Strain Fitness in Escherichia coli BW25113 around b2565

Experiment: LB + ciprofloxacin Time48hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntacpS and pdxJ overlap by 1 nucleotidespdxJ and recO are separated by 11 nucleotidesrecO and era are separated by 11 nucleotidesera and rnc overlap by 4 nucleotides b2563: acpS - 4'-phosphopantetheinyl transferase (NCBI), at 2,698,640 to 2,699,020 acpS b2564: pdxJ - pyridoxal phosphate biosynthetic protein (NCBI), at 2,699,020 to 2,699,751 pdxJ b2565: recO - DNA repair protein RecO (NCBI), at 2,699,763 to 2,700,491 recO b2566: era - GTP-binding protein Era (NCBI), at 2,700,503 to 2,701,408 era b2567: rnc - ribonuclease III (NCBI), at 2,701,405 to 2,702,085 rnc Position (kb) 2699 2700 2701Strain fitness (log2 ratio) -1 0 1at 2699.085 kb on - strandat 2699.173 kb on - strand, within pdxJat 2699.178 kb on - strand, within pdxJat 2699.290 kb on - strand, within pdxJat 2699.465 kb on + strand, within pdxJat 2699.465 kb on + strand, within pdxJat 2699.504 kb on - strand, within pdxJat 2699.519 kb on + strand, within pdxJat 2699.536 kb on + strand, within pdxJat 2699.536 kb on + strand, within pdxJat 2699.713 kb on + strandat 2699.774 kb on - strandat 2699.774 kb on - strandat 2699.823 kb on + strandat 2699.924 kb on + strand, within recOat 2700.066 kb on - strand, within recOat 2700.126 kb on + strand, within recOat 2700.128 kb on + strand, within recOat 2700.219 kb on + strand, within recOat 2700.280 kb on - strand, within recOat 2700.280 kb on - strand, within recOat 2700.326 kb on - strand, within recOat 2700.359 kb on - strand, within recOat 2700.467 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time48hr
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2,699,085 - +0.3
2,699,173 - pdxJ b2564 0.21 +0.1
2,699,178 - pdxJ b2564 0.22 -0.7
2,699,290 - pdxJ b2564 0.37 +0.5
2,699,465 + pdxJ b2564 0.61 -0.5
2,699,465 + pdxJ b2564 0.61 -0.7
2,699,504 - pdxJ b2564 0.66 +0.1
2,699,519 + pdxJ b2564 0.68 +0.4
2,699,536 + pdxJ b2564 0.70 +0.6
2,699,536 + pdxJ b2564 0.70 -1.3
2,699,713 + -1.1
2,699,774 - -0.1
2,699,774 - +0.8
2,699,823 + +0.2
2,699,924 + recO b2565 0.22 +0.1
2,700,066 - recO b2565 0.42 +0.2
2,700,126 + recO b2565 0.50 -0.1
2,700,128 + recO b2565 0.50 +0.3
2,700,219 + recO b2565 0.63 +0.4
2,700,280 - recO b2565 0.71 -0.2
2,700,280 - recO b2565 0.71 +1.6
2,700,326 - recO b2565 0.77 +0.4
2,700,359 - recO b2565 0.82 +0.9
2,700,467 - -0.3

Or see this region's nucleotide sequence