Strain Fitness in Escherichia coli BW25113 around b1302

Experiment: LB + carbenicillin regrowth after 48hr

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntpuuB and puuE are separated by 37 nucleotidespuuE and pspF are separated by 119 nucleotides b1301: puuB - gamma-Glu-putrescine oxidase, FAD/NAD(P)-binding (NCBI), at 1,362,256 to 1,363,536 puuB b1302: puuE - GABA aminotransferase, PLP-dependent (NCBI), at 1,363,574 to 1,364,839 puuE b1303: pspF - psp operon transcriptional activator (VIMSS), at 1,364,959 to 1,365,936 pspF Position (kb) 1363 1364 1365Strain fitness (log2 ratio) -1 0 1at 1362.664 kb on + strand, within puuBat 1362.716 kb on - strand, within puuBat 1362.757 kb on + strand, within puuBat 1363.276 kb on + strand, within puuBat 1363.284 kb on - strand, within puuBat 1363.311 kb on + strand, within puuBat 1363.311 kb on + strand, within puuBat 1363.332 kb on - strand, within puuBat 1363.341 kb on - strand, within puuBat 1363.503 kb on + strandat 1363.631 kb on - strandat 1363.749 kb on - strand, within puuEat 1363.749 kb on - strand, within puuEat 1363.940 kb on - strand, within puuEat 1363.993 kb on + strand, within puuEat 1364.468 kb on + strand, within puuEat 1364.619 kb on + strand, within puuEat 1364.739 kb on - strandat 1364.887 kb on - strandat 1364.951 kb on - strandat 1364.994 kb on + strandat 1365.094 kb on - strand, within pspFat 1365.094 kb on - strand, within pspFat 1365.177 kb on + strand, within pspFat 1365.234 kb on - strand, within pspFat 1365.275 kb on + strand, within pspFat 1365.299 kb on - strand, within pspFat 1365.299 kb on - strand, within pspFat 1365.402 kb on + strand, within pspFat 1365.559 kb on - strand, within pspFat 1365.559 kb on - strand, within pspFat 1365.591 kb on + strand, within pspF

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin regrowth after 48hr
remove
1,362,664 + puuB b1301 0.32 -0.1
1,362,716 - puuB b1301 0.36 -0.1
1,362,757 + puuB b1301 0.39 +0.1
1,363,276 + puuB b1301 0.80 +0.1
1,363,284 - puuB b1301 0.80 +0.4
1,363,311 + puuB b1301 0.82 +0.4
1,363,311 + puuB b1301 0.82 +0.3
1,363,332 - puuB b1301 0.84 +0.2
1,363,341 - puuB b1301 0.85 +0.7
1,363,503 + +0.3
1,363,631 - +0.3
1,363,749 - puuE b1302 0.14 -0.3
1,363,749 - puuE b1302 0.14 -0.1
1,363,940 - puuE b1302 0.29 -0.0
1,363,993 + puuE b1302 0.33 +0.5
1,364,468 + puuE b1302 0.71 +0.2
1,364,619 + puuE b1302 0.83 +0.3
1,364,739 - -0.1
1,364,887 - +0.8
1,364,951 - +0.4
1,364,994 + +0.6
1,365,094 - pspF b1303 0.14 +0.8
1,365,094 - pspF b1303 0.14 +0.2
1,365,177 + pspF b1303 0.22 -0.1
1,365,234 - pspF b1303 0.28 +0.3
1,365,275 + pspF b1303 0.32 -0.5
1,365,299 - pspF b1303 0.35 +0.2
1,365,299 - pspF b1303 0.35 -0.4
1,365,402 + pspF b1303 0.45 -0.0
1,365,559 - pspF b1303 0.61 -0.6
1,365,559 - pspF b1303 0.61 +0.1
1,365,591 + pspF b1303 0.65 -0.2

Or see this region's nucleotide sequence