Strain Fitness in Escherichia coli BW25113 around b2234

Experiment: LB + carbenicillin regrowth after 48hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyfaL and nrdA are separated by 695 nucleotidesnrdA and nrdB are separated by 233 nucleotides b2233: yfaL - adhesin (NCBI), at 2,338,439 to 2,342,191 yfaL b2234: nrdA - ribonucleotide-diphosphate reductase alpha subunit (NCBI), at 2,342,887 to 2,345,172 nrdA b2235: nrdB - ribonucleotide-diphosphate reductase beta subunit (NCBI), at 2,345,406 to 2,346,536 nrdB Position (kb) 2342 2343 2344 2345 2346Strain fitness (log2 ratio) -1 0 1 2at 2342.011 kb on - strandat 2342.024 kb on - strandat 2342.077 kb on - strandat 2342.085 kb on + strandat 2342.096 kb on + strandat 2342.107 kb on + strandat 2342.114 kb on + strandat 2342.499 kb on - strandat 2342.499 kb on - strandat 2342.499 kb on - strandat 2342.509 kb on - strandat 2342.527 kb on - strandat 2342.534 kb on - strandat 2342.534 kb on - strandat 2342.612 kb on - strandat 2342.679 kb on - strandat 2342.679 kb on - strandat 2342.695 kb on - strandat 2342.729 kb on + strandat 2342.769 kb on + strandat 2342.781 kb on + strandat 2345.199 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin regrowth after 48hr
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2,342,011 - +0.8
2,342,024 - +0.8
2,342,077 - +0.2
2,342,085 + +0.2
2,342,096 + -1.3
2,342,107 + +0.2
2,342,114 + +0.2
2,342,499 - -0.1
2,342,499 - -0.3
2,342,499 - +0.3
2,342,509 - -0.8
2,342,527 - +2.2
2,342,534 - +0.2
2,342,534 - +1.2
2,342,612 - +0.1
2,342,679 - +0.2
2,342,679 - -0.4
2,342,695 - +2.0
2,342,729 + +0.0
2,342,769 + -0.3
2,342,781 + +0.4
2,345,199 + +0.4

Or see this region's nucleotide sequence