Experiment: LB + carbenicillin regrowth after 48hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yaiN and yaiO are separated by 187 nucleotides yaiO and yaiX are separated by 1 nucleotides yaiX and insC are separated by 91 nucleotides insC and insD-1 overlap by 43 nucleotides
b0357: yaiN - putative alpha helix chain (VIMSS), at 378,830 to 379,105
yaiN
b0358: yaiO - hypothetical protein (NCBI), at 379,293 to 380,066
yaiO
b4579: yaiX - no description, at 380,068 to 380,483
yaiX
b0360: insC - KpLE2 phage-like element; IS2 insertion element repressor InsA (RefSeq), at 380,575 to 380,940
insC
b0361: insD-1 - IS2 insertion element transposase InsAB' (NCBI), at 380,898 to 381,803
insD-1
Position (kb) 380
381 Strain fitness (log2 ratio) -2
-1
0
1
2 at 379.409 kb on - strand, within yaiO at 379.409 kb on - strand, within yaiO at 379.439 kb on - strand, within yaiO at 379.480 kb on - strand, within yaiO at 379.481 kb on - strand, within yaiO at 379.573 kb on + strand, within yaiO at 379.573 kb on + strand, within yaiO at 379.580 kb on + strand, within yaiO at 379.616 kb on + strand, within yaiO at 379.616 kb on + strand, within yaiO at 379.616 kb on + strand, within yaiO at 379.616 kb on + strand, within yaiO at 379.616 kb on + strand, within yaiO at 379.616 kb on + strand, within yaiO at 379.616 kb on + strand, within yaiO at 379.616 kb on + strand, within yaiO at 379.618 kb on + strand, within yaiO at 379.618 kb on + strand, within yaiO at 379.621 kb on + strand, within yaiO at 379.621 kb on + strand, within yaiO at 379.621 kb on + strand, within yaiO at 379.621 kb on + strand, within yaiO at 379.621 kb on + strand, within yaiO at 379.621 kb on + strand, within yaiO at 379.625 kb on + strand, within yaiO at 379.625 kb on + strand, within yaiO at 379.628 kb on + strand, within yaiO at 379.628 kb on + strand, within yaiO at 379.629 kb on - strand, within yaiO at 379.629 kb on - strand, within yaiO at 379.629 kb on - strand, within yaiO at 379.629 kb on - strand, within yaiO at 379.629 kb on - strand, within yaiO at 379.629 kb on - strand, within yaiO at 379.770 kb on - strand, within yaiO at 379.779 kb on + strand, within yaiO at 379.779 kb on + strand, within yaiO at 379.787 kb on - strand, within yaiO at 379.787 kb on - strand, within yaiO at 379.788 kb on + strand, within yaiO at 379.798 kb on - strand, within yaiO at 379.798 kb on - strand, within yaiO at 379.814 kb on + strand, within yaiO at 379.825 kb on + strand, within yaiO at 379.831 kb on + strand, within yaiO at 379.831 kb on - strand, within yaiO at 379.833 kb on - strand, within yaiO at 379.833 kb on - strand, within yaiO at 379.833 kb on - strand, within yaiO at 379.833 kb on - strand, within yaiO at 379.833 kb on - strand, within yaiO at 379.837 kb on - strand, within yaiO at 379.848 kb on + strand, within yaiO at 379.848 kb on + strand, within yaiO at 379.928 kb on + strand, within yaiO at 379.928 kb on + strand, within yaiO at 379.928 kb on + strand, within yaiO at 379.928 kb on + strand, within yaiO at 379.936 kb on - strand, within yaiO at 379.988 kb on - strand, within yaiO at 379.993 kb on + strand at 379.993 kb on + strand at 379.995 kb on + strand at 380.009 kb on + strand at 380.088 kb on + strand at 380.088 kb on + strand at 380.128 kb on + strand, within yaiX at 380.130 kb on + strand, within yaiX at 380.136 kb on - strand, within yaiX at 380.143 kb on - strand, within yaiX at 380.186 kb on + strand, within yaiX at 380.208 kb on - strand, within yaiX at 380.213 kb on - strand, within yaiX at 380.215 kb on + strand, within yaiX at 380.215 kb on + strand, within yaiX at 380.215 kb on - strand, within yaiX at 380.217 kb on + strand, within yaiX at 380.217 kb on + strand, within yaiX at 380.227 kb on - strand, within yaiX at 380.269 kb on + strand, within yaiX at 380.270 kb on - strand, within yaiX at 380.283 kb on - strand, within