Experiment: LB + ciprofloxacin Time48hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfjX and yfjY are separated by 8 nucleotides yfjY and ypjJ are separated by 8 nucleotides ypjJ and yfjZ are separated by 37 nucleotides yfjZ and ypjF are separated by 20 nucleotides ypjF and psaA are separated by 189 nucleotides psaA and ypjA are separated by 160 nucleotides
b2643: yfjX - CP4-57 prophage; predicted antirestriction protein (NCBI), at 2,773,941 to 2,774,399
yfjX
b2644: yfjY - CP4-57 prophage; predicted DNA repair protein (NCBI), at 2,774,408 to 2,774,890
yfjY
b4548: ypjJ - hypothetical protein (NCBI), at 2,774,899 to 2,775,099
ypjJ
b2645: yfjZ - CP4-57 prophage; antitoxin of the YpjF-YfjZ toxin-antitoxin system (NCBI), at 2,775,137 to 2,775,454
yfjZ
b2646: ypjF - CP4-57 prophage; toxin of the YpjF-YfjZ toxin-antitoxin system (NCBI), at 2,775,475 to 2,775,804
ypjF
b4645: psaA - no description, at 2,775,994 to 2,776,007
psaA
b2647: ypjA - putative ATP-binding component of a transport system (VIMSS), at 2,776,168 to 2,780,748
ypjA
Position (kb)
2775
2776 Strain fitness (log2 ratio)
-1
0
1 at 2774.225 kb on + strand, within yfjX at 2774.260 kb on + strand, within yfjX at 2774.275 kb on + strand, within yfjX at 2774.338 kb on - strand, within yfjX at 2774.363 kb on + strand at 2774.400 kb on + strand at 2774.405 kb on - strand at 2774.419 kb on - strand at 2774.425 kb on - strand at 2774.493 kb on + strand, within yfjY at 2774.600 kb on + strand, within yfjY at 2774.600 kb on + strand, within yfjY at 2774.651 kb on + strand, within yfjY at 2774.654 kb on + strand, within yfjY at 2774.724 kb on - strand, within yfjY at 2774.726 kb on - strand, within yfjY at 2774.733 kb on + strand, within yfjY at 2774.783 kb on + strand, within yfjY at 2774.796 kb on - strand, within yfjY at 2774.967 kb on + strand, within ypjJ at 2775.033 kb on - strand, within ypjJ at 2775.050 kb on - strand, within ypjJ at 2775.066 kb on - strand, within ypjJ at 2775.116 kb on - strand at 2775.157 kb on - strand at 2775.157 kb on - strand at 2775.186 kb on + strand, within yfjZ at 2775.186 kb on + strand, within yfjZ at 2775.189 kb on - strand, within yfjZ at 2775.199 kb on - strand, within yfjZ at 2775.220 kb on - strand, within yfjZ at 2775.220 kb on - strand, within yfjZ at 2775.252 kb on + strand, within yfjZ at 2775.270 kb on - strand, within yfjZ at 2775.338 kb on - strand, within yfjZ at 2775.360 kb on + strand, within yfjZ at 2775.360 kb on + strand, within yfjZ at 2775.365 kb on + strand, within yfjZ at 2775.366 kb on - strand, within yfjZ at 2775.366 kb on - strand, within yfjZ at 2775.442 kb on - strand at 2775.488 kb on - strand at 2775.583 kb on + strand, within ypjF at 2775.646 kb on + strand, within ypjF at 2775.808 kb on + strand at 2775.833 kb on - strand at 2776.013 kb on - strand at 2776.146 kb on - strand at 2776.375 kb on + strand at 2776.406 kb on - strand at 2776.438 kb on - strand at 2776.438 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time48hr remove 2,774,225 + yfjX b2643 0.62 +0.2 2,774,260 + yfjX b2643 0.69 -0.1 2,774,275 + yfjX b2643 0.73 +0.1 2,774,338 - yfjX b2643 0.86 -0.3 2,774,363 + -0.2 2,774,400 + +0.5 2,774,405 - +0.2 2,774,419 - +0.1 2,774,425 - +0.1 2,774,493 + yfjY b2644 0.18 +0.1 2,774,600 + yfjY b2644 0.40 +0.4 2,774,600 + yfjY b2644 0.40 +0.1 2,774,651 + yfjY b2644 0.50 +0.3 2,774,654 + yfjY b2644 0.51 +1.3 2,774,724 - yfjY b2644 0.65 +0.2 2,774,726 - yfjY b2644 0.66 +0.3 2,774,733 + yfjY b2644 0.67 -0.7 2,774,783 + yfjY b2644 0.78 +0.3 2,774,796 - yfjY b2644 0.80 -0.4 2,774,967 + ypjJ b4548 0.34 +0.1 2,775,033 - ypjJ b4548 0.67 +0.3 2,775,050 - ypjJ b4548 0.75 +0.3 2,775,066 - ypjJ b4548 0.83 -0.1 2,775,116 - +0.6 2,775,157 - -0.5 2,775,157 - +0.5 2,775,186 + yfjZ b2645 0.15 +1.1 2,775,186 + yfjZ b2645 0.15 +0.4 2,775,189 - yfjZ b2645 0.16 -0.1 2,775,199 - yfjZ b2645 0.19 +0.1 2,775,220 - yfjZ b2645 0.26 +0.5 2,775,220 - yfjZ b2645 0.26 +0.5 2,775,252 + yfjZ b2645 0.36 +1.6 2,775,270 - yfjZ b2645 0.42 +0.2 2,775,338 - yfjZ b2645 0.63 +0.9 2,775,360 + yfjZ b2645 0.70 +0.0 2,775,360 + yfjZ b2645 0.70 +0.4 2,775,365 + yfjZ b2645 0.72 +0.1 2,775,366 - yfjZ b2645 0.72 +1.1 2,775,366 - yfjZ b2645 0.72 -0.9 2,775,442 - +0.3 2,775,488 - +0.3 2,775,583 + ypjF b2646 0.33 -0.2 2,775,646 + ypjF b2646 0.52 +0.0 2,775,808 + +0.7 2,775,833 - +0.3 2,776,013 - +0.8 2,776,146 - -0.1 2,776,375 + +0.1 2,776,406 - +0.2 2,776,438 - -0.6 2,776,438 - +0.2
Or see this region's nucleotide sequence