Experiment: LB + ciprofloxacin Time48hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yecC and yecS overlap by 4 nucleotides yecS and yedO are separated by 14 nucleotides yedO and fliY are separated by 104 nucleotides fliY and fliZ are separated by 87 nucleotides
b1917: yecC - predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI), at 1,995,086 to 1,995,838
yecC
b1918: yecS - predicted transporter subunit: membrane component of ABC superfamily (NCBI), at 1,995,835 to 1,996,503
yecS
b1919: yedO - putative 1-aminocyclopropane-1-carboxylate deaminase (VIMSS), at 1,996,518 to 1,997,504
yedO
b1920: fliY - cystine transporter subunit (NCBI), at 1,997,609 to 1,998,409
fliY
b1921: fliZ - orf, hypothetical protein (VIMSS), at 1,998,497 to 1,999,048
fliZ
Position (kb)
1996
1997
1998 Strain fitness (log2 ratio)
-1
0
1 at 1995.592 kb on + strand, within yecC at 1995.598 kb on - strand, within yecC at 1995.598 kb on - strand, within yecC at 1995.688 kb on + strand, within yecC at 1995.771 kb on - strand at 1995.777 kb on - strand at 1995.777 kb on - strand at 1995.779 kb on + strand at 1995.779 kb on + strand at 1995.779 kb on + strand at 1995.779 kb on + strand at 1995.880 kb on + strand at 1995.880 kb on + strand at 1995.968 kb on + strand, within yecS at 1995.976 kb on - strand, within yecS at 1996.355 kb on + strand, within yecS at 1996.445 kb on - strand at 1996.445 kb on - strand at 1996.446 kb on - strand at 1996.778 kb on - strand, within yedO at 1997.064 kb on - strand, within yedO at 1997.231 kb on - strand, within yedO at 1997.231 kb on - strand, within yedO at 1997.256 kb on - strand, within yedO at 1997.658 kb on + strand at 1997.811 kb on + strand, within fliY at 1997.811 kb on + strand, within fliY at 1997.931 kb on - strand, within fliY at 1998.266 kb on + strand, within fliY at 1998.375 kb on + strand at 1998.375 kb on + strand at 1998.387 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time48hr remove 1,995,592 + yecC b1917 0.67 +0.6 1,995,598 - yecC b1917 0.68 -0.2 1,995,598 - yecC b1917 0.68 +0.1 1,995,688 + yecC b1917 0.80 +0.6 1,995,771 - +0.2 1,995,777 - -0.3 1,995,777 - -0.5 1,995,779 + -0.1 1,995,779 + +0.1 1,995,779 + +0.4 1,995,779 + -0.0 1,995,880 + +0.1 1,995,880 + +1.2 1,995,968 + yecS b1918 0.20 +0.3 1,995,976 - yecS b1918 0.21 -0.1 1,996,355 + yecS b1918 0.78 -0.5 1,996,445 - -0.0 1,996,445 - +1.0 1,996,446 - +0.2 1,996,778 - yedO b1919 0.26 +0.8 1,997,064 - yedO b1919 0.55 +0.4 1,997,231 - yedO b1919 0.72 +1.3 1,997,231 - yedO b1919 0.72 -0.2 1,997,256 - yedO b1919 0.75 -0.2 1,997,658 + +0.4 1,997,811 + fliY b1920 0.25 +0.1 1,997,811 + fliY b1920 0.25 -0.7 1,997,931 - fliY b1920 0.40 +0.3 1,998,266 + fliY b1920 0.82 -0.6 1,998,375 + -0.8 1,998,375 + -0.2 1,998,387 - -0.1
Or see this region's nucleotide sequence