Experiment: LB + carbenicillin regrowth after 48hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt djlB and ybeT are separated by 9 nucleotides ybeT and ybeU are separated by 101 nucleotides ybeU and djlC overlap by 4 nucleotides
b0646: djlB - predicted chaperone (NCBI), at 676,638 to 678,065
djlB
b0647: ybeT - conserved outer membrane protein (NCBI), at 678,075 to 678,629
ybeT
b0648: ybeU - predicted tRNA ligase (NCBI), at 678,731 to 679,438
ybeU
b0649: djlC - Hsc56 co-chaperone of HscC (NCBI), at 679,435 to 680,886
djlC
Position (kb) 678
679
680 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 677.737 kb on - strand, within djlB at 677.737 kb on - strand, within djlB at 677.741 kb on + strand, within djlB at 677.743 kb on + strand, within djlB at 677.746 kb on + strand, within djlB at 677.746 kb on + strand, within djlB at 677.746 kb on + strand, within djlB at 677.746 kb on + strand, within djlB at 677.746 kb on + strand, within djlB at 677.746 kb on + strand, within djlB at 677.751 kb on + strand, within djlB at 677.751 kb on + strand, within djlB at 677.766 kb on + strand, within djlB at 677.768 kb on + strand, within djlB at 677.768 kb on + strand, within djlB at 677.793 kb on + strand, within djlB at 677.793 kb on + strand, within djlB at 677.793 kb on + strand, within djlB at 677.793 kb on + strand, within djlB at 677.793 kb on + strand, within djlB at 677.801 kb on - strand, within djlB at 677.806 kb on - strand, within djlB at 677.806 kb on - strand, within djlB at 677.807 kb on + strand, within djlB at 677.807 kb on + strand, within djlB at 677.832 kb on + strand, within djlB at 677.948 kb on + strand at 677.951 kb on - strand at 677.956 kb on - strand at 677.961 kb on + strand at 677.961 kb on + strand at 677.967 kb on + strand at 677.971 kb on + strand at 677.971 kb on + strand at 677.971 kb on + strand at 677.976 kb on + strand at 677.976 kb on + strand at 677.977 kb on + strand at 677.977 kb on + strand at 677.978 kb on + strand at 677.981 kb on + strand at 677.981 kb on + strand at 677.986 kb on + strand at 677.986 kb on + strand at 677.986 kb on + strand at 677.986 kb on + strand at 678.033 kb on - strand at 678.041 kb on + strand at 678.054 kb on - strand at 678.054 kb on - strand at 678.066 kb on + strand at 678.080 kb on - strand at 678.170 kb on + strand, within ybeT at 678.216 kb on + strand, within ybeT at 678.231 kb on - strand, within ybeT at 678.236 kb on - strand, within ybeT at 678.239 kb on - strand, within ybeT at 678.245 kb on + strand, within ybeT at 678.249 kb on - strand, within ybeT at 678.257 kb on + strand, within ybeT at 678.270 kb on + strand, within ybeT at 678.271 kb on - strand, within ybeT at 678.271 kb on - strand, within ybeT at 678.280 kb on - strand, within ybeT at 678.280 kb on - strand, within ybeT