Strain Fitness in Escherichia coli BW25113 around b3640

Experiment: LB + carbenicillin regrowth after 48hr

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntdfp and dut overlap by 20 nucleotidesdut and ttk are separated by 106 nucleotidesttk and pyrE are separated by 36 nucleotides b3639: dfp - flavoprotein affecting synthesis of DNA and pantothenate metabolism (VIMSS), at 3,810,754 to 3,811,974 dfp b3640: dut - deoxyuridine 5'-triphosphate nucleotidohydrolase (NCBI), at 3,811,955 to 3,812,410 dut b3641: ttk - putative transcriptional regulator (VIMSS), at 3,812,517 to 3,813,113 ttk b3642: pyrE - orotate phosphoribosyltransferase (NCBI), at 3,813,150 to 3,813,791 pyrE Position (kb) 3811 3812 3813Strain fitness (log2 ratio) -2 -1 0 1at 3812.407 kb on + strandat 3812.413 kb on - strandat 3812.413 kb on - strandat 3812.424 kb on + strandat 3812.567 kb on + strandat 3812.567 kb on + strandat 3812.604 kb on - strand, within ttkat 3812.662 kb on + strand, within ttkat 3812.662 kb on + strand, within ttkat 3812.700 kb on - strand, within ttkat 3812.700 kb on - strand, within ttkat 3812.759 kb on + strand, within ttkat 3812.759 kb on + strand, within ttkat 3812.816 kb on + strand, within ttkat 3812.821 kb on + strand, within ttkat 3812.824 kb on - strand, within ttkat 3812.860 kb on + strandat 3812.873 kb on - strand, within ttkat 3812.883 kb on - strand, within ttkat 3812.883 kb on - strand, within ttkat 3812.957 kb on + strand, within ttkat 3812.963 kb on + strand, within ttkat 3812.963 kb on + strand, within ttkat 3812.971 kb on - strand, within ttkat 3812.984 kb on - strand, within ttkat 3812.984 kb on - strand, within ttkat 3813.018 kb on + strand, within ttkat 3813.018 kb on + strand, within ttkat 3813.018 kb on + strand, within ttkat 3813.054 kb on - strandat 3813.168 kb on + strandat 3813.172 kb on - strandat 3813.175 kb on + strandat 3813.183 kb on - strandat 3813.183 kb on - strandat 3813.183 kb on - strandat 3813.236 kb on + strand, within pyrEat 3813.305 kb on + strand, within pyrEat 3813.320 kb on + strand, within pyrEat 3813.328 kb on - strand, within pyrE

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin regrowth after 48hr
remove
3,812,407 + -1.9
3,812,413 - +0.7
3,812,413 - -0.2
3,812,424 + -1.1
3,812,567 + +0.6
3,812,567 + +0.5
3,812,604 - ttk b3641 0.15 +0.4
3,812,662 + ttk b3641 0.24 +0.4
3,812,662 + ttk b3641 0.24 +0.3
3,812,700 - ttk b3641 0.31 +0.1
3,812,700 - ttk b3641 0.31 +0.3
3,812,759 + ttk b3641 0.41 +0.4
3,812,759 + ttk b3641 0.41 +0.2
3,812,816 + ttk b3641 0.50 +0.2
3,812,821 + ttk b3641 0.51 +0.1
3,812,824 - ttk b3641 0.51 +0.3
3,812,860 + +0.4
3,812,873 - ttk b3641 0.60 +0.1
3,812,883 - ttk b3641 0.61 -0.3
3,812,883 - ttk b3641 0.61 -0.5
3,812,957 + ttk b3641 0.74 +0.1
3,812,963 + ttk b3641 0.75 -0.2
3,812,963 + ttk b3641 0.75 +0.6
3,812,971 - ttk b3641 0.76 +0.5
3,812,984 - ttk b3641 0.78 +0.2
3,812,984 - ttk b3641 0.78 +0.6
3,813,018 + ttk b3641 0.84 +0.5
3,813,018 + ttk b3641 0.84 +0.6
3,813,018 + ttk b3641 0.84 +0.5
3,813,054 - +0.3
3,813,168 + +0.4
3,813,172 - +0.2
3,813,175 + +0.7
3,813,183 - -0.1
3,813,183 - -0.1
3,813,183 - +0.6
3,813,236 + pyrE b3642 0.13 -0.2
3,813,305 + pyrE b3642 0.24 +0.0
3,813,320 + pyrE b3642 0.26 -0.5
3,813,328 - pyrE b3642 0.28 +0.0

Or see this region's nucleotide sequence