Strain Fitness in Escherichia coli BW25113 around b1083

Experiment: LB + carbenicillin regrowth after 48hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntflgK and flgL are separated by 11 nucleotidesflgL and rne are separated by 195 nucleotides b1082: flgK - flagellar hook-associated protein K (NCBI), at 1,137,601 to 1,139,244 flgK b1083: flgL - flagellar hook-associated protein L (NCBI), at 1,139,256 to 1,140,209 flgL b1084: rne - fused ribonucleaseE: endoribonuclease/RNA-binding protein/RNA degradosome binding protein (NCBI), at 1,140,405 to 1,143,590 rne Position (kb) 1139 1140 1141Strain fitness (log2 ratio) -1 0 1at 1138.306 kb on + strand, within flgKat 1138.350 kb on + strand, within flgKat 1138.362 kb on + strand, within flgKat 1138.369 kb on + strand, within flgKat 1138.422 kb on + strand, within flgKat 1138.422 kb on + strand, within flgKat 1138.469 kb on + strand, within flgKat 1138.530 kb on - strand, within flgKat 1139.018 kb on - strand, within flgKat 1139.153 kb on - strandat 1139.172 kb on + strandat 1139.261 kb on + strandat 1139.261 kb on + strandat 1139.311 kb on - strandat 1139.311 kb on - strandat 1139.337 kb on + strandat 1139.413 kb on + strand, within flgLat 1139.413 kb on + strand, within flgLat 1139.498 kb on - strand, within flgLat 1139.498 kb on - strand, within flgLat 1139.555 kb on - strand, within flgLat 1139.710 kb on - strand, within flgLat 1139.734 kb on + strand, within flgLat 1139.977 kb on + strand, within flgLat 1140.119 kb on + strandat 1140.127 kb on - strandat 1140.127 kb on - strandat 1140.299 kb on - strandat 1140.390 kb on + strandat 1140.596 kb on + strandat 1140.597 kb on - strandat 1140.818 kb on + strand, within rneat 1140.908 kb on - strand, within rneat 1140.947 kb on + strand, within rneat 1141.001 kb on - strand, within rneat 1141.026 kb on - strand, within rneat 1141.067 kb on - strand, within rneat 1141.067 kb on - strand, within rneat 1141.150 kb on - strand, within rne

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin regrowth after 48hr
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1,138,306 + flgK b1082 0.43 +0.0
1,138,350 + flgK b1082 0.46 +0.1
1,138,362 + flgK b1082 0.46 +0.4
1,138,369 + flgK b1082 0.47 -0.0
1,138,422 + flgK b1082 0.50 +0.2
1,138,422 + flgK b1082 0.50 -0.5
1,138,469 + flgK b1082 0.53 +0.4
1,138,530 - flgK b1082 0.57 +0.2
1,139,018 - flgK b1082 0.86 +0.0
1,139,153 - +0.7
1,139,172 + +0.1
1,139,261 + -0.2
1,139,261 + +0.6
1,139,311 - +0.2
1,139,311 - +0.0
1,139,337 + +0.4
1,139,413 + flgL b1083 0.16 +0.3
1,139,413 + flgL b1083 0.16 +0.4
1,139,498 - flgL b1083 0.25 +0.5
1,139,498 - flgL b1083 0.25 +1.5
1,139,555 - flgL b1083 0.31 -0.0
1,139,710 - flgL b1083 0.48 +1.1
1,139,734 + flgL b1083 0.50 -0.3
1,139,977 + flgL b1083 0.76 +0.2
1,140,119 + +0.1
1,140,127 - +0.4
1,140,127 - -0.0
1,140,299 - +0.3
1,140,390 + +0.4
1,140,596 + -0.4
1,140,597 - -0.3
1,140,818 + rne b1084 0.13 +0.4
1,140,908 - rne b1084 0.16 -0.0
1,140,947 + rne b1084 0.17 -0.6
1,141,001 - rne b1084 0.19 +0.2
1,141,026 - rne b1084 0.19 +0.4
1,141,067 - rne b1084 0.21 -0.6
1,141,067 - rne b1084 0.21 -1.1
1,141,150 - rne b1084 0.23 -0.3

Or see this region's nucleotide sequence