Experiment: LB + ciprofloxacin Time48hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yqjB and yqjC are separated by 146 nucleotides yqjC and yqjD are separated by 37 nucleotides yqjD and yqjE are separated by 2 nucleotides yqjE and yqjK overlap by 11 nucleotides yqjK and yqjF are separated by 185 nucleotides yqjF and yqjG are separated by 69 nucleotides
b3096: yqjB - hypothetical protein (NCBI), at 3,246,461 to 3,246,844
yqjB
b3097: yqjC - orf, hypothetical protein (VIMSS), at 3,246,991 to 3,247,359
yqjC
b3098: yqjD - hypothetical protein (NCBI), at 3,247,397 to 3,247,702
yqjD
b3099: yqjE - conserved inner membrane protein (NCBI), at 3,247,705 to 3,248,109
yqjE
b3100: yqjK - hypothetical protein (NCBI), at 3,248,099 to 3,248,398
yqjK
b3101: yqjF - orf, hypothetical protein (VIMSS), at 3,248,584 to 3,248,976
yqjF
b3102: yqjG - predicted S-transferase (NCBI), at 3,249,046 to 3,250,032
yqjG
Position (kb)
3247
3248
3249 Strain fitness (log2 ratio)
-1
0
1 at 3246.751 kb on + strand, within yqjB at 3246.760 kb on + strand, within yqjB at 3246.760 kb on + strand, within yqjB at 3246.766 kb on - strand, within yqjB at 3246.781 kb on + strand, within yqjB at 3246.781 kb on + strand, within yqjB at 3246.789 kb on - strand, within yqjB at 3246.799 kb on + strand, within yqjB at 3246.834 kb on + strand at 3246.834 kb on + strand at 3246.873 kb on - strand at 3246.873 kb on - strand at 3246.937 kb on + strand at 3246.961 kb on - strand at 3246.961 kb on - strand at 3247.049 kb on + strand, within yqjC at 3247.054 kb on + strand, within yqjC at 3247.057 kb on - strand, within yqjC at 3247.057 kb on - strand, within yqjC at 3247.063 kb on + strand, within yqjC at 3247.067 kb on - strand, within yqjC at 3247.140 kb on + strand, within yqjC at 3247.140 kb on + strand, within yqjC at 3247.170 kb on + strand, within yqjC at 3247.177 kb on - strand, within yqjC at 3247.257 kb on - strand, within yqjC at 3247.257 kb on - strand, within yqjC at 3247.377 kb on - strand at 3247.382 kb on + strand at 3247.558 kb on + strand, within yqjD at 3247.616 kb on + strand, within yqjD at 3247.616 kb on + strand, within yqjD at 3247.649 kb on + strand, within yqjD at 3247.784 kb on + strand, within yqjE at 3247.801 kb on - strand, within yqjE at 3247.801 kb on - strand, within yqjE at 3247.995 kb on + strand, within yqjE at 3248.030 kb on + strand, within yqjE at 3248.030 kb on + strand, within yqjE at 3248.030 kb on + strand, within yqjE at 3248.041 kb on - strand, within yqjE at 3248.076 kb on + strand at 3248.140 kb on - strand, within yqjK at 3248.140 kb on - strand, within yqjK at 3248.162 kb on + strand, within yqjK at 3248.281 kb on + strand, within yqjK at 3248.281 kb on + strand, within yqjK at 3248.309 kb on - strand, within yqjK at 3248.309 kb on - strand, within yqjK at 3248.323 kb on - strand, within yqjK at 3248.326 kb on + strand, within yqjK at 3248.384 kb on - strand at 3248.676 kb on + strand, within yqjF at 3248.790 kb on + strand, within yqjF at 3248.797 kb on + strand, within yqjF at 3248.940 kb on + strand at 3248.948 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time48hr remove 3,246,751 + yqjB b3096 0.76 +0.3 3,246,760 + yqjB b3096 0.78 -0.1 3,246,760 + yqjB b3096 0.78 -0.6 3,246,766 - yqjB b3096 0.79 +0.4 3,246,781 + yqjB b3096 0.83 +0.1 3,246,781 + yqjB b3096 0.83 +0.6 3,246,789 - yqjB b3096 0.85 +0.1 3,246,799 + yqjB b3096 0.88 +0.4 3,246,834 + +0.6 3,246,834 + -0.3 3,246,873 - +0.7 3,246,873 - -0.2 3,246,937 + +0.2 3,246,961 - +0.1 3,246,961 - -0.1 3,247,049 + yqjC b3097 0.16 +0.3 3,247,054 + yqjC b3097 0.17 +0.2 3,247,057 - yqjC b3097 0.18 +0.2 3,247,057 - yqjC b3097 0.18 +0.7 3,247,063 + yqjC b3097 0.20 -0.4 3,247,067 - yqjC b3097 0.21 -0.6 3,247,140 + yqjC b3097 0.40 +0.5 3,247,140 + yqjC b3097 0.40 +0.1 3,247,170 + yqjC b3097 0.49 +0.0 3,247,177 - yqjC b3097 0.50 -0.0 3,247,257 - yqjC b3097 0.72 -0.1 3,247,257 - yqjC b3097 0.72 -0.2 3,247,377 - +0.3 3,247,382 + +0.8 3,247,558 + yqjD b3098 0.53 +0.2 3,247,616 + yqjD b3098 0.72 -0.2 3,247,616 + yqjD b3098 0.72 +0.2 3,247,649 + yqjD b3098 0.82 +0.4 3,247,784 + yqjE b3099 0.20 -0.2 3,247,801 - yqjE b3099 0.24 +0.2 3,247,801 - yqjE b3099 0.24 +0.9 3,247,995 + yqjE b3099 0.72 +0.3 3,248,030 + yqjE b3099 0.80 -0.0 3,248,030 + yqjE b3099 0.80 +0.3 3,248,030 + yqjE b3099 0.80 +0.8 3,248,041 - yqjE b3099 0.83 +0.9 3,248,076 + +0.2 3,248,140 - yqjK b3100 0.14 +0.8 3,248,140 - yqjK b3100 0.14 +0.4 3,248,162 + yqjK b3100 0.21 +0.4 3,248,281 + yqjK b3100 0.61 +0.3 3,248,281 + yqjK b3100 0.61 +0.7 3,248,309 - yqjK b3100 0.70 +0.6 3,248,309 - yqjK b3100 0.70 +0.6 3,248,323 - yqjK b3100 0.75 +0.3 3,248,326 + yqjK b3100 0.76 -0.1 3,248,384 - -0.3 3,248,676 + yqjF b3101 0.23 +0.0 3,248,790 + yqjF b3101 0.52 -0.0 3,248,797 + yqjF b3101 0.54 -0.1 3,248,940 + +0.2 3,248,948 - -0.1
Or see this region's nucleotide sequence