 210.440 kb on + strand, within ldcC at 210.458 kb on - strand, within ldcC at 210.549 kb on + strand, within ldcC at 210.557 kb on - strand, within ldcC at 210.612 kb on + strand, within ldcC at 210.612 kb on + strand, within ldcC at 210.614 kb on + strand, within ldcC at 210.619 kb on - strand, within ldcC at 210.620 kb on - strand, within ldcC at 210.626 kb on - strand, within ldcC at 210.708 kb on - strand, within ldcC at 210.718 kb on - strand, within ldcC at 210.801 kb on - strand, within ldcC at 210.837 kb on - strand, within ldcC at 210.860 kb on - strand, within ldcC at 210.860 kb on - strand, within ldcC at 210.861 kb on + strand, within ldcC at 210.863 kb on - strand, within ldcC at 210.866 kb on + strand, within ldcC at 210.874 kb on - strand, within ldcC at 210.887 kb on + strand, within ldcC at 210.887 kb on + strand, within ldcC at 210.958 kb on + strand, within ldcC at 211.000 kb on - strand, within ldcC at 211.190 kb on + strand, within ldcC at 211.246 kb on + strand, within ldcC at 211.246 kb on + strand, within ldcC at 211.267 kb on + strand, within ldcC at 211.402 kb on - strand, within ldcC at 211.445 kb on + strand, within ldcC at 211.494 kb on + strand, within ldcC at 211.611 kb on - strand at 211.618 kb on + strand at 211.767 kb on + strand at 211.775 kb on - strand at 211.860 kb on + strand at 211.953 kb on + strand, within yaeR at 212.075 kb on + strand, within yaeR at 212.100 kb on + strand, within yaeR at 212.129 kb on - strand, within yaeR at 212.165 kb on + strand, within yaeR at 212.193 kb on + strand, within yaeR at 212.193 kb on + strand, within yaeR at 212.229 kb on - strand at 212.252 kb on + strand at 212.252 kb on + strand at 212.291 kb on + strand at 212.291 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB + carbenicillin regrowth after 48hr remove 209,655 - +0.2 209,826 - +0.1 209,832 - +0.6 209,856 + +0.9 209,856 + +0.6 209,864 - +0.1 209,930 - ldcC b0186 0.12 +0.4 209,944 - ldcC b0186 0.12 +0.0 209,960 + ldcC b0186 0.13 +0.1 209,960 + ldcC b0186 0.13 -0.3 209,961 - ldcC b0186 0.13 +0.1 209,994 + ldcC b0186 0.15 +0.3 209,997 - ldcC b0186 0.15 +0.4 210,032 + ldcC b0186 0.16 +0.7 210,098 - ldcC b0186 0.20 +0.3 210,202 + ldcC b0186 0.24 +0.4 210,251 + ldcC b0186 0.27 -0.1 210,375 + ldcC b0186 0.32 +0.4 210,375 + ldcC b0186 0.32 +0.2 210,398 - ldcC b0186 0.34 +0.1 210,425 - ldcC b0186 0.35 +0.2 210,440 + ldcC b0186 0.36 +0.2 210,458 - ldcC b0186 0.36 -0.0 210,549 + ldcC b0186 0.41 -0.0 210,557 - ldcC b0186 0.41 +0.4 210,612 + ldcC b0186 0.44 +0.3 210,612 + ldcC b0186 0.44 +0.6 210,614 + ldcC b0186 0.44 +0.7 210,619 - ldcC b0186 0.44 +0.2 210,620 - ldcC b0186 0.44 +0.1 210,626 - ldcC b0186 0.44 -0.1 210,708 - ldcC b0186 0.48 +0.2 210,718 - ldcC b0186 0.49 +0.3 210,801 - ldcC b0186 0.52 -0.0 210,837 - ldcC b0186 0.54 +0.1 210,860 - ldcC b0186 0.55 -0.1 210,860 - ldcC b0186 0.55 +0.6 210,861 + ldcC b0186 0.55 -0.4 210,863 - ldcC b0186 0.55 -0.4 210,866 + ldcC b0186 0.55 +0.6 210,874 - ldcC b0186 0.56 +0.3 210,887 + ldcC b0186 0.56 +0.2 210,887 + ldcC b0186 0.56 +0.1 210,958 + ldcC b0186 0.60 +0.3 211,000 - ldcC b0186 0.62 +0.2 211,190 + ldcC b0186 0.71 +0.3 211,246 + ldcC b0186 0.73 -0.1 211,246 + ldcC b0186 0.73 +0.5 211,267 + ldcC b0186 0.74 +0.1 211,402 - ldcC b0186 0.80 +0.6 211,445 + ldcC b0186 0.82 +0.6 211,494 + ldcC b0186 0.85 +0.1 211,611 - +0.3 211,618 + +0.9 211,767 + +0.1 211,775 - -0.0 211,860 + +0.1 211,953 + yaeR b0187 0.19 +0.3 212,075 + yaeR b0187 0.51 +0.6 212,100 + yaeR b0187 0.57 +0.2 212,129 - yaeR b0187 0.65 +0.0 212,165 + yaeR b0187 0.74 +0.2 212,193 + yaeR b0187 0.81 +0.7 212,193 + yaeR b0187 0.81 +0.3 212,229 - +0.4 212,252 + -0.8 212,252 + -1.0 212,291 + +0.6 212,291 + +0.3
Or see this region's nucleotide sequence