Strain Fitness in Escherichia coli BW25113 around b2022

Experiment: LB + ciprofloxacin Time9hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthisC and hisB overlap by 1 nucleotideshisB and hisH overlap by 1 nucleotideshisH and hisA overlap by 1 nucleotides b2021: hisC - histidinol-phosphate aminotransferase (NCBI), at 2,090,422 to 2,091,492 hisC b2022: hisB - imidazoleglycerolphosphate dehydratase and histidinol-phosphate phosphatase (VIMSS), at 2,091,492 to 2,092,559 hisB b2023: hisH - imidazole glycerol phosphate synthase subunit HisH (NCBI), at 2,092,559 to 2,093,149 hisH b2024: hisA - N-(5'-phospho-L-ribosyl-formimino)-5-amino-1-(5'- phosphoribosyl)-4-imidazolecarboxamide isomerase (VIMSS), at 2,093,149 to 2,093,886 hisA Position (kb) 2091 2092 2093Strain fitness (log2 ratio) -1 0 1at 2090.721 kb on - strand, within hisCat 2090.808 kb on - strand, within hisCat 2090.932 kb on + strand, within hisCat 2090.932 kb on + strand, within hisCat 2090.938 kb on + strand, within hisCat 2091.102 kb on + strand, within hisCat 2091.202 kb on - strand, within hisCat 2091.202 kb on - strand, within hisCat 2091.274 kb on + strand, within hisCat 2091.351 kb on + strand, within hisCat 2091.593 kb on - strandat 2091.593 kb on - strandat 2091.619 kb on - strand, within hisBat 2091.659 kb on + strand, within hisBat 2091.985 kb on - strand, within hisBat 2092.013 kb on - strand, within hisBat 2092.013 kb on - strand, within hisBat 2092.070 kb on + strand, within hisBat 2092.080 kb on + strand, within hisBat 2092.114 kb on - strand, within hisBat 2092.160 kb on + strand, within hisBat 2092.171 kb on - strand, within hisBat 2092.193 kb on - strand, within hisBat 2092.193 kb on - strand, within hisBat 2092.355 kb on + strand, within hisBat 2092.355 kb on + strand, within hisBat 2092.359 kb on - strand, within hisBat 2092.406 kb on + strand, within hisBat 2092.406 kb on + strand, within hisBat 2092.412 kb on + strand, within hisBat 2092.412 kb on + strand, within hisBat 2092.464 kb on + strandat 2092.533 kb on - strandat 2092.549 kb on - strandat 2092.752 kb on - strand, within hisHat 2092.772 kb on - strand, within hisHat 2092.907 kb on + strand, within hisHat 2093.026 kb on + strand, within hisHat 2093.026 kb on + strand, within hisHat 2093.071 kb on + strand, within hisHat 2093.071 kb on + strand, within hisHat 2093.079 kb on - strand, within hisHat 2093.079 kb on - strand, within hisHat 2093.079 kb on - strand, within hisHat 2093.079 kb on - strand, within hisHat 2093.094 kb on - strandat 2093.183 kb on + strandat 2093.183 kb on + strandat 2093.454 kb on - strand, within hisAat 2093.510 kb on - strand, within hisA

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time9hr
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2,090,721 - hisC b2021 0.28 +0.3
2,090,808 - hisC b2021 0.36 -0.0
2,090,932 + hisC b2021 0.48 +0.1
2,090,932 + hisC b2021 0.48 -0.1
2,090,938 + hisC b2021 0.48 +0.0
2,091,102 + hisC b2021 0.63 +0.3
2,091,202 - hisC b2021 0.73 +0.2
2,091,202 - hisC b2021 0.73 -0.8
2,091,274 + hisC b2021 0.80 -0.5
2,091,351 + hisC b2021 0.87 -0.3
2,091,593 - -0.1
2,091,593 - +0.3
2,091,619 - hisB b2022 0.12 -0.0
2,091,659 + hisB b2022 0.16 +0.3
2,091,985 - hisB b2022 0.46 +0.1
2,092,013 - hisB b2022 0.49 -0.2
2,092,013 - hisB b2022 0.49 +0.3
2,092,070 + hisB b2022 0.54 +0.4
2,092,080 + hisB b2022 0.55 -0.3
2,092,114 - hisB b2022 0.58 +0.5
2,092,160 + hisB b2022 0.63 -0.0
2,092,171 - hisB b2022 0.64 +1.6
2,092,193 - hisB b2022 0.66 +0.5
2,092,193 - hisB b2022 0.66 -0.3
2,092,355 + hisB b2022 0.81 +0.4
2,092,355 + hisB b2022 0.81 +0.4
2,092,359 - hisB b2022 0.81 +0.1
2,092,406 + hisB b2022 0.86 +1.1
2,092,406 + hisB b2022 0.86 +0.2
2,092,412 + hisB b2022 0.86 +1.0
2,092,412 + hisB b2022 0.86 -0.5
2,092,464 + -0.1
2,092,533 - +0.0
2,092,549 - +0.6
2,092,752 - hisH b2023 0.33 +0.3
2,092,772 - hisH b2023 0.36 +0.6
2,092,907 + hisH b2023 0.59 +0.1
2,093,026 + hisH b2023 0.79 +0.0
2,093,026 + hisH b2023 0.79 +0.1
2,093,071 + hisH b2023 0.87 -0.1
2,093,071 + hisH b2023 0.87 +0.5
2,093,079 - hisH b2023 0.88 -0.0
2,093,079 - hisH b2023 0.88 +0.7
2,093,079 - hisH b2023 0.88 +0.8
2,093,079 - hisH b2023 0.88 +0.7
2,093,094 - +0.2
2,093,183 + +0.0
2,093,183 + +1.5
2,093,454 - hisA b2024 0.41 -0.3
2,093,510 - hisA b2024 0.49 -0.0

Or see this region's nucleotide sequence