Strain Fitness in Escherichia coli BW25113 around b2202

Experiment: LB + carbenicillin Time8hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntccmB and ccmA overlap by 4 nucleotidesccmA and napC are separated by 12 nucleotidesnapC and napB are separated by 9 nucleotidesnapB and napH overlap by 4 nucleotides b2200: ccmB - heme exporter subunit (NCBI), at 2,294,384 to 2,295,046 ccmB b2201: ccmA - ATP binding protein of heme exporter A (VIMSS), at 2,295,043 to 2,295,666 ccmA b2202: napC - nitrate reductase, cytochrome c-type, periplasmic (NCBI), at 2,295,679 to 2,296,281 napC b2203: napB - cytochrome c-type protein (VIMSS), at 2,296,291 to 2,296,740 napB b2204: napH - quinol dehydrogenase membrane component (NCBI), at 2,296,737 to 2,297,600 napH Position (kb) 2295 2296 2297Strain fitness (log2 ratio) -1 0 1at 2294.744 kb on - strand, within ccmBat 2294.744 kb on - strand, within ccmBat 2294.904 kb on - strand, within ccmBat 2294.928 kb on - strand, within ccmBat 2294.999 kb on + strandat 2295.133 kb on + strand, within ccmAat 2295.133 kb on + strand, within ccmAat 2295.152 kb on + strand, within ccmAat 2295.207 kb on + strand, within ccmAat 2295.207 kb on + strand, within ccmAat 2295.285 kb on + strand, within ccmAat 2295.401 kb on + strand, within ccmAat 2295.433 kb on - strand, within ccmAat 2295.476 kb on + strand, within ccmAat 2295.476 kb on + strand, within ccmAat 2295.516 kb on - strand, within ccmAat 2295.575 kb on - strand, within ccmAat 2295.650 kb on + strandat 2295.690 kb on + strandat 2295.722 kb on - strandat 2295.760 kb on + strand, within napCat 2295.760 kb on + strand, within napCat 2295.769 kb on + strand, within napCat 2295.806 kb on + strand, within napCat 2295.880 kb on + strand, within napCat 2295.921 kb on - strand, within napCat 2296.069 kb on - strand, within napCat 2296.154 kb on - strand, within napCat 2296.254 kb on + strandat 2296.254 kb on + strandat 2296.348 kb on + strand, within napBat 2296.348 kb on + strand, within napBat 2296.356 kb on + strand, within napBat 2296.356 kb on + strand, within napBat 2296.368 kb on + strand, within napBat 2296.431 kb on + strand, within napBat 2296.491 kb on + strand, within napBat 2296.491 kb on + strand, within napBat 2296.491 kb on - strand, within napBat 2296.545 kb on + strand, within napBat 2296.578 kb on + strand, within napBat 2296.578 kb on + strand, within napBat 2296.675 kb on + strand, within napBat 2296.695 kb on - strand, within napBat 2296.773 kb on + strandat 2296.773 kb on + strandat 2296.779 kb on - strandat 2296.880 kb on - strand, within napHat 2296.953 kb on + strand, within napHat 2297.095 kb on - strand, within napH

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin Time8hr
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2,294,744 - ccmB b2200 0.54 -0.5
2,294,744 - ccmB b2200 0.54 +0.1
2,294,904 - ccmB b2200 0.78 +0.3
2,294,928 - ccmB b2200 0.82 -0.2
2,294,999 + +1.0
2,295,133 + ccmA b2201 0.14 -0.3
2,295,133 + ccmA b2201 0.14 +0.8
2,295,152 + ccmA b2201 0.17 +0.1
2,295,207 + ccmA b2201 0.26 +0.6
2,295,207 + ccmA b2201 0.26 -0.3
2,295,285 + ccmA b2201 0.39 +0.0
2,295,401 + ccmA b2201 0.57 -0.1
2,295,433 - ccmA b2201 0.62 +0.3
2,295,476 + ccmA b2201 0.69 -0.1
2,295,476 + ccmA b2201 0.69 +0.5
2,295,516 - ccmA b2201 0.76 -0.1
2,295,575 - ccmA b2201 0.85 -0.1
2,295,650 + +0.1
2,295,690 + -0.3
2,295,722 - -0.1
2,295,760 + napC b2202 0.13 +0.3
2,295,760 + napC b2202 0.13 +1.0
2,295,769 + napC b2202 0.15 +0.6
2,295,806 + napC b2202 0.21 -0.2
2,295,880 + napC b2202 0.33 +0.4
2,295,921 - napC b2202 0.40 +0.1
2,296,069 - napC b2202 0.65 +0.5
2,296,154 - napC b2202 0.79 -0.1
2,296,254 + +0.0
2,296,254 + -0.1
2,296,348 + napB b2203 0.13 +0.2
2,296,348 + napB b2203 0.13 +0.1
2,296,356 + napB b2203 0.14 +0.3
2,296,356 + napB b2203 0.14 +0.0
2,296,368 + napB b2203 0.17 +0.6
2,296,431 + napB b2203 0.31 +0.0
2,296,491 + napB b2203 0.44 +0.5
2,296,491 + napB b2203 0.44 +0.1
2,296,491 - napB b2203 0.44 -0.0
2,296,545 + napB b2203 0.56 +0.2
2,296,578 + napB b2203 0.64 +0.6
2,296,578 + napB b2203 0.64 +0.6
2,296,675 + napB b2203 0.85 -0.1
2,296,695 - napB b2203 0.90 -0.1
2,296,773 + +0.3
2,296,773 + +0.5
2,296,779 - +0.2
2,296,880 - napH b2204 0.17 +0.3
2,296,953 + napH b2204 0.25 +1.0
2,297,095 - napH b2204 0.41 -0.1

Or see this region's nucleotide sequence