Strain Fitness in Escherichia coli BW25113 around b1603

Experiment: LB + ciprofloxacin Time48hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpntB and pntA are separated by 10 nucleotidespntA and ydgH are separated by 523 nucleotides b1602: pntB - pyridine nucleotide transhydrogenase (NCBI), at 1,672,996 to 1,674,384 pntB b1603: pntA - NAD(P) transhydrogenase subunit alpha (NCBI), at 1,674,395 to 1,675,927 pntA b1604: ydgH - hypothetical protein (NCBI), at 1,676,451 to 1,677,395 ydgH Position (kb) 1674 1675 1676Strain fitness (log2 ratio) -1 0 1at 1673.501 kb on - strand, within pntBat 1673.579 kb on - strand, within pntBat 1673.579 kb on - strand, within pntBat 1673.667 kb on + strand, within pntBat 1673.672 kb on + strand, within pntBat 1673.672 kb on + strand, within pntBat 1673.878 kb on + strand, within pntBat 1673.878 kb on + strand, within pntBat 1674.216 kb on + strand, within pntBat 1674.370 kb on - strandat 1674.385 kb on + strandat 1674.511 kb on - strandat 1674.521 kb on - strandat 1674.521 kb on - strandat 1674.523 kb on + strandat 1674.583 kb on - strand, within pntAat 1674.865 kb on + strand, within pntAat 1674.894 kb on - strand, within pntAat 1675.064 kb on + strand, within pntAat 1675.064 kb on + strand, within pntAat 1675.987 kb on + strandat 1675.987 kb on + strandat 1675.997 kb on - strandat 1675.997 kb on - strandat 1676.126 kb on - strandat 1676.139 kb on - strandat 1676.139 kb on - strandat 1676.139 kb on - strandat 1676.139 kb on - strandat 1676.154 kb on - strandat 1676.257 kb on - strandat 1676.257 kb on - strandat 1676.418 kb on + strandat 1676.523 kb on + strandat 1676.770 kb on + strand, within ydgHat 1676.818 kb on - strand, within ydgH

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time48hr
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1,673,501 - pntB b1602 0.36 -0.0
1,673,579 - pntB b1602 0.42 -0.0
1,673,579 - pntB b1602 0.42 +0.1
1,673,667 + pntB b1602 0.48 +0.1
1,673,672 + pntB b1602 0.49 +0.4
1,673,672 + pntB b1602 0.49 -0.2
1,673,878 + pntB b1602 0.63 +0.3
1,673,878 + pntB b1602 0.63 +0.0
1,674,216 + pntB b1602 0.88 +1.3
1,674,370 - +0.6
1,674,385 + +0.5
1,674,511 - +0.7
1,674,521 - +0.2
1,674,521 - +0.8
1,674,523 + +0.3
1,674,583 - pntA b1603 0.12 +0.5
1,674,865 + pntA b1603 0.31 +0.3
1,674,894 - pntA b1603 0.33 +0.8
1,675,064 + pntA b1603 0.44 +0.2
1,675,064 + pntA b1603 0.44 +0.2
1,675,987 + +0.5
1,675,987 + +0.4
1,675,997 - +0.8
1,675,997 - +0.2
1,676,126 - -0.4
1,676,139 - +0.1
1,676,139 - +0.1
1,676,139 - +0.3
1,676,139 - +0.0
1,676,154 - +0.4
1,676,257 - +0.1
1,676,257 - +0.7
1,676,418 + +0.1
1,676,523 + +0.3
1,676,770 + ydgH b1604 0.34 +1.6
1,676,818 - ydgH b1604 0.39 +0.1

Or see this region's nucleotide sequence