Experiment: LB + ciprofloxacin Time48hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ykgL and ykgO are separated by 34 nucleotides ykgO and ykgM overlap by 1 nucleotides ykgM and ykgR are separated by 363 nucleotides ykgR and ykgP are separated by 473 nucleotides
b0295: ykgL - hypothetical protein (NCBI), at 311,336 to 311,563
ykgL
b4506: ykgO - rpmJ (L36) paralog (NCBI), at 311,598 to 311,738
ykgO
b0296: ykgM - 50S ribosomal protein L31 (NCBI), at 311,738 to 312,001
ykgM
b4671: ykgR - expressed protein (RefSeq), at 312,365 to 312,466
ykgR
b4630: ykgP - no description, at 312,940 to 313,029
ykgP
Position (kb) 311
312
313 Strain fitness (log2 ratio) -2
-1
0
1
2 at 310.829 kb on + strand at 310.880 kb on - strand at 310.891 kb on - strand at 310.891 kb on - strand at 310.892 kb on - strand at 310.892 kb on - strand at 310.894 kb on - strand at 310.904 kb on - strand at 311.087 kb on - strand at 311.091 kb on - strand at 311.100 kb on + strand at 311.100 kb on + strand at 311.114 kb on - strand at 311.138 kb on - strand at 311.172 kb on - strand at 311.172 kb on - strand at 311.194 kb on - strand at 311.222 kb on - strand at 311.222 kb on - strand at 311.256 kb on + strand at 311.260 kb on + strand at 311.260 kb on + strand at 311.352 kb on + strand at 311.360 kb on + strand, within ykgL at 311.360 kb on + strand, within ykgL at 311.364 kb on - strand, within ykgL at 311.370 kb on + strand, within ykgL at 311.380 kb on + strand, within ykgL at 311.385 kb on + strand, within ykgL at 311.385 kb on + strand, within ykgL at 311.396 kb on + strand, within ykgL at 311.396 kb on + strand, within ykgL at 311.402 kb on + strand, within ykgL at 311.402 kb on + strand, within ykgL at 311.418 kb on - strand, within ykgL at 311.470 kb on + strand, within ykgL at 311.470 kb on + strand, within ykgL at 311.525 kb on + strand, within ykgL at 311.626 kb on + strand, within ykgO at 311.626 kb on + strand, within ykgO at 311.634 kb on - strand, within ykgO at 311.634 kb on - strand, within ykgO at 311.634 kb on - strand, within ykgO at 311.722 kb on + strand, within ykgO at 311.722 kb on + strand, within ykgO at 311.829 kb on + strand, within ykgM at 311.829 kb on - strand, within ykgM at 311.944 kb on - strand, within ykgM at 311.977 kb on + strand at 311.985 kb on - strand at 312.124 kb on + strand at 312.124 kb on + strand at 312.125 kb on + strand at 312.144 kb on + strand at 312.146 kb on + strand at 312.146 kb on + strand at 312.215 kb on + strand at 312.216 kb on + strand at 312.258 kb on + strand at 312.258 kb on + strand at 312.260 kb on + strand at 312.269 kb on + strand at 312.488 kb on - strand at 312.488 kb on - strand at 312.488 kb on - strand at 312.488 kb on - strand at 312.490 kb on - strand at 312.505 kb on - strand at 312.520 kb on - strand at 312.522 kb on - strand at 312.570 kb on + strand at 312.586 kb on + strand at 312.596 kb on + strand at 312.605 kb on + strand at 312.615 kb on - strand at 312.619 kb on + strand at 312.642 kb on - strand at 312.648 kb on + strand at 312.648 kb on + strand at 312.650 kb on + strand at 312.658 kb on - strand at 312.660 kb on + strand at 312.660 kb on + strand at 312.664 kb on + strand at 312.666 kb on + strand at 312.672 kb on - strand at 312.674 kb on - strand at 312.677 kb on + strand at 312.679 kb on - strand at 312.679 kb on - strand at 312.690 kb on - strand at 312.690 kb on - strand at 312.693 kb on - strand at 312.695 kb on + strand at 312.712 kb on - strand at 312.721 kb on + strand at 312.721 kb on + strand at 312.721 kb on + strand at 312.729 kb on + strand at 312.731 kb on - strand at 312.743 kb on + strand at 312.766 kb on + strand at 312.784 kb on + strand at 312.785 kb on - strand at 312.800 kb on + strand at 312.800 kb on + strand at 312.800 kb on + strand at 312.802 kb on + strand at 312.805 kb on + strand at 312.810 kb on + strand at 312.