Strain Fitness in Escherichia coli BW25113 around b1414

Experiment: LB + carbenicillin Time8hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthrpA and ydcF are separated by 271 nucleotidesydcF and aldA are separated by 196 nucleotides b1413: hrpA - ATP-dependent helicase (RefSeq), at 1,481,085 to 1,484,987 hrpA b1414: ydcF - hypothetical protein (NCBI), at 1,485,259 to 1,486,059 ydcF b1415: aldA - Lactaldehyde / glycolaldehyde dehydrogenase aldA (EC 1.2.1.22; EC 1.2.1.21) (from data), at 1,486,256 to 1,487,695 aldA Position (kb) 1485 1486 1487Strain fitness (log2 ratio) -1 0 1at 1484.433 kb on + strand, within hrpAat 1484.566 kb on - strand, within hrpAat 1484.615 kb on - strandat 1484.883 kb on + strandat 1484.883 kb on + strandat 1485.446 kb on + strand, within ydcFat 1485.502 kb on + strand, within ydcFat 1485.553 kb on - strand, within ydcFat 1485.833 kb on + strand, within ydcFat 1485.869 kb on - strand, within ydcFat 1485.881 kb on + strand, within ydcFat 1485.881 kb on + strand, within ydcFat 1485.887 kb on - strand, within ydcFat 1485.899 kb on - strand, within ydcFat 1485.899 kb on - strand, within ydcFat 1486.182 kb on - strandat 1486.274 kb on - strandat 1486.276 kb on - strandat 1486.276 kb on - strandat 1486.392 kb on + strandat 1486.392 kb on + strandat 1486.401 kb on - strand, within aldAat 1486.401 kb on - strand, within aldAat 1486.721 kb on - strand, within aldAat 1486.932 kb on + strand, within aldAat 1486.932 kb on + strand, within aldA

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin Time8hr
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1,484,433 + hrpA b1413 0.86 +0.2
1,484,566 - hrpA b1413 0.89 -0.3
1,484,615 - -0.7
1,484,883 + -0.2
1,484,883 + +0.5
1,485,446 + ydcF b1414 0.23 +0.9
1,485,502 + ydcF b1414 0.30 +0.2
1,485,553 - ydcF b1414 0.37 -0.0
1,485,833 + ydcF b1414 0.72 +0.1
1,485,869 - ydcF b1414 0.76 +0.7
1,485,881 + ydcF b1414 0.78 +1.1
1,485,881 + ydcF b1414 0.78 +0.6
1,485,887 - ydcF b1414 0.78 +0.6
1,485,899 - ydcF b1414 0.80 +0.4
1,485,899 - ydcF b1414 0.80 -0.2
1,486,182 - +0.5
1,486,274 - -0.1
1,486,276 - +0.2
1,486,276 - -0.1
1,486,392 + +1.2
1,486,392 + +0.9
1,486,401 - aldA b1415 0.10 +0.3
1,486,401 - aldA b1415 0.10 +0.6
1,486,721 - aldA b1415 0.32 +0.6
1,486,932 + aldA b1415 0.47 +0.1
1,486,932 + aldA b1415 0.47 +0.2

Or see this region's nucleotide sequence