Experiment: LB + carbenicillin Time8hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sucD and mngR are separated by 103 nucleotides mngR and mngA are separated by 108 nucleotides mngA and mngB are separated by 17 nucleotides
b0729: sucD - succinyl-CoA synthetase subunit alpha (NCBI), at 763,403 to 764,272
sucD
b0730: mngR - DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI), at 764,376 to 765,098
mngR
b0731: mngA - fused 2-O-a-mannosyl-D-glycerate specific PTS enzymes: IIA component/IIB component/IIC component (NCBI), at 765,207 to 767,183
mngA
b0732: mngB - alpha-mannosidase (NCBI), at 767,201 to 769,834
mngB
Position (kb) 765
766
767
768 Strain fitness (log2 ratio) -1
0
1
2
3 at 764.252 kb on - strand at 764.279 kb on + strand at 764.302 kb on + strand at 764.302 kb on + strand at 764.311 kb on + strand at 764.319 kb on + strand at 764.349 kb on + strand at 764.480 kb on + strand, within mngR at 764.480 kb on + strand, within mngR at 764.515 kb on + strand, within mngR at 764.515 kb on + strand, within mngR at 764.515 kb on + strand, within mngR at 764.515 kb on + strand, within mngR at 764.645 kb on + strand, within mngR at 764.668 kb on + strand, within mngR at 764.811 kb on + strand, within mngR at 765.279 kb on + strand at 765.424 kb on + strand, within mngA at 765.590 kb on + strand, within mngA at 765.590 kb on + strand, within mngA at 765.674 kb on + strand, within mngA at 765.674 kb on - strand, within mngA at 765.675 kb on - strand, within mngA at 765.803 kb on - strand, within mngA at 765.803 kb on - strand, within mngA at 765.811 kb on - strand, within mngA at 765.811 kb on - strand, within mngA at 765.960 kb on - strand, within mngA at 766.059 kb on - strand, within mngA at 766.059 kb on - strand, within mngA at 766.066 kb on - strand, within mngA at 766.156 kb on - strand, within mngA at 766.156 kb on - strand, within mngA at 766.185 kb on + strand, within mngA at 766.263 kb on + strand, within mngA at 766.399 kb on - strand, within mngA at 766.767 kb on + strand, within mngA at 766.767 kb on + strand, within mngA at 766.848 kb on + strand, within mngA at 766.848 kb on + strand, within mngA at 767.053 kb on + strand at 767.053 kb on + strand at 767.053 kb on + strand at 767.158 kb on + strand at 767.158 kb on + strand at 767.226 kb on - strand at 767.257 kb on + strand at 767.309 kb on + strand at 767.329 kb on + strand at 767.329 kb on + strand at 767.473 kb on - strand, within mngB at 767.473 kb on - strand, within mngB at 767.473 kb on - strand, within mngB at 767.473 kb on - strand, within mngB at 767.535 kb on - strand, within mngB at 767.614 kb on - strand, within mngB at 767.614 kb on - strand, within mngB at 767.655 kb on + strand, within mngB at 767.717 kb on - strand, within mngB at 767.863 kb on + strand, within mngB at 767.917 kb on + strand, within mngB at 767.917 kb on + strand, within mngB at 767.917 kb on + strand, within mngB at 767.925 kb on - strand, within mngB at 767.937 kb on - strand, within mngB at 767.937 kb on - strand, within mngB at 768.026 kb on + strand, within mngB at 768.124 kb on + strand, within mngB at 768.131 kb on + strand, within mngB at 768.135 kb on - strand, within mngB at 768.135 kb on - strand, within mngB
Per-strain Table
Position Strand Gene LocusTag Fraction LB + carbenicillin Time8hr remove 764,252 - -1.5 764,279 + +0.6 764,302 + +0.0 764,302 + +0.2 764,311 + +0.3 764,319 + +0.3 764,349 + +0.1 764,480 + mngR b0730 0.14 +1.5 764,480 + mngR b0730 0.14 +0.8 764,515 + mngR b0730 0.19 -0.2 764,515 + mngR b0730 0.19 +1.1 764,515 + mngR b0730 0.19 +2.8 764,515 + mngR b0730 0.19 +0.8 764,645 + mngR b0730 0.37 +0.5 764,668 + mngR b0730 0.40 +1.8 764,811 + mngR b0730 0.60 +0.8 765,279 + +0.1 765,424 + mngA b0731 0.11 +0.2 765,590 + mngA b0731 0.19 +0.3 765,590 + mngA b0731 0.19 +0.0 765,674 + mngA b0731 0.24 -0.1 765,674 - mngA b0731 0.24 +0.2 765,675 - mngA b0731 0.24 +1.2 765,803 - mngA b0731 0.30 +0.6 765,803 - mngA b0731 0.30 +0.3 765,811 - mngA b0731 0.31 -0.1 765,811 - mngA b0731 0.31 +0.1 765,960 - mngA b0731 0.38 +0.1 766,059 - mngA b0731 0.43 +0.7 766,059 - mngA b0731 0.43 +0.7 766,066 - mngA b0731 0.43 +1.1 766,156 - mngA b0731 0.48 +0.4 766,156 - mngA b0731 0.48 +0.2 766,185 + mngA b0731 0.49 -0.1 766,263 + mngA b0731 0.53 +0.0 766,399 - mngA b0731 0.60 +0.4 766,767 + mngA b0731 0.79 -0.4 766,767 + mngA b0731 0.79 -0.1 766,848 + mngA b0731 0.83 +0.9 766,848 + mngA b0731 0.83 +0.5 767,053 + +0.5 767,053 + -0.1 767,053 + +0.3 767,158 + -0.1 767,158 + -0.3 767,226 - -0.3 767,257 + +0.2 767,309 + -0.1 767,329 + +0.1 767,329 + -0.7 767,473 - mngB b0732 0.10 -0.2 767,473 - mngB b0732 0.10 +0.1 767,473 - mngB b0732 0.10 +0.3 767,473 - mngB b0732 0.10 +0.2 767,535 - mngB b0732 0.13 +0.5 767,614 - mngB b0732 0.16 -0.1 767,614 - mngB b0732 0.16 -0.4 767,655 + mngB b0732 0.17 +0.4 767,717 - mngB b0732 0.20 -0.1 767,863 + mngB b0732 0.25 -0.0 767,917 + mngB b0732 0.27 +0.3 767,917 + mngB b0732 0.27 -0.2 767,917 + mngB b0732 0.27 -0.1 767,925 - mngB b0732 0.27 +0.5 767,937 - mngB b0732 0.28 +0.5 767,937 - mngB b0732 0.28 -0.1 768,026 + mngB b0732 0.31 -0.0 768,124 + mngB b0732 0.35 +0.3 768,131 + mngB b0732 0.35 +1.0 768,135 - mngB b0732 0.35 +0.6 768,135 - mngB b0732 0.35 +0.3
Or see this region's nucleotide sequence