Experiment: LB + ciprofloxacin Time9hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ppiD and ybaV are separated by 150 nucleotides ybaV and ybaW are separated by 93 nucleotides ybaW and ybaX are separated by 51 nucleotides ybaX and ybaE are separated by 64 nucleotides
b0441: ppiD - peptidyl-prolyl cis-trans isomerase (rotamase D) (NCBI), at 461,139 to 463,010
ppiD
b0442: ybaV - hypothetical protein (NCBI), at 463,161 to 463,532
ybaV
b0443: ybaW - hypothetical protein (NCBI), at 463,626 to 464,024
ybaW
b0444: ybaX - predicted aluminum resistance protein (NCBI), at 464,076 to 464,771
ybaX
b0445: ybaE - predicted transporter subunit: periplasmic-binding component of ABC superfamily (NCBI), at 464,836 to 466,536
ybaE
Position (kb) 463
464
465 Strain fitness (log2 ratio) -1
0
1 at 462.682 kb on - strand, within ppiD at 462.696 kb on - strand, within ppiD at 462.696 kb on - strand, within ppiD at 462.720 kb on + strand, within ppiD at 462.816 kb on + strand, within ppiD at 462.816 kb on + strand, within ppiD at 462.833 kb on - strand at 462.833 kb on - strand at 462.903 kb on + strand at 462.903 kb on + strand at 462.918 kb on - strand at 462.923 kb on - strand at 463.049 kb on + strand at 463.049 kb on + strand at 463.173 kb on + strand at 463.173 kb on + strand at 463.182 kb on - strand at 463.184 kb on + strand at 463.184 kb on + strand at 463.184 kb on + strand at 463.184 kb on + strand at 463.186 kb on + strand at 463.192 kb on + strand at 463.192 kb on - strand at 463.192 kb on - strand at 463.193 kb on - strand at 463.204 kb on - strand, within ybaV at 463.205 kb on - strand, within ybaV at 463.205 kb on - strand, within ybaV at 463.288 kb on - strand, within ybaV at 463.303 kb on + strand, within ybaV at 463.438 kb on + strand, within ybaV at 463.465 kb on + strand, within ybaV at 463.473 kb on - strand, within ybaV at 463.483 kb on - strand, within ybaV at 463.518 kb on + strand at 463.590 kb on + strand at 463.608 kb on + strand at 463.655 kb on + strand at 463.655 kb on + strand at 463.761 kb on + strand, within ybaW at 463.761 kb on + strand, within ybaW at 463.775 kb on - strand, within ybaW at 463.775 kb on - strand, within ybaW at 463.826 kb on - strand, within ybaW at 463.879 kb on + strand, within ybaW at 463.879 kb on + strand, within ybaW at 463.897 kb on - strand, within ybaW at 463.911 kb on + strand, within ybaW at 463.920 kb on - strand, within ybaW at 463.920 kb on - strand, within ybaW at 463.977 kb on + strand, within ybaW at 463.977 kb on + strand, within ybaW at 463.977 kb on + strand, within ybaW at 463.977 kb on + strand, within ybaW at 463.979 kb on - strand, within ybaW at 463.986 kb on + strand at 463.986 kb on + strand at 464.035 kb on + strand at 464.035 kb on + strand at 464.209 kb on - strand, within ybaX at 464.209 kb on - strand, within ybaX at 464.226 kb on - strand, within ybaX at 464.230 kb on + strand, within ybaX at 464.287 kb on - strand, within ybaX at 464.332 kb on - strand, within ybaX at 464.332 kb on - strand, within ybaX at 464.344 kb on - strand, within ybaX at 464.346 kb on - strand, within ybaX at 464.346 kb on - strand, within ybaX at 464.433 kb on - strand, within ybaX at 464.433 kb on - strand, within ybaX at 464.511 kb on - strand, within ybaX at 464.511 kb on - strand, within ybaX at 464.523 kb on + strand, within ybaX at 464.523 kb on + strand, within ybaX at 464.551 kb on + strand, within ybaX at 464.553 kb on - strand, within ybaX at 464.553 kb on - strand, within ybaX at 464.557 kb on - strand, within ybaX at 464.557 kb on - strand, within ybaX at 464.559 kb on - strand, within ybaX at 464.569 kb on + strand, within ybaX at 464.579 kb on + strand, within ybaX at 464.582 kb on - strand, within ybaX at 464.589 kb on - strand, within ybaX at 464.594 kb on - strand, within ybaX at 464.675 kb on - strand, within ybaX at 464.708 kb on - strand at 464.772 kb on + strand at 464.772 kb on + strand at 464.803 kb on + strand at 464.926 kb on + strand at 464.969 kb on + strand at 464.969 kb on + strand at 464.977 kb on - strand at 464.977 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time9hr remove 462,682 - ppiD b0441 0.82 +0.2 462,696 - ppiD b0441 0.83 +0.4 462,696 - ppiD b0441 0.83 +0.5 462,720 + ppiD b0441 0.84 +0.7 462,816 + ppiD b0441 0.90 +0.2 462,816 + ppiD b0441 0.90 -0.3 462,833 - +0.4 462,833 - +0.5 462,903 + +0.0 462,903 + +0.0 462,918 - -0.2 462,923 - -0.5 463,049 + +0.1 463,049 + +0.5 463,173 + +0.2 463,173 + +0.7 463,182 - +0.5 463,184 + +0.1 463,184 + -0.4 463,184 + +0.1 463,184 + -0.0 463,186 + +0.0 463,192 + +0.0 463,192 - +0.8 463,192 - +0.0 463,193 - -0.2 463,204 - ybaV b0442 0.12 -0.5 463,205 - ybaV b0442 0.12 +0.4 463,205 - ybaV b0442 0.12 +0.5 463,288 - ybaV b0442 0.34 +0.4 463,303 + ybaV b0442 0.38 +0.4 463,438 + ybaV b0442 0.74 +0.2 463,465 + ybaV b0442 0.82 +0.1 463,473 - ybaV b0442 0.84 +0.1 463,483 - ybaV b0442 0.87 +0.5 463,518 + +0.7 463,590 + +0.3 463,608 + +0.0 463,655 + -0.3 463,655 + +0.5 463,761 + ybaW b0443 0.34 +0.6 463,761 + ybaW b0443 0.34 +1.0 463,775 - ybaW b0443 0.37 +0.4 463,775 - ybaW b0443 0.37 +1.4 463,826 - ybaW b0443 0.50 +0.1 463,879 + ybaW b0443 0.63 +0.5 463,879 + ybaW b0443 0.63 +0.6 463,897 - ybaW b0443 0.68 -0.2 463,911 + ybaW b0443 0.71 +0.4 463,920 - ybaW b0443 0.74 +0.5 463,920 - ybaW b0443 0.74 -0.3 463,977 + ybaW b0443 0.88 +0.2 463,977 + ybaW b0443 0.88 -0.1 463,977 + ybaW b0443 0.88 +0.3 463,977 + ybaW b0443 0.88 +0.2 463,979 - ybaW b0443 0.88 +0.7 463,986 + +0.5 463,986 + -0.1 464,035 + +0.2 464,035 + +0.6 464,209 - ybaX b0444 0.19 +0.6 464,209 - ybaX b0444 0.19 -0.5 464,226 - ybaX b0444 0.22 +0.4 464,230 + ybaX b0444 0.22 -0.3 464,287 - ybaX b0444 0.30 -0.0 464,332 - ybaX b0444 0.37 -0.1 464,332 - ybaX b0444 0.37 +0.1 464,344 - ybaX b0444 0.39 +0.4 464,346 - ybaX b0444 0.39 +0.2 464,346 - ybaX b0444 0.39 -0.1 464,433 - ybaX b0444 0.51 -0.4 464,433 - ybaX b0444 0.51 +0.4 464,511 - ybaX b0444 0.62 +0.1 464,511 - ybaX b0444 0.62 -0.0 464,523 + ybaX b0444 0.64 -0.1 464,523 + ybaX b0444 0.64 +0.1 464,551 + ybaX b0444 0.68 +0.5 464,553 - ybaX b0444 0.69 +0.5 464,553 - ybaX b0444 0.69 +1.2 464,557 - ybaX b0444 0.69 +0.3 464,557 - ybaX b0444 0.69 +0.3 464,559 - ybaX b0444 0.69 +0.3 464,569 + ybaX b0444 0.71 +0.2 464,579 + ybaX b0444 0.72 -0.2 464,582 - ybaX b0444 0.73 -1.5 464,589 - ybaX b0444 0.74 +0.1 464,594 - ybaX b0444 0.74 +0.3 464,675 - ybaX b0444 0.86 +0.1 464,708 - +0.2 464,772 + -0.0 464,772 + -0.1 464,803 + +0.2 464,926 + +0.2 464,969 + -0.1 464,969 + -0.5 464,977 - +0.1 464,977 - +0.4
Or see this region's nucleotide sequence