Strain Fitness in Escherichia coli BW25113 around b4492

Experiment: LB + carbenicillin Time8hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpaaX and paaY overlap by 19 nucleotidespaaY and ynbG are separated by 103 nucleotidesynbG and ydbA are separated by 161 nucleotidesydbA and insD-2 are separated by 16 nucleotidesinsD-2 and insC overlap by 43 nucleotides b1399: paaX - DNA-binding transcriptional repressor of phenylacetic acid degradation, aryl-CoA responsive (NCBI), at 1,461,563 to 1,462,513 paaX b1400: paaY - predicted hexapeptide repeat acetyltransferase (NCBI), at 1,462,495 to 1,463,085 paaY b4674: ynbG - expressed protein (RefSeq), at 1,463,189 to 1,463,254 ynbG b4492: ydbA - no description, at 1,463,416 to 1,465,928 ydbA b1402: insD-2 - IS2 insertion element transposase InsAB' (NCBI), at 1,465,945 to 1,466,850 insD-2 b1403: insC - KpLE2 phage-like element; IS2 insertion element repressor InsA (RefSeq), at 1,466,808 to 1,467,173 insC Position (kb) 1463 1464 1465 1466Strain fitness (log2 ratio) -1 0 1 2at 1462.521 kb on + strandat 1462.521 kb on + strandat 1462.975 kb on + strand, within paaYat 1462.975 kb on + strand, within paaYat 1462.988 kb on + strand, within paaYat 1463.000 kb on + strand, within paaYat 1463.053 kb on + strandat 1463.053 kb on + strandat 1463.080 kb on + strandat 1463.082 kb on + strandat 1463.499 kb on - strandat 1463.499 kb on - strandat 1463.754 kb on - strand, within ydbAat 1463.761 kb on - strand, within ydbAat 1463.762 kb on - strand, within ydbAat 1463.764 kb on - strand, within ydbAat 1463.764 kb on - strand, within ydbAat 1463.791 kb on + strand, within ydbAat 1463.801 kb on + strand, within ydbAat 1463.809 kb on - strand, within ydbAat 1463.813 kb on + strand, within ydbAat 1463.813 kb on + strand, within ydbAat 1463.813 kb on + strand, within ydbAat 1463.813 kb on + strand, within ydbAat 1463.821 kb on - strand, within ydbAat 1463.822 kb on - strand, within ydbAat 1463.874 kb on + strand, within ydbAat 1463.874 kb on + strand, within ydbAat 1463.924 kb on - strand, within ydbAat 1463.928 kb on - strand, within ydbAat 1463.932 kb on - strand, within ydbAat 1463.933 kb on - strand, within ydbAat 1463.971 kb on - strand, within ydbAat 1463.971 kb on - strand, within ydbAat 1463.989 kb on - strand, within ydbAat 1464.012 kb on - strand, within ydbAat 1464.023 kb on - strand, within ydbAat 1464.023 kb on - strand, within ydbAat 1464.026 kb on - strand, within ydbAat 1464.026 kb on - strand, within ydbAat 1464.091 kb on + strand, within ydbAat 1464.194 kb on - strand, within ydbAat 1464.194 kb on - strand, within ydbAat 1464.246 kb on + strandat 1464.254 kb on + strand, within ydbAat 1464.254 kb on + strand, within ydbAat 1464.283 kb on + strand, within ydbAat 1464.371 kb on - strand, within ydbAat 1464.385 kb on + strand, within ydbAat 1464.612 kb on - strand, within ydbAat 1465.027 kb on + strand, within ydbAat 1465.080 kb on - strand, within ydbAat 1465.080 kb on - strand, within ydbAat 1465.164 kb on - strand, within ydbAat 1465.179 kb on - strand, within ydbAat 1465.179 kb on - strand, within ydbAat 1465.239 kb on + strand, within ydbAat 1465.246 kb on + strand, within ydbAat 1465.307 kb on + strand, within ydbAat 1465.307 kb on + strand, within ydbAat 1465.318 kb on - strand, within ydbAat 1465.318 kb on - strand, within ydbAat 1465.415 kb on - strand, within ydbAat 1465.563 kb on + strand, within ydbAat 1465.638 kb on - strand, within ydbAat 1465.641 kb on + strand, within ydbAat 1465.669 kb on - strand, within ydbAat 1465.732 kb on + strandat 1465.732 kb on + strandat 1465.732 kb on + strandat 1465.925 kb on - strandat 1465.939 kb on - strandat 1465.939 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin Time8hr
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1,462,521 + +1.0
1,462,521 + +0.6
1,462,975 + paaY b1400 0.81 +0.4
1,462,975 + paaY b1400 0.81 -0.0
1,462,988 + paaY b1400 0.83 -0.0
1,463,000 + paaY b1400 0.85 +0.7
1,463,053 + +0.1
1,463,053 + -0.1
1,463,080 + -0.6
1,463,082 + +0.3
1,463,499 - +0.9
1,463,499 - -0.7
1,463,754 - ydbA b4492 0.13 +2.2
1,463,761 - ydbA b4492 0.14 +1.2
1,463,762 - ydbA b4492 0.14 +0.2
1,463,764 - ydbA b4492 0.14 -1.4
1,463,764 - ydbA b4492 0.14 +0.9
1,463,791 + ydbA b4492 0.15 +1.5
1,463,801 + ydbA b4492 0.15 +1.3
1,463,809 - ydbA b4492 0.16 +0.1
1,463,813 + ydbA b4492 0.16 -0.4
1,463,813 + ydbA b4492 0.16 +0.9
1,463,813 + ydbA b4492 0.16 +0.1
1,463,813 + ydbA b4492 0.16 -1.6
1,463,821 - ydbA b4492 0.16 +0.9
1,463,822 - ydbA b4492 0.16 +0.9
1,463,874 + ydbA b4492 0.18 +0.8
1,463,874 + ydbA b4492 0.18 +1.1
1,463,924 - ydbA b4492 0.20 +0.6
1,463,928 - ydbA b4492 0.20 +0.3
1,463,932 - ydbA b4492 0.21 -0.1
1,463,933 - ydbA b4492 0.21 +1.5
1,463,971 - ydbA b4492 0.22 +0.7
1,463,971 - ydbA b4492 0.22 +0.2
1,463,989 - ydbA b4492 0.23 +0.1
1,464,012 - ydbA b4492 0.24 +0.2
1,464,023 - ydbA b4492 0.24 +0.7
1,464,023 - ydbA b4492 0.24 -0.1
1,464,026 - ydbA b4492 0.24 +1.1
1,464,026 - ydbA b4492 0.24 -0.4
1,464,091 + ydbA b4492 0.27 +0.9
1,464,194 - ydbA b4492 0.31 +1.3
1,464,194 - ydbA b4492 0.31 +0.3
1,464,246 + -0.1
1,464,254 + ydbA b4492 0.33 -0.3
1,464,254 + ydbA b4492 0.33 +0.1
1,464,283 + ydbA b4492 0.35 +0.3
1,464,371 - ydbA b4492 0.38 +0.0
1,464,385 + ydbA b4492 0.39 +0.1
1,464,612 - ydbA b4492 0.48 -0.1
1,465,027 + ydbA b4492 0.64 -0.1
1,465,080 - ydbA b4492 0.66 -0.0
1,465,080 - ydbA b4492 0.66 -0.0
1,465,164 - ydbA b4492 0.70 +0.3
1,465,179 - ydbA b4492 0.70 +0.7
1,465,179 - ydbA b4492 0.70 +0.5
1,465,239 + ydbA b4492 0.73 +0.5
1,465,246 + ydbA b4492 0.73 +0.5
1,465,307 + ydbA b4492 0.75 +0.7
1,465,307 + ydbA b4492 0.75 +0.4
1,465,318 - ydbA b4492 0.76 +1.4
1,465,318 - ydbA b4492 0.76 -0.4
1,465,415 - ydbA b4492 0.80 +2.0
1,465,563 + ydbA b4492 0.85 -0.2
1,465,638 - ydbA b4492 0.88 +0.9
1,465,641 + ydbA b4492 0.89 +0.7
1,465,669 - ydbA b4492 0.90 -0.9
1,465,732 + +0.5
1,465,732 + +1.2
1,465,732 + +0.4
1,465,925 - +1.9
1,465,939 - -1.6
1,465,939 - +0.6

Or see this region's nucleotide sequence