yaiX at 380.283 kb on - strand, within yaiX at 380.287 kb on - strand, within yaiX at 380.295 kb on - strand, within yaiX at 380.306 kb on + strand, within yaiX at 380.314 kb on - strand, within yaiX at 380.316 kb on + strand, within yaiX at 380.316 kb on - strand, within yaiX at 380.320 kb on - strand, within yaiX at 380.322 kb on - strand, within yaiX at 380.322 kb on - strand, within yaiX at 380.331 kb on + strand, within yaiX at 380.357 kb on - strand, within yaiX at 380.357 kb on - strand, within yaiX at 380.369 kb on - strand, within yaiX at 380.387 kb on + strand, within yaiX at 380.449 kb on - strand at 380.488 kb on - strand at 380.488 kb on - strand at 380.488 kb on - strand at 380.500 kb on - strand at 380.500 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB + carbenicillin regrowth after 48hr remove 379,409 - yaiO b0358 0.15 -0.3 379,409 - yaiO b0358 0.15 -0.4 379,439 - yaiO b0358 0.19 +0.2 379,480 - yaiO b0358 0.24 +0.6 379,481 - yaiO b0358 0.24 +1.4 379,573 + yaiO b0358 0.36 -0.2 379,573 + yaiO b0358 0.36 +0.3 379,580 + yaiO b0358 0.37 +0.2 379,616 + yaiO b0358 0.42 +0.4 379,616 + yaiO b0358 0.42 -0.2 379,616 + yaiO b0358 0.42 +0.7 379,616 + yaiO b0358 0.42 -0.1 379,616 + yaiO b0358 0.42 +1.0 379,616 + yaiO b0358 0.42 +0.2 379,616 + yaiO b0358 0.42 -0.8 379,616 + yaiO b0358 0.42 +0.6 379,618 + yaiO b0358 0.42 -0.1 379,618 + yaiO b0358 0.42 +0.5 379,621 + yaiO b0358 0.42 +0.0 379,621 + yaiO b0358 0.42 -0.3 379,621 + yaiO b0358 0.42 -0.3 379,621 + yaiO b0358 0.42 -0.0 379,621 + yaiO b0358 0.42 +0.3 379,621 + yaiO b0358 0.42 -0.6 379,625 + yaiO b0358 0.43 +0.2 379,625 + yaiO b0358 0.43 +0.3 379,628 + yaiO b0358 0.43 +0.5 379,628 + yaiO b0358 0.43 +0.7 379,629 - yaiO b0358 0.43 +0.1 379,629 - yaiO b0358 0.43 +0.1 379,629 - yaiO b0358 0.43 +1.0 379,629 - yaiO b0358 0.43 -0.2 379,629 - yaiO b0358 0.43 -0.4 379,629 - yaiO b0358 0.43 +0.6 379,770 - yaiO b0358 0.62 -0.5 379,779 + yaiO b0358 0.63 +0.3 379,779 + yaiO b0358 0.63 -0.2 379,787 - yaiO b0358 0.64 -1.2 379,787 - yaiO b0358 0.64 +0.4 379,788 + yaiO b0358 0.64 +0.1 379,798 - yaiO b0358 0.65 -0.1 379,798 - yaiO b0358 0.65 +0.1 379,814 + yaiO b0358 0.67 -0.1 379,825 + yaiO b0358 0.69 -0.1 379,831 + yaiO b0358 0.70 +0.4 379,831 - yaiO b0358 0.70 +0.3 379,833 - yaiO b0358 0.70 -0.4 379,833 - yaiO b0358 0.70 +0.0 379,833 - yaiO b0358 0.70 -0.0 379,833 - yaiO b0358 0.70 +0.3 379,833 - yaiO b0358 0.70 -0.2 379,837 - yaiO b0358 0.70 +2.0 379,848 + yaiO b0358 0.72 -0.4 379,848 + yaiO b0358 0.72 +1.0 379,928 + yaiO b0358 0.82 +0.8 379,928 + yaiO b0358 0.82 -0.2 379,928 + yaiO b0358 0.82 +0.1 379,928 + yaiO b0358 0.82 -0.2 379,936 - yaiO b0358 0.83 +2.2 379,988 - yaiO b0358 0.90 +0.4 379,993 + -1.6 379,993 + -0.4 379,995 + +0.4 380,009 + +0.9 380,088 + +1.0 380,088 + -0.0 380,128 + yaiX b4579 0.14 +2.0 380,130 + yaiX b4579 0.15 +0.7 380,136 - yaiX b4579 0.16 +2.0 380,143 - yaiX b4579 0.18 +0.9 380,186 + yaiX b4579 0.28 +0.4 380,208 - yaiX b4579 0.34 +0.7 380,213 - yaiX b4579 0.35 +0.8 380,215 + yaiX b4579 0.35 +1.4 380,215 + yaiX b4579 0.35 +0.7 380,215 - yaiX b4579 0.35 +0.9 380,217 + yaiX b4579 0.36 +1.0 380,217 + yaiX b4579 0.36 +0.9 380,227 - yaiX b4579 0.38 +0.3 380,269 + yaiX b4579 0.48 +1.4 380,270 - yaiX b4579 0.49 +0.3 380,283 - yaiX b4579 0.52 -0.3 380,283 - yaiX b4579 0.52 -0.6 380,287 - yaiX b4579 0.53 +0.1 380,295 - yaiX b4579 0.55 +0.4 380,306 + yaiX b4579 0.57 +0.5 380,314 - yaiX b4579 0.59 -0.2 380,316 + yaiX b4579 0.60 +1.5 380,316 - yaiX b4579 0.60 -0.8 380,320 - yaiX b4579 0.61 +0.5 380,322 - yaiX b4579 0.61 -2.2 380,322 - yaiX b4579 0.61 +1.0 380,331 + yaiX b4579 0.63 +0.2 380,357 - yaiX b4579 0.69 +1.0 380,357 - yaiX b4579 0.69 -0.6 380,369 - yaiX b4579 0.72 +0.5 380,387 + yaiX b4579 0.77 -0.5 380,449 - +0.2 380,488 - -0.4 380,488 - -0.0 380,488 - +1.3 380,500 - +0.1 380,500 - +0.2
Or see this region's nucleotide sequence