at 678.280 kb on - strand, within ybeT at 678.284 kb on + strand, within ybeT at 678.284 kb on + strand, within ybeT at 678.285 kb on + strand, within ybeT at 678.285 kb on + strand, within ybeT at 678.292 kb on - strand, within ybeT at 678.292 kb on - strand, within ybeT at 678.299 kb on - strand, within ybeT at 678.302 kb on + strand at 678.306 kb on - strand, within ybeT at 678.306 kb on - strand, within ybeT at 678.306 kb on - strand, within ybeT at 678.313 kb on + strand, within ybeT at 678.353 kb on + strand, within ybeT at 678.353 kb on + strand, within ybeT at 678.364 kb on + strand, within ybeT at 678.368 kb on - strand, within ybeT at 678.382 kb on - strand, within ybeT at 678.388 kb on + strand, within ybeT at 678.388 kb on + strand, within ybeT at 678.397 kb on - strand, within ybeT at 678.432 kb on + strand, within ybeT at 678.432 kb on - strand, within ybeT at 678.432 kb on - strand, within ybeT at 678.442 kb on + strand, within ybeT at 678.447 kb on + strand, within ybeT at 678.452 kb on + strand, within ybeT at 678.464 kb on + strand, within ybeT at 678.465 kb on + strand, within ybeT at 678.465 kb on + strand, within ybeT at 678.469 kb on + strand, within ybeT at 678.475 kb on + strand, within ybeT at 678.475 kb on + strand, within ybeT at 678.481 kb on + strand, within ybeT at 678.481 kb on + strand, within ybeT at 678.485 kb on + strand, within ybeT at 678.485 kb on + strand, within ybeT at 678.485 kb on + strand, within ybeT at 678.485 kb on + strand, within ybeT at 678.485 kb on + strand, within ybeT at 678.543 kb on + strand, within ybeT at 678.566 kb on + strand, within ybeT at 678.801 kb on - strand at 678.907 kb on - strand, within ybeU at 678.917 kb on - strand, within ybeU at 678.946 kb on + strand, within ybeU at 678.949 kb on - strand, within ybeU at 678.964 kb on - strand, within ybeU at 678.966 kb on - strand, within ybeU at 678.966 kb on - strand, within ybeU at 678.966 kb on - strand, within ybeU at 678.966 kb on - strand, within ybeU at 678.966 kb on - strand, within ybeU at 678.969 kb on + strand, within ybeU at 678.977 kb on - strand, within ybeU at 678.977 kb on - strand, within ybeU at 678.977 kb on - strand, within ybeU at 678.977 kb on - strand, within ybeU at 679.009 kb on - strand, within ybeU at 679.014 kb on + strand, within ybeU at 679.045 kb on - strand, within ybeU at 679.051 kb on + strand, within ybeU at 679.058 kb on + strand, within ybeU at 679.059 kb on - strand, within ybeU at 679.059 kb on - strand, within ybeU at 679.072 kb on - strand, within ybeU at 679.113 kb on - strand, within ybeU at 679.121 kb on + strand, within ybeU at 679.243 kb on - strand, within ybeU at 679.293 kb on - strand, within ybeU at 679.293 kb on - strand, within ybeU at 679.350 kb on + strand, within ybeU at 679.381 kb on + strand