Strain Fitness in Escherichia coli BW25113 around b1875

Experiment: LB + ciprofloxacin Time48hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcutC and yecM are separated by 13 nucleotidesyecM and argS are separated by 215 nucleotides b1874: cutC - copper homeostasis protein (NCBI), at 1,956,544 to 1,957,290 cutC b1875: yecM - orf, hypothetical protein (VIMSS), at 1,957,304 to 1,957,870 yecM b1876: argS - arginyl-tRNA synthetase (NCBI), at 1,958,086 to 1,959,819 argS Position (kb) 1957 1958Strain fitness (log2 ratio) -2 -1 0 1at 1956.405 kb on - strandat 1956.407 kb on - strandat 1956.407 kb on - strandat 1956.416 kb on - strandat 1956.416 kb on - strandat 1956.417 kb on - strandat 1956.417 kb on - strandat 1956.417 kb on - strandat 1956.417 kb on - strandat 1956.417 kb on - strandat 1956.417 kb on - strandat 1956.417 kb on - strandat 1956.505 kb on - strandat 1956.561 kb on + strandat 1956.628 kb on + strand, within cutCat 1956.628 kb on + strand, within cutCat 1956.709 kb on + strand, within cutCat 1956.764 kb on - strand, within cutCat 1957.248 kb on + strandat 1957.416 kb on + strand, within yecMat 1957.473 kb on - strand, within yecMat 1957.588 kb on - strand, within yecMat 1957.677 kb on + strand, within yecMat 1957.760 kb on - strand, within yecMat 1957.810 kb on - strand, within yecMat 1957.810 kb on - strand, within yecMat 1958.016 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time48hr
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1,956,405 - +0.3
1,956,407 - +1.1
1,956,407 - +0.1
1,956,416 - +0.4
1,956,416 - -0.1
1,956,417 - +0.6
1,956,417 - -0.2
1,956,417 - -0.9
1,956,417 - -0.1
1,956,417 - +0.6
1,956,417 - -0.5
1,956,417 - +0.9
1,956,505 - -0.4
1,956,561 + +0.3
1,956,628 + cutC b1874 0.11 -0.3
1,956,628 + cutC b1874 0.11 -2.5
1,956,709 + cutC b1874 0.22 -0.6
1,956,764 - cutC b1874 0.29 +0.1
1,957,248 + -0.0
1,957,416 + yecM b1875 0.20 +0.0
1,957,473 - yecM b1875 0.30 -0.3
1,957,588 - yecM b1875 0.50 +0.7
1,957,677 + yecM b1875 0.66 +0.9
1,957,760 - yecM b1875 0.80 +0.7
1,957,810 - yecM b1875 0.89 +0.8
1,957,810 - yecM b1875 0.89 -0.0
1,958,016 + +0.2

Or see this region's nucleotide sequence