Experiment: LB + carbenicillin regrowth after 48hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aroA and ycaL are separated by 168 nucleotides ycaL and cmk are separated by 172 nucleotides cmk and rpsA are separated by 110 nucleotides
b0908: aroA - 3-phosphoshikimate 1-carboxyvinyltransferase (NCBI), at 958,035 to 959,318
aroA
b0909: ycaL - putative heat shock protein (VIMSS), at 959,487 to 960,251
ycaL
b0910: cmk - cytidylate kinase (NCBI), at 960,424 to 961,107
cmk
b0911: rpsA - 30S ribosomal protein S1 (NCBI), at 961,218 to 962,891
rpsA
Position (kb) 959
960
961 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 958.501 kb on + strand, within aroA at 958.531 kb on - strand, within aroA at 958.531 kb on - strand, within aroA at 958.552 kb on + strand, within aroA at 958.552 kb on + strand, within aroA at 958.721 kb on + strand, within aroA at 958.728 kb on - strand, within aroA at 958.730 kb on - strand, within aroA at 958.738 kb on - strand, within aroA at 958.738 kb on - strand, within aroA at 958.794 kb on + strand, within aroA at 958.794 kb on + strand, within aroA at 958.810 kb on + strand, within aroA at 958.810 kb on + strand, within aroA at 958.825 kb on + strand, within aroA at 958.833 kb on - strand, within aroA at 958.833 kb on - strand, within aroA at 958.922 kb on - strand, within aroA at 958.922 kb on - strand, within aroA at 958.932 kb on + strand, within aroA at 958.932 kb on + strand, within aroA at 958.934 kb on + strand, within aroA at 958.942 kb on - strand, within aroA at 959.025 kb on - strand, within aroA at 959.034 kb on + strand, within aroA at 959.042 kb on - strand, within aroA at 959.042 kb on - strand, within aroA at 959.080 kb on + strand, within aroA at 959.299 kb on + strand at 959.304 kb on + strand at 959.529 kb on - strand at 959.542 kb on + strand at 959.551 kb on - strand at 959.551 kb on - strand at 959.560 kb on + strand at 959.613 kb on + strand, within ycaL at 959.621 kb on - strand, within ycaL at 959.621 kb on - strand, within ycaL at 959.629 kb on + strand, within ycaL at 959.661 kb on + strand, within ycaL at 959.792 kb on + strand, within ycaL at 959.800 kb on - strand, within ycaL at 959.911 kb on + strand, within ycaL at 960.118 kb on - strand, within ycaL at 960.152 kb on - strand, within ycaL at 960.170 kb on + strand, within ycaL at 960.197 kb on + strand at 960.200 kb on - strand at 960.223 kb on + strand at 960.312 kb on + strand at 960.312 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB + carbenicillin regrowth after 48hr remove 958,501 + aroA b0908 0.36 -1.7 958,531 - aroA b0908 0.39 -1.1 958,531 - aroA b0908 0.39 +0.1 958,552 + aroA b0908 0.40 +1.3 958,552 + aroA b0908 0.40 -2.0 958,721 + aroA b0908 0.53 -0.7 958,728 - aroA b0908 0.54 -1.4 958,730 - aroA b0908 0.54 -2.9 958,738 - aroA b0908 0.55 -1.3 958,738 - aroA b0908 0.55 -1.2 958,794 + aroA b0908 0.59 -0.3 958,794 + aroA b0908 0.59 -0.9 958,810 + aroA b0908 0.60 -0.9 958,810 + aroA b0908 0.60 -1.9 958,825 + aroA b0908 0.62 -1.4 958,833 - aroA b0908 0.62 -2.5 958,833 - aroA b0908 0.62 -1.6 958,922 - aroA b0908 0.69 -1.7 958,922 - aroA b0908 0.69 +0.3 958,932 + aroA b0908 0.70 -2.2 958,932 + aroA b0908 0.70 -0.4 958,934 + aroA b0908 0.70 -0.7 958,942 - aroA b0908 0.71 -1.0 959,025 - aroA b0908 0.77 -1.7 959,034 + aroA b0908 0.78 -1.6 959,042 - aroA b0908 0.78 -0.9 959,042 - aroA b0908 0.78 -1.9 959,080 + aroA b0908 0.81 -2.4 959,299 + +0.3 959,304 + +0.1 959,529 - +0.0 959,542 + -0.1 959,551 - -0.2 959,551 - +0.8 959,560 + +0.2 959,613 + ycaL b0909 0.16 +0.2 959,621 - ycaL b0909 0.18 +0.2 959,621 - ycaL b0909 0.18 +0.4 959,629 + ycaL b0909 0.19 +0.4 959,661 + ycaL b0909 0.23 +1.4 959,792 + ycaL b0909 0.40 +0.2 959,800 - ycaL b0909 0.41 +0.5 959,911 + ycaL b0909 0.55 +1.1 960,118 - ycaL b0909 0.82 -0.3 960,152 - ycaL b0909 0.87 +0.7 960,170 + ycaL b0909 0.89 +0.2 960,197 + +0.2 960,200 - +0.6 960,223 + -0.3 960,312 + +0.1 960,312 + -0.9
Or see this region's nucleotide sequence