Strain Fitness in Escherichia coli BW25113 around b2488

Experiment: LB + carbenicillin regrowth after 48hr

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 nthyfG and hyfH are separated by 9 nucleotideshyfH and hyfI overlap by 4 nucleotideshyfI and b2490 overlap by 8 nucleotides b2487: hyfG - hydrogenase 4, subunit (NCBI), at 2,606,509 to 2,608,176 hyfG b2488: hyfH - hydrogenase 4, Fe-S subunit (NCBI), at 2,608,186 to 2,608,731 hyfH b2489: hyfI - hydrogenase 4, Fe-S subunit (NCBI), at 2,608,728 to 2,609,486 hyfI b2490: b2490 - putative protein processing element (VIMSS), at 2,609,479 to 2,609,892 b2490 Position (kb) 2608 2609Strain fitness (log2 ratio) -1 0 1at 2607.199 kb on + strand, within hyfGat 2607.250 kb on + strand, within hyfGat 2607.250 kb on + strand, within hyfGat 2607.258 kb on + strand, within hyfGat 2607.260 kb on + strand, within hyfGat 2607.264 kb on + strand, within hyfGat 2607.272 kb on - strand, within hyfGat 2607.354 kb on + strand, within hyfGat 2607.362 kb on + strand, within hyfGat 2607.397 kb on - strand, within hyfGat 2607.548 kb on + strand, within hyfGat 2607.838 kb on - strand, within hyfGat 2607.879 kb on + strand, within hyfGat 2608.003 kb on - strand, within hyfGat 2608.030 kb on - strandat 2608.070 kb on - strandat 2608.089 kb on - strandat 2608.121 kb on + strandat 2608.121 kb on + strandat 2608.130 kb on - strandat 2608.223 kb on - strandat 2608.416 kb on - strand, within hyfHat 2608.416 kb on - strand, within hyfHat 2608.658 kb on - strand, within hyfHat 2608.658 kb on - strand, within hyfHat 2608.733 kb on - strandat 2608.733 kb on - strandat 2608.757 kb on - strandat 2609.013 kb on + strand, within hyfIat 2609.013 kb on + strand, within hyfIat 2609.095 kb on - strand, within hyfIat 2609.097 kb on + strand, within hyfIat 2609.290 kb on - strand, within hyfIat 2609.329 kb on + strand, within hyfIat 2609.329 kb on + strand, within hyfIat 2609.393 kb on - strand, within hyfIat 2609.393 kb on - strand, within hyfIat 2609.480 kb on + strandat 2609.482 kb on + strandat 2609.490 kb on - strandat 2609.490 kb on - strandat 2609.576 kb on - strand, within b2490at 2609.576 kb on - strand, within b2490at 2609.595 kb on - strand, within b2490at 2609.671 kb on - strand, within b2490

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin regrowth after 48hr
remove
2,607,199 + hyfG b2487 0.41 +0.7
2,607,250 + hyfG b2487 0.44 +0.5
2,607,250 + hyfG b2487 0.44 -0.2
2,607,258 + hyfG b2487 0.45 -0.1
2,607,260 + hyfG b2487 0.45 +0.2
2,607,264 + hyfG b2487 0.45 +0.5
2,607,272 - hyfG b2487 0.46 +0.5
2,607,354 + hyfG b2487 0.51 -0.1
2,607,362 + hyfG b2487 0.51 -0.3
2,607,397 - hyfG b2487 0.53 +0.9
2,607,548 + hyfG b2487 0.62 +0.7
2,607,838 - hyfG b2487 0.80 -0.4
2,607,879 + hyfG b2487 0.82 -0.1
2,608,003 - hyfG b2487 0.90 +0.2
2,608,030 - +0.2
2,608,070 - +0.5
2,608,089 - +0.5
2,608,121 + +0.1
2,608,121 + -0.1
2,608,130 - -0.1
2,608,223 - +0.1
2,608,416 - hyfH b2488 0.42 +0.4
2,608,416 - hyfH b2488 0.42 +0.9
2,608,658 - hyfH b2488 0.86 +0.2
2,608,658 - hyfH b2488 0.86 +0.0
2,608,733 - +1.4
2,608,733 - +1.4
2,608,757 - +0.1
2,609,013 + hyfI b2489 0.38 +0.8
2,609,013 + hyfI b2489 0.38 -0.0
2,609,095 - hyfI b2489 0.48 +0.1
2,609,097 + hyfI b2489 0.49 +0.3
2,609,290 - hyfI b2489 0.74 +0.3
2,609,329 + hyfI b2489 0.79 -0.4
2,609,329 + hyfI b2489 0.79 -0.1
2,609,393 - hyfI b2489 0.88 +0.6
2,609,393 - hyfI b2489 0.88 +0.0
2,609,480 + -0.2
2,609,482 + -0.1
2,609,490 - -0.2
2,609,490 - +0.1
2,609,576 - b2490 b2490 0.23 +0.1
2,609,576 - b2490 b2490 0.23 +0.1
2,609,595 - b2490 b2490 0.28 +0.3
2,609,671 - b2490 b2490 0.46 +0.3

Or see this region's nucleotide sequence