Strain Fitness in Escherichia coli BW25113 around b1227

Experiment: LB + carbenicillin regrowth after 48hr

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntnarH and narJ overlap by 4 nucleotidesnarJ and narI overlap by 1 nucleotidesnarI and rttR are separated by 539 nucleotidesrttR and tpr overlap by 150 nucleotidestpr and tyrV are separated by 67 nucleotides b1225: narH - nitrate reductase 1, beta (Fe-S) subunit (NCBI), at 1,282,827 to 1,284,365 narH b1226: narJ - molybdenum-cofactor-assembly chaperone subunit (delta subunit) of nitrate reductase 1 (NCBI), at 1,284,362 to 1,285,072 narJ b1227: narI - nitrate reductase 1, gamma (cytochrome b(NR)) subunit (NCBI), at 1,285,072 to 1,285,749 narI b4425: rttR - regulatory sRNA (NCBI), at 1,286,289 to 1,286,459 rttR b1229: tpr - predicted protamine-like protein (RefSeq), at 1,286,310 to 1,286,399 tpr b1230: tyrV - tRNA-Tyr (NCBI), at 1,286,467 to 1,286,551 tyrV Position (kb) 1285 1286Strain fitness (log2 ratio) -1 0 1at 1284.099 kb on + strand, within narHat 1284.152 kb on + strand, within narHat 1284.436 kb on + strand, within narJat 1284.436 kb on + strand, within narJat 1284.480 kb on + strand, within narJat 1284.554 kb on - strand, within narJat 1284.634 kb on + strand, within narJat 1284.728 kb on - strand, within narJat 1284.809 kb on + strand, within narJat 1284.809 kb on + strand, within narJat 1285.100 kb on + strandat 1285.167 kb on + strand, within narIat 1285.352 kb on - strand, within narIat 1285.352 kb on - strand, within narIat 1285.476 kb on + strand, within narIat 1285.509 kb on - strand, within narIat 1285.518 kb on + strand, within narIat 1285.518 kb on + strand, within narIat 1285.526 kb on - strand, within narIat 1285.605 kb on - strand, within narIat 1285.670 kb on - strand, within narIat 1285.686 kb on + strandat 1285.694 kb on - strandat 1285.707 kb on + strandat 1286.125 kb on + strandat 1286.316 kb on - strandat 1286.316 kb on - strandat 1286.690 kb on - strandat 1286.690 kb on - strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin regrowth after 48hr
remove
1,284,099 + narH b1225 0.83 +0.2
1,284,152 + narH b1225 0.86 -0.1
1,284,436 + narJ b1226 0.10 +0.3
1,284,436 + narJ b1226 0.10 +0.3
1,284,480 + narJ b1226 0.17 -0.3
1,284,554 - narJ b1226 0.27 +0.3
1,284,634 + narJ b1226 0.38 +0.0
1,284,728 - narJ b1226 0.51 +0.5
1,284,809 + narJ b1226 0.63 +0.3
1,284,809 + narJ b1226 0.63 +0.3
1,285,100 + +0.4
1,285,167 + narI b1227 0.14 +0.9
1,285,352 - narI b1227 0.41 +1.4
1,285,352 - narI b1227 0.41 +0.7
1,285,476 + narI b1227 0.60 +0.0
1,285,509 - narI b1227 0.64 +0.5
1,285,518 + narI b1227 0.66 -0.0
1,285,518 + narI b1227 0.66 +0.1
1,285,526 - narI b1227 0.67 +0.4
1,285,605 - narI b1227 0.79 +0.5
1,285,670 - narI b1227 0.88 -0.1
1,285,686 + -0.2
1,285,694 - -0.0
1,285,707 + +0.4
1,286,125 + +0.7
1,286,316 - +0.8
1,286,316 - +0.4
1,286,690 - -0.3
1,286,690 - +0.0

Or see this region's nucleotide sequence