Strain Fitness in Escherichia coli BW25113 around b2235

Experiment: LB + carbenicillin regrowth after 48hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnrdA and nrdB are separated by 233 nucleotidesnrdB and yfaE overlap by 1 nucleotidesyfaE and inaA are separated by 53 nucleotides b2234: nrdA - ribonucleotide-diphosphate reductase alpha subunit (NCBI), at 2,342,887 to 2,345,172 nrdA b2235: nrdB - ribonucleotide-diphosphate reductase beta subunit (NCBI), at 2,345,406 to 2,346,536 nrdB b2236: yfaE - predicted 2Fe-2S cluster-containing protein (NCBI), at 2,346,536 to 2,346,790 yfaE b2237: inaA - hypothetical protein (NCBI), at 2,346,844 to 2,347,494 inaA Position (kb) 2345 2346 2347Strain fitness (log2 ratio) -1 0 1at 2345.199 kb on + strandat 2346.543 kb on + strandat 2346.585 kb on - strand, within yfaEat 2346.626 kb on - strand, within yfaEat 2346.721 kb on - strand, within yfaEat 2346.851 kb on + strandat 2346.976 kb on - strand, within inaAat 2347.083 kb on + strand, within inaAat 2347.130 kb on + strand, within inaAat 2347.184 kb on + strand, within inaAat 2347.346 kb on + strand, within inaAat 2347.361 kb on - strand, within inaA

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin regrowth after 48hr
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2,345,199 + +0.4
2,346,543 + -0.0
2,346,585 - yfaE b2236 0.19 -0.0
2,346,626 - yfaE b2236 0.35 +0.1
2,346,721 - yfaE b2236 0.73 +0.0
2,346,851 + +0.9
2,346,976 - inaA b2237 0.20 -0.8
2,347,083 + inaA b2237 0.37 -0.2
2,347,130 + inaA b2237 0.44 +0.1
2,347,184 + inaA b2237 0.52 -0.8
2,347,346 + inaA b2237 0.77 +0.6
2,347,361 - inaA b2237 0.79 +0.2

Or see this region's nucleotide sequence