Strain Fitness in Escherichia coli BW25113 around b2995

Experiment: LB + carbenicillin regrowth after 48hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthybC and hybB overlap by 4 nucleotideshybB and hybA overlap by 11 nucleotideshybA and hybO are separated by 2 nucleotides b2994: hybC - hydrogenase 2, large subunit (NCBI), at 3,139,308 to 3,141,011 hybC b2995: hybB - predicted hydrogenase 2 cytochrome b type component (NCBI), at 3,141,008 to 3,142,186 hybB b2996: hybA - hydrogenase 2 4Fe-4S ferredoxin-type component (NCBI), at 3,142,176 to 3,143,162 hybA b2997: hybO - hydrogenase 2, small subunit (NCBI), at 3,143,165 to 3,144,283 hybO Position (kb) 3141 3142 3143Strain fitness (log2 ratio) -1 0 1at 3140.013 kb on - strand, within hybCat 3140.013 kb on - strand, within hybCat 3140.129 kb on + strand, within hybCat 3140.159 kb on + strand, within hybCat 3140.167 kb on - strand, within hybCat 3140.167 kb on - strand, within hybCat 3140.227 kb on + strandat 3140.227 kb on + strand, within hybCat 3140.235 kb on + strand, within hybCat 3140.284 kb on + strand, within hybCat 3140.320 kb on - strand, within hybCat 3140.361 kb on + strand, within hybCat 3140.391 kb on - strand, within hybCat 3140.418 kb on + strand, within hybCat 3140.418 kb on + strand, within hybCat 3140.525 kb on - strand, within hybCat 3140.583 kb on + strand, within hybCat 3140.583 kb on + strand, within hybCat 3140.583 kb on + strand, within hybCat 3140.583 kb on + strand, within hybCat 3140.591 kb on - strand, within hybCat 3140.591 kb on - strand, within hybCat 3140.641 kb on - strand, within hybCat 3140.657 kb on + strand, within hybCat 3140.657 kb on + strand, within hybCat 3140.823 kb on - strand, within hybCat 3140.838 kb on + strand, within hybCat 3140.838 kb on + strand, within hybCat 3140.852 kb on - strandat 3140.885 kb on + strandat 3141.123 kb on + strandat 3141.192 kb on - strand, within hybBat 3141.215 kb on + strand, within hybBat 3141.215 kb on + strand, within hybBat 3141.223 kb on - strand, within hybBat 3141.240 kb on - strand, within hybBat 3141.240 kb on - strand, within hybBat 3141.286 kb on - strand, within hybBat 3141.341 kb on - strand, within hybBat 3141.341 kb on - strand, within hybBat 3141.472 kb on + strand, within hybBat 3141.486 kb on + strand, within hybBat 3141.554 kb on - strand, within hybBat 3141.632 kb on + strand, within hybBat 3141.642 kb on + strand, within hybBat 3141.642 kb on + strand, within hybBat 3141.657 kb on - strand, within hybBat 3141.676 kb on - strand, within hybBat 3141.733 kb on - strand, within hybBat 3141.738 kb on - strand, within hybBat 3141.738 kb on - strand, within hybBat 3141.751 kb on - strand, within hybBat 3141.751 kb on - strand, within hybBat 3141.779 kb on - strand, within hybBat 3141.804 kb on - strand, within hybBat 3141.808 kb on + strand, within hybBat 3141.843 kb on + strand, within hybBat 3141.851 kb on - strand, within hybBat 3141.888 kb on + strand, within hybBat 3141.901 kb on - strand, within hybBat 3141.901 kb on - strand, within hybBat 3141.913 kb on - strand, within hybBat 3141.955 kb on - strand, within hybBat 3141.955 kb on - strand, within hybBat 3142.110 kb on - strandat 3142.110 kb on - strandat 3142.271 kb on - strandat 3142.284 kb on - strand, within hybAat 3142.284 kb on - strand, within hybAat 3142.284 kb on - strand, within hybAat 3142.286 kb on - strand, within hybAat 3142.286 kb on - strand, within hybAat 3142.368 kb on + strand, within hybAat 3142.410 kb on + strand, within hybAat 3142.446 kb on - strand, within hybAat 3142.585 kb on - strand, within hybAat 3142.585 kb on - strand, within hybAat 3142.587 kb on + strand, within hybAat 3142.600 kb on + strand, within hybAat 3142.676 kb on - strand, within hybAat 3142.719 kb on - strand, within hybAat 3142.719 kb on - strand, within hybAat 3142.865 kb on + strand, within hybAat 3142.877 kb on + strand, within hybAat 3142.885 kb on - strand, within hybAat 3142.995 kb on - strand, within hybAat 3142.995 kb on - strand, within hybAat 3143.008 kb on + strand, within hybAat 3143.108 kb on - strandat 3143.108 kb on - strandat 3143.127 kb on + strandat 3143.127 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin regrowth after 48hr
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3,140,013 - hybC b2994 0.41 +0.2
3,140,013 - hybC b2994 0.41 +1.6
3,140,129 + hybC b2994 0.48 +0.2
3,140,159 + hybC b2994 0.50 +0.1
3,140,167 - hybC b2994 0.50 -0.2
3,140,167 - hybC b2994 0.50 +0.1
3,140,227 + +1.1
3,140,227 + hybC b2994 0.54 +1.2
3,140,235 + hybC b2994 0.54 +0.4
3,140,284 + hybC b2994 0.57 +0.1
3,140,320 - hybC b2994 0.59 -0.4
3,140,361 + hybC b2994 0.62 +0.3
3,140,391 - hybC b2994 0.64 +0.3
3,140,418 + hybC b2994 0.65 +0.2
3,140,418 + hybC b2994 0.65 +0.0
3,140,525 - hybC b2994 0.71 +0.2
3,140,583 + hybC b2994 0.75 +0.3
3,140,583 + hybC b2994 0.75 -0.3
3,140,583 + hybC b2994 0.75 -0.1
3,140,583 + hybC b2994 0.75 +0.5
3,140,591 - hybC b2994 0.75 +0.5
3,140,591 - hybC b2994 0.75 +0.4
3,140,641 - hybC b2994 0.78 +0.4
3,140,657 + hybC b2994 0.79 +0.3
3,140,657 + hybC b2994 0.79 +0.9
3,140,823 - hybC b2994 0.89 +0.3
3,140,838 + hybC b2994 0.90 +0.5
3,140,838 + hybC b2994 0.90 +0.8
3,140,852 - +0.0
3,140,885 + +0.5
3,141,123 + +0.4
3,141,192 - hybB b2995 0.16 +1.0
3,141,215 + hybB b2995 0.18 +0.3
3,141,215 + hybB b2995 0.18 +0.7
3,141,223 - hybB b2995 0.18 +0.1
3,141,240 - hybB b2995 0.20 +0.2
3,141,240 - hybB b2995 0.20 +0.4
3,141,286 - hybB b2995 0.24 +0.2
3,141,341 - hybB b2995 0.28 +0.1
3,141,341 - hybB b2995 0.28 -0.0
3,141,472 + hybB b2995 0.39 +1.1
3,141,486 + hybB b2995 0.41 +0.7
3,141,554 - hybB b2995 0.46 +0.6
3,141,632 + hybB b2995 0.53 +0.1
3,141,642 + hybB b2995 0.54 +0.5
3,141,642 + hybB b2995 0.54 +0.3
3,141,657 - hybB b2995 0.55 +0.3
3,141,676 - hybB b2995 0.57 +0.2
3,141,733 - hybB b2995 0.61 +0.0
3,141,738 - hybB b2995 0.62 +0.2
3,141,738 - hybB b2995 0.62 +0.7
3,141,751 - hybB b2995 0.63 +0.3
3,141,751 - hybB b2995 0.63 +0.7
3,141,779 - hybB b2995 0.65 +0.6
3,141,804 - hybB b2995 0.68 +0.4
3,141,808 + hybB b2995 0.68 +0.4
3,141,843 + hybB b2995 0.71 -0.1
3,141,851 - hybB b2995 0.72 +0.2
3,141,888 + hybB b2995 0.75 +0.4
3,141,901 - hybB b2995 0.76 +0.7
3,141,901 - hybB b2995 0.76 +0.6
3,141,913 - hybB b2995 0.77 +0.8
3,141,955 - hybB b2995 0.80 -0.2
3,141,955 - hybB b2995 0.80 +0.3
3,142,110 - +0.1
3,142,110 - +0.0
3,142,271 - +0.2
3,142,284 - hybA b2996 0.11 +0.1
3,142,284 - hybA b2996 0.11 +1.1
3,142,284 - hybA b2996 0.11 +0.0
3,142,286 - hybA b2996 0.11 +1.0
3,142,286 - hybA b2996 0.11 +0.4
3,142,368 + hybA b2996 0.19 +0.4
3,142,410 + hybA b2996 0.24 +0.8
3,142,446 - hybA b2996 0.27 +0.3
3,142,585 - hybA b2996 0.41 +0.3
3,142,585 - hybA b2996 0.41 +0.4
3,142,587 + hybA b2996 0.42 +0.5
3,142,600 + hybA b2996 0.43 +0.1
3,142,676 - hybA b2996 0.51 +0.2
3,142,719 - hybA b2996 0.55 -0.0
3,142,719 - hybA b2996 0.55 -0.0
3,142,865 + hybA b2996 0.70 +0.4
3,142,877 + hybA b2996 0.71 +0.2
3,142,885 - hybA b2996 0.72 +0.1
3,142,995 - hybA b2996 0.83 +1.1
3,142,995 - hybA b2996 0.83 -0.0
3,143,008 + hybA b2996 0.84 +0.5
3,143,108 - +0.0
3,143,108 - +1.2
3,143,127 + +0.7
3,143,127 + +0.3

Or see this region's nucleotide sequence