Strain Fitness in Escherichia coli BW25113 around b1113

Experiment: LB + ciprofloxacin Time48hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntycfQ and ycfR are separated by 240 nucleotidesycfR and ycfS are separated by 81 nucleotidesycfS and mfd are separated by 143 nucleotides b1111: ycfQ - orf, hypothetical protein (VIMSS), at 1,167,423 to 1,168,055 ycfQ b1112: ycfR - hypothetical protein (NCBI), at 1,168,296 to 1,168,553 ycfR b1113: ycfS - hypothetical protein (NCBI), at 1,168,635 to 1,169,597 ycfS b1114: mfd - transcription-repair coupling factor (NCBI), at 1,169,741 to 1,173,187 mfd Position (kb) 1168 1169 1170Strain fitness (log2 ratio) -1 0 1at 1167.640 kb on + strand, within ycfQat 1167.840 kb on + strand, within ycfQat 1167.840 kb on - strand, within ycfQat 1167.949 kb on - strand, within ycfQat 1168.045 kb on - strandat 1168.045 kb on - strandat 1168.075 kb on - strandat 1168.075 kb on - strandat 1168.746 kb on + strand, within ycfSat 1168.766 kb on + strand, within ycfSat 1168.802 kb on + strand, within ycfSat 1168.805 kb on + strand, within ycfSat 1168.870 kb on + strand, within ycfSat 1168.925 kb on - strand, within ycfSat 1169.013 kb on + strand, within ycfSat 1169.180 kb on + strand, within ycfSat 1169.204 kb on + strand, within ycfSat 1169.204 kb on + strand, within ycfSat 1169.259 kb on - strand, within ycfSat 1169.270 kb on + strand, within ycfSat 1169.654 kb on - strandat 1169.664 kb on + strandat 1169.664 kb on + strandat 1169.666 kb on + strandat 1169.674 kb on + strandat 1169.674 kb on + strandat 1169.682 kb on - strandat 1170.000 kb on + strandat 1170.000 kb on + strandat 1170.148 kb on - strand, within mfdat 1170.304 kb on - strand, within mfd

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time48hr
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1,167,640 + ycfQ b1111 0.34 -0.2
1,167,840 + ycfQ b1111 0.66 +0.3
1,167,840 - ycfQ b1111 0.66 +0.3
1,167,949 - ycfQ b1111 0.83 +0.4
1,168,045 - -0.2
1,168,045 - -0.3
1,168,075 - +0.7
1,168,075 - +0.4
1,168,746 + ycfS b1113 0.12 +0.2
1,168,766 + ycfS b1113 0.14 -0.2
1,168,802 + ycfS b1113 0.17 +0.5
1,168,805 + ycfS b1113 0.18 +0.8
1,168,870 + ycfS b1113 0.24 +0.5
1,168,925 - ycfS b1113 0.30 +0.6
1,169,013 + ycfS b1113 0.39 +0.5
1,169,180 + ycfS b1113 0.57 +0.1
1,169,204 + ycfS b1113 0.59 +0.2
1,169,204 + ycfS b1113 0.59 +0.6
1,169,259 - ycfS b1113 0.65 +0.5
1,169,270 + ycfS b1113 0.66 +0.6
1,169,654 - +0.3
1,169,664 + +0.2
1,169,664 + +0.3
1,169,666 + -0.1
1,169,674 + +0.2
1,169,674 + +0.2
1,169,682 - +0.1
1,170,000 + +0.6
1,170,000 + +0.7
1,170,148 - mfd b1114 0.12 -0.0
1,170,304 - mfd b1114 0.16 +0.1

Or see this region's nucleotide sequence