at 679.414 kb on + strand at 679.414 kb on + strand at 679.415 kb on + strand at 679.415 kb on + strand at 679.538 kb on + strand at 679.547 kb on + strand at 679.547 kb on + strand at 679.547 kb on + strand at 679.547 kb on + strand at 679.569 kb on - strand at 679.571 kb on + strand at 679.574 kb on - strand at 679.576 kb on - strand at 679.785 kb on - strand, within djlC at 679.788 kb on + strand, within djlC at 679.828 kb on - strand, within djlC at 679.964 kb on - strand, within djlC at 679.964 kb on - strand, within djlC at 679.979 kb on - strand, within djlC at 680.012 kb on + strand, within djlC at 680.020 kb on - strand, within djlC at 680.034 kb on - strand, within djlC at 680.034 kb on - strand, within djlC at 680.136 kb on - strand, within djlC at 680.160 kb on - strand, within djlC at 680.196 kb on + strand, within djlC at 680.196 kb on + strand, within djlC at 680.234 kb on + strand, within djlC at 680.234 kb on + strand, within djlC
Per-strain Table
Position Strand Gene LocusTag Fraction LB + carbenicillin regrowth after 48hr remove 677,737 - djlB b0646 0.77 -2.0 677,737 - djlB b0646 0.77 +0.3 677,741 + djlB b0646 0.77 +0.0 677,743 + djlB b0646 0.77 -1.2 677,746 + djlB b0646 0.78 -0.2 677,746 + djlB b0646 0.78 -0.2 677,746 + djlB b0646 0.78 +0.1 677,746 + djlB b0646 0.78 +0.1 677,746 + djlB b0646 0.78 +0.4 677,746 + djlB b0646 0.78 +0.3 677,751 + djlB b0646 0.78 +0.4 677,751 + djlB b0646 0.78 +0.4 677,766 + djlB b0646 0.79 +0.4 677,768 + djlB b0646 0.79 -0.1 677,768 + djlB b0646 0.79 +0.3 677,793 + djlB b0646 0.81 -0.2 677,793 + djlB b0646 0.81 +0.3 677,793 + djlB b0646 0.81 -1.0 677,793 + djlB b0646 0.81 -0.6 677,793 + djlB b0646 0.81 +0.4 677,801 - djlB b0646 0.81 +0.3 677,806 - djlB b0646 0.82 +0.3 677,806 - djlB b0646 0.82 +0.3 677,807 + djlB b0646 0.82 +0.6 677,807 + djlB b0646 0.82 +0.2 677,832 + djlB b0646 0.84 +1.3 677,948 + +0.1 677,951 - +1.1 677,956 - +1.3 677,961 + +1.6 677,961 + -0.4 677,967 + -0.1 677,971 + -0.1 677,971 + +0.6 677,971 + +0.2 677,976 + -0.5 677,976 + +0.3 677,977 + +0.4 677,977 + -0.2 677,978 + +0.1 677,981 + -0.2 677,981 + +0.2 677,986 + +0.7 677,986 + +1.5 677,986 + +0.0 677,986 + -1.1 678,033 - -0.3 678,041 + -0.3 678,054 - +0.3 678,054 - +0.1 678,066 + +0.4 678,080 - +0.3 678,170 + ybeT b0647 0.17 +0.3 678,216 + ybeT b0647 0.25 +0.3 678,231 - ybeT b0647 0.28 +1.9 678,236 - ybeT b0647 0.29 +0.0 678,239 - ybeT b0647 0.30 +0.0 678,245 + ybeT b0647 0.31 +0.7 678,249 - ybeT b0647 0.31 +3.3 678,257 + ybeT b0647 0.33 -0.5 678,270 + ybeT b0647 0.35 -2.9 678,271 - ybeT b0647 0.35 +1.0 678,271 - ybeT b0647 0.35 +1.5 678,280 - ybeT b0647 0.37 +1.3 678,280 - ybeT b0647 0.37 -0.7 678,280 - ybeT b0647 0.37 +1.9 678,284 + ybeT b0647 0.38 -0.7 678,284 + ybeT b0647 0.38 -0.7 678,285 + ybeT b0647 0.38 +1.3 678,285 + ybeT b0647 0.38 +0.3 678,292 - ybeT b0647 0.39 -1.0 678,292 - ybeT b0647 0.39 -0.7 678,299 - ybeT b0647 0.40 +1.5 678,302 + +0.3 678,306 - ybeT b0647 0.42 -0.7 678,306 - ybeT b0647 0.42 -1.1 678,306 - ybeT b0647 0.42 -0.1 678,313 + ybeT b0647 0.43 +1.1 678,353 + ybeT b0647 0.50 +0.1 678,353 + ybeT b0647 0.50 +0.8 678,364 + ybeT b0647 0.52 +0.7 678,368 - ybeT b0647 0.53 -0.7 678,382 - ybeT b0647 0.55 +0.3 678,388 + ybeT b0647 0.56 +0.6 678,388 + ybeT b0647 0.56 -0.2 678,397 - ybeT b0647 0.58 +0.3 678,432 + ybeT b0647 0.64 +0.1 678,432 - ybeT b0647 0.64 +0.3 678,432 - ybeT b0647 0.64 +1.3 678,442 + ybeT b0647 0.66 -1.5 678,447 + ybeT b0647 0.67 +0.3 678,452 + ybeT b0647 0.68 +0.8 678,464 + ybeT b0647 0.70 +0.3 678,465 + ybeT b0647 0.70 +0.8 678,465 + ybeT b0647 0.70 -0.4 678,469 + ybeT b0647 0.71 -0.5 678,475 + ybeT b0647 0.72 -0.1 678,475 + ybeT b0647 0.72 +0.1 678,481 + ybeT b0647 0.73 +0.3 678,481 + ybeT b0647 0.73 -2.0 678,485 + ybeT b0647 0.74 -0.3 678,485 + ybeT b0647 0.74 +1.3 678,485 + ybeT b0647 0.74 -1.3 678,485 + ybeT b0647 0.74 +1.3 678,485 + ybeT b0647 0.74 +1.3 678,543 + ybeT b0647 0.84 -2.2 678,566 + ybeT b0647 0.88 +0.3 678,801 - +0.3 678,907 - ybeU b0648 0.25 -0.5 678,917 - ybeU b0648 0.26 +1.6 678,946 + ybeU b0648 0.30 +0.3 678,949 - ybeU b0648 0.31 +1.9 678,964 - ybeU b0648 0.33 +0.4 678,966 - ybeU b0648 0.33 +2.6 678,966 - ybeU b0648 0.33 +1.2 678,966 - ybeU b0648 0.33 +1.5 678,966 - ybeU b0648 0.33 +0.6 678,966 - ybeU b0648 0.33 +0.3 678,969 + ybeU b0648 0.34 +1.3 678,977 - ybeU b0648 0.35 +0.3 678,977 - ybeU b0648 0.35 -0.1 678,977 - ybeU b0648 0.35 +0.5 678,977 - ybeU b0648 0.35 +0.2 679,009 - ybeU b0648 0.39 +0.3 679,014 + ybeU b0648 0.40 +0.9 679,045 - ybeU b0648 0.44 +1.1 679,051 + ybeU b0648 0.45 -0.1 679,058 + ybeU b0648 0.46 +0.4 679,059 - ybeU b0648 0.46 +0.7 679,059 - ybeU b0648 0.46 +0.6 679,072 - ybeU b0648 0.48 +2.5 679,113 - ybeU b0648 0.54 -0.7 679,121 + ybeU b0648 0.55 +1.0 679,243 - ybeU b0648 0.72 +1.2 679,293 - ybeU b0648 0.79 +0.4 679,293 - ybeU b0648 0.79 +0.1 679,350 + ybeU b0648 0.87 +0.2 679,381 + -0.5 679,414 + -0.4 679,414 + +0.2 679,415 + +0.6 679,415 + -0.3 679,538 + +0.3 679,547 + +0.4 679,547 + +0.2 679,547 + -0.1 679,547 + -0.3 679,569 - +0.9 679,571 + +2.0 679,574 - +0.2 679,576 - +0.5 679,785 - djlC b0649 0.24 -0.1 679,788 + djlC b0649 0.24 -0.0 679,828 - djlC b0649 0.27 -0.5 679,964 - djlC b0649 0.36 -0.3 679,964 - djlC b0649 0.36 -1.0 679,979 - djlC b0649 0.37 -0.2 680,012 + djlC b0649 0.40 +0.3 680,020 - djlC b0649 0.40 -0.8 680,034 - djlC b0649 0.41 +0.6 680,034 - djlC b0649 0.41 +1.2 680,136 - djlC b0649 0.48 -0.0 680,160 - djlC b0649 0.50 +0.6 680,196 + djlC b0649 0.52 -0.1 680,196 + djlC b0649 0.52 +0.3 680,234 + djlC b0649 0.55 -0.3 680,234 + djlC b0649 0.55 -0.3
Or see this region's nucleotide sequence