Experiment: LB + carbenicillin Time8hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yeaJ and yeaK are separated by 42 nucleotides yeaK and b1788 are separated by 0 nucleotides b1788 and yeaL are separated by 169 nucleotides yeaL and yeaM overlap by 44 nucleotides yeaM and yeaN are separated by 96 nucleotides
b1786: yeaJ - orf, hypothetical protein (VIMSS), at 1,870,065 to 1,871,555
yeaJ
b1787: yeaK - hypothetical protein (NCBI), at 1,871,598 to 1,872,101
yeaK
b1788: b1788 - orf, hypothetical protein (VIMSS), at 1,872,102 to 1,872,206
b1788
b1789: yeaL - conserved inner membrane protein (NCBI), at 1,872,376 to 1,872,822
yeaL
b1790: yeaM - predicted DNA-binding transcriptional regulator (NCBI), at 1,872,779 to 1,873,600
yeaM
b1791: yeaN - predicted transporter (NCBI), at 1,873,697 to 1,874,878
yeaN
Position (kb)
1872
1873 Strain fitness (log2 ratio)
-1
0
1 at 1871.390 kb on + strand, within yeaJ at 1871.396 kb on - strand, within yeaJ at 1871.414 kb on + strand at 1871.414 kb on + strand at 1871.436 kb on + strand at 1871.483 kb on + strand at 1871.495 kb on + strand at 1871.495 kb on + strand at 1871.502 kb on - strand at 1871.534 kb on + strand at 1871.534 kb on + strand at 1871.534 kb on + strand at 1871.534 kb on + strand at 1871.551 kb on + strand at 1871.551 kb on + strand at 1871.581 kb on + strand at 1871.581 kb on + strand at 1871.734 kb on + strand, within yeaK at 1871.741 kb on - strand, within yeaK at 1871.746 kb on - strand, within yeaK at 1871.748 kb on + strand, within yeaK at 1871.748 kb on + strand, within yeaK at 1871.754 kb on - strand, within yeaK at 1871.754 kb on - strand, within yeaK at 1871.759 kb on + strand, within yeaK at 1871.790 kb on - strand, within yeaK at 1871.804 kb on - strand, within yeaK at 1871.823 kb on - strand, within yeaK at 1871.909 kb on - strand, within yeaK at 1871.909 kb on - strand, within yeaK at 1871.978 kb on - strand, within yeaK at 1872.285 kb on - strand at 1872.399 kb on - strand at 1872.443 kb on - strand, within yeaL at 1872.584 kb on + strand, within yeaL at 1872.606 kb on - strand, within yeaL at 1872.672 kb on - strand, within yeaL at 1872.672 kb on - strand, within yeaL at 1872.680 kb on + strand, within yeaL at 1872.680 kb on + strand, within yeaL at 1873.148 kb on + strand, within yeaM at 1873.370 kb on + strand, within yeaM at 1873.391 kb on - strand, within yeaM at 1873.472 kb on + strand, within yeaM at 1873.472 kb on + strand, within yeaM at 1873.510 kb on + strand, within yeaM at 1873.559 kb on - strand at 1873.744 kb on - strand at 1873.791 kb on + strand at 1873.799 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB + carbenicillin Time8hr remove 1,871,390 + yeaJ b1786 0.89 +0.3 1,871,396 - yeaJ b1786 0.89 +0.2 1,871,414 + -0.2 1,871,414 + +0.7 1,871,436 + -0.2 1,871,483 + -0.5 1,871,495 + -0.7 1,871,495 + -0.7 1,871,502 - -0.3 1,871,534 + -0.1 1,871,534 + +0.9 1,871,534 + +0.9 1,871,534 + -0.7 1,871,551 + +0.2 1,871,551 + +0.2 1,871,581 + -0.7 1,871,581 + +1.2 1,871,734 + yeaK b1787 0.27 -0.3 1,871,741 - yeaK b1787 0.28 +0.4 1,871,746 - yeaK b1787 0.29 +0.1 1,871,748 + yeaK b1787 0.30 +0.5 1,871,748 + yeaK b1787 0.30 -0.3 1,871,754 - yeaK b1787 0.31 -0.2 1,871,754 - yeaK b1787 0.31 -1.5 1,871,759 + yeaK b1787 0.32 +0.1 1,871,790 - yeaK b1787 0.38 +0.8 1,871,804 - yeaK b1787 0.41 -0.2 1,871,823 - yeaK b1787 0.45 +0.1 1,871,909 - yeaK b1787 0.62 -0.2 1,871,909 - yeaK b1787 0.62 +1.4 1,871,978 - yeaK b1787 0.75 +0.3 1,872,285 - -0.0 1,872,399 - +0.2 1,872,443 - yeaL b1789 0.15 +1.0 1,872,584 + yeaL b1789 0.47 +0.5 1,872,606 - yeaL b1789 0.51 -0.5 1,872,672 - yeaL b1789 0.66 +0.2 1,872,672 - yeaL b1789 0.66 -0.1 1,872,680 + yeaL b1789 0.68 +0.8 1,872,680 + yeaL b1789 0.68 +0.2 1,873,148 + yeaM b1790 0.45 +0.4 1,873,370 + yeaM b1790 0.72 -0.1 1,873,391 - yeaM b1790 0.74 -0.3 1,873,472 + yeaM b1790 0.84 +0.2 1,873,472 + yeaM b1790 0.84 +0.8 1,873,510 + yeaM b1790 0.89 +1.5 1,873,559 - +0.4 1,873,744 - -0.1 1,873,791 + +1.0 1,873,799 - +1.2
Or see this region's nucleotide sequence