Strain Fitness in Escherichia coli BW25113 around b1453

Experiment: LB + carbenicillin Time8hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyncE and ansP are separated by 112 nucleotidesansP and yncG are separated by 266 nucleotidesyncG and yncH are separated by 75 nucleotides b1452: yncE - hypothetical protein (NCBI), at 1,521,331 to 1,522,392 yncE b1453: ansP - L-asparagine permease (VIMSS), at 1,522,505 to 1,524,004 ansP b1454: yncG - predicted enzyme (NCBI), at 1,524,271 to 1,524,888 yncG b1455: yncH - hypothetical protein (NCBI), at 1,524,964 to 1,525,176 yncH Position (kb) 1522 1523 1524 1525Strain fitness (log2 ratio) -1 0 1at 1521.525 kb on + strand, within yncEat 1521.525 kb on + strand, within yncEat 1521.556 kb on - strand, within yncEat 1521.593 kb on + strand, within yncEat 1521.593 kb on + strand, within yncEat 1521.642 kb on + strand, within yncEat 1521.660 kb on - strand, within yncEat 1521.660 kb on - strand, within yncEat 1521.932 kb on - strand, within yncEat 1522.035 kb on + strand, within yncEat 1522.067 kb on - strand, within yncEat 1522.186 kb on + strand, within yncEat 1522.403 kb on - strandat 1522.414 kb on - strandat 1522.664 kb on - strand, within ansPat 1522.740 kb on + strand, within ansPat 1522.856 kb on + strand, within ansPat 1523.330 kb on - strand, within ansPat 1523.402 kb on - strand, within ansPat 1523.402 kb on - strand, within ansPat 1523.604 kb on + strand, within ansPat 1523.604 kb on + strand, within ansPat 1523.629 kb on + strand, within ansPat 1523.902 kb on - strandat 1524.042 kb on - strandat 1524.042 kb on - strandat 1524.133 kb on - strandat 1524.133 kb on - strandat 1524.232 kb on - strandat 1524.235 kb on - strandat 1524.242 kb on - strandat 1524.288 kb on - strandat 1524.290 kb on - strandat 1524.432 kb on + strand, within yncGat 1524.472 kb on + strand, within yncGat 1524.574 kb on + strand, within yncGat 1524.609 kb on + strand, within yncGat 1524.632 kb on + strand, within yncGat 1524.704 kb on + strand, within yncGat 1524.753 kb on + strand, within yncGat 1524.753 kb on + strand, within yncGat 1524.763 kb on + strand, within yncGat 1524.763 kb on + strand, within yncGat 1524.768 kb on + strand, within yncG

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin Time8hr
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1,521,525 + yncE b1452 0.18 +0.4
1,521,525 + yncE b1452 0.18 +0.3
1,521,556 - yncE b1452 0.21 +0.3
1,521,593 + yncE b1452 0.25 -0.4
1,521,593 + yncE b1452 0.25 +0.3
1,521,642 + yncE b1452 0.29 +0.2
1,521,660 - yncE b1452 0.31 -0.5
1,521,660 - yncE b1452 0.31 +0.9
1,521,932 - yncE b1452 0.57 +0.5
1,522,035 + yncE b1452 0.66 -0.2
1,522,067 - yncE b1452 0.69 +0.6
1,522,186 + yncE b1452 0.81 +0.5
1,522,403 - -1.1
1,522,414 - +0.5
1,522,664 - ansP b1453 0.11 +0.3
1,522,740 + ansP b1453 0.16 +0.4
1,522,856 + ansP b1453 0.23 +0.6
1,523,330 - ansP b1453 0.55 +0.1
1,523,402 - ansP b1453 0.60 +0.2
1,523,402 - ansP b1453 0.60 +0.1
1,523,604 + ansP b1453 0.73 +1.0
1,523,604 + ansP b1453 0.73 -0.2
1,523,629 + ansP b1453 0.75 +0.7
1,523,902 - +0.7
1,524,042 - +0.1
1,524,042 - +0.1
1,524,133 - -0.4
1,524,133 - +0.3
1,524,232 - +0.3
1,524,235 - -0.0
1,524,242 - +1.2
1,524,288 - +0.3
1,524,290 - +0.1
1,524,432 + yncG b1454 0.26 +0.8
1,524,472 + yncG b1454 0.33 -0.7
1,524,574 + yncG b1454 0.49 -0.5
1,524,609 + yncG b1454 0.55 -0.3
1,524,632 + yncG b1454 0.58 +0.9
1,524,704 + yncG b1454 0.70 -0.1
1,524,753 + yncG b1454 0.78 +0.9
1,524,753 + yncG b1454 0.78 -0.3
1,524,763 + yncG b1454 0.80 +0.4
1,524,763 + yncG b1454 0.80 +0.4
1,524,768 + yncG b1454 0.80 -0.1

Or see this region's nucleotide sequence