Experiment: LB + carbenicillin regrowth after 48hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydcU and ydcV overlap by 11 nucleotides ydcV and ydcW are separated by 21 nucleotides ydcW and yncL are separated by 96 nucleotides yncL and b1445 are separated by 194 nucleotides b1445 and ydcY are separated by 85 nucleotides ydcY and ydcZ are separated by 0 nucleotides
b1442: ydcU - predicted spermidine/putrescine transporter subunit (NCBI), at 1,511,855 to 1,512,796
ydcU
b1443: ydcV - predicted spermidine/putrescine transporter subunit (NCBI), at 1,512,786 to 1,513,580
ydcV
b1444: ydcW - medium chain aldehyde dehydrogenase (NCBI), at 1,513,602 to 1,515,026
ydcW
b4598: yncL - hypothetical protein (RefSeq), at 1,515,123 to 1,515,218
yncL
b1445: b1445 - orf, hypothetical protein (VIMSS), at 1,515,413 to 1,515,586
b1445
b1446: ydcY - hypothetical protein (NCBI), at 1,515,672 to 1,515,905
ydcY
b1447: ydcZ - predicted inner membrane protein (NCBI), at 1,515,906 to 1,516,355
ydcZ
Position (kb)
1513
1514
1515
1516 Strain fitness (log2 ratio)
-1
0
1 at 1512.754 kb on - strand at 1512.754 kb on - strand at 1512.889 kb on - strand, within ydcV at 1512.964 kb on - strand, within ydcV at 1512.964 kb on - strand, within ydcV at 1513.150 kb on - strand, within ydcV at 1513.161 kb on - strand, within ydcV at 1513.161 kb on - strand, within ydcV at 1513.229 kb on + strand, within ydcV at 1513.229 kb on + strand, within ydcV at 1514.101 kb on + strand, within ydcW at 1514.310 kb on - strand, within ydcW at 1514.401 kb on + strand, within ydcW at 1514.401 kb on + strand, within ydcW at 1514.435 kb on + strand, within ydcW at 1514.503 kb on + strand, within ydcW at 1514.625 kb on + strand, within ydcW at 1514.706 kb on + strand, within ydcW at 1514.920 kb on + strand at 1515.090 kb on + strand at 1515.199 kb on - strand, within yncL at 1515.247 kb on + strand at 1515.249 kb on + strand at 1515.252 kb on - strand at 1515.257 kb on - strand at 1515.257 kb on - strand at 1515.259 kb on + strand at 1515.608 kb on + strand at 1515.670 kb on - strand at 1515.791 kb on + strand, within ydcY at 1515.839 kb on - strand, within ydcY at 1515.839 kb on - strand, within ydcY at 1515.892 kb on + strand at 1515.892 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB + carbenicillin regrowth after 48hr remove 1,512,754 - -0.6 1,512,754 - +0.2 1,512,889 - ydcV b1443 0.13 +0.1 1,512,964 - ydcV b1443 0.22 +0.2 1,512,964 - ydcV b1443 0.22 +0.1 1,513,150 - ydcV b1443 0.46 +0.2 1,513,161 - ydcV b1443 0.47 +0.5 1,513,161 - ydcV b1443 0.47 -0.3 1,513,229 + ydcV b1443 0.56 +0.5 1,513,229 + ydcV b1443 0.56 -0.1 1,514,101 + ydcW b1444 0.35 +0.1 1,514,310 - ydcW b1444 0.50 -0.1 1,514,401 + ydcW b1444 0.56 +0.3 1,514,401 + ydcW b1444 0.56 +0.1 1,514,435 + ydcW b1444 0.58 +0.7 1,514,503 + ydcW b1444 0.63 +0.1 1,514,625 + ydcW b1444 0.72 +0.5 1,514,706 + ydcW b1444 0.77 +0.0 1,514,920 + +0.1 1,515,090 + -0.1 1,515,199 - yncL b4598 0.79 +0.1 1,515,247 + +0.3 1,515,249 + -0.2 1,515,252 - +0.2 1,515,257 - +0.1 1,515,257 - +0.1 1,515,259 + +0.7 1,515,608 + -0.1 1,515,670 - +0.1 1,515,791 + ydcY b1446 0.51 +0.1 1,515,839 - ydcY b1446 0.71 +0.2 1,515,839 - ydcY b1446 0.71 +0.5 1,515,892 + +0.6 1,515,892 + +0.3
Or see this region's nucleotide sequence