Experiment: LB + carbenicillin regrowth after 48hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cydB and ybgT are separated by 14 nucleotides ybgT and ybgE overlap by 1 nucleotides ybgE and ybgC are separated by 149 nucleotides ybgC and tolQ overlap by 4 nucleotides
b0734: cydB - cytochrome d terminal oxidase, subunit II (NCBI), at 772,265 to 773,404
cydB
b4515: ybgT - hypothetical protein (NCBI), at 773,419 to 773,532
ybgT
b0735: ybgE - conserved inner membrane protein (NCBI), at 773,532 to 773,825
ybgE
b0736: ybgC - predicted acyl-CoA thioesterase (NCBI), at 773,975 to 774,379
ybgC
b0737: tolQ - membrane spanning protein in TolA-TolQ-TolR complex (NCBI), at 774,376 to 775,068
tolQ
Position (kb) 773
774 Strain fitness (log2 ratio) -1
0
1
2 at 773.380 kb on + strand at 773.486 kb on + strand, within ybgT at 773.572 kb on + strand, within ybgE at 773.617 kb on - strand, within ybgE at 773.693 kb on + strand, within ybgE at 773.693 kb on + strand, within ybgE at 773.702 kb on + strand, within ybgE at 773.702 kb on + strand, within ybgE at 773.857 kb on + strand at 773.857 kb on + strand at 773.900 kb on - strand at 773.900 kb on - strand at 773.968 kb on + strand at 773.974 kb on + strand at 773.974 kb on + strand at 774.007 kb on + strand at 774.007 kb on + strand at 774.103 kb on + strand, within ybgC at 774.103 kb on + strand, within ybgC at 774.256 kb on + strand, within ybgC at 774.265 kb on - strand, within ybgC at 774.299 kb on - strand, within ybgC at 774.336 kb on + strand, within ybgC at 774.390 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB + carbenicillin regrowth after 48hr remove 773,380 + -0.7 773,486 + ybgT b4515 0.59 +0.5 773,572 + ybgE b0735 0.14 +0.0 773,617 - ybgE b0735 0.29 +0.3 773,693 + ybgE b0735 0.55 +0.2 773,693 + ybgE b0735 0.55 +0.0 773,702 + ybgE b0735 0.58 +0.0 773,702 + ybgE b0735 0.58 +0.2 773,857 + -0.4 773,857 + +2.6 773,900 - -0.2 773,900 - -0.1 773,968 + +0.3 773,974 + +0.1 773,974 + -0.3 774,007 + -0.2 774,007 + -0.2 774,103 + ybgC b0736 0.32 -0.3 774,103 + ybgC b0736 0.32 +0.4 774,256 + ybgC b0736 0.69 +0.1 774,265 - ybgC b0736 0.72 +2.6 774,299 - ybgC b0736 0.80 -1.3 774,336 + ybgC b0736 0.89 +0.3 774,390 - +0.3
Or see this region's nucleotide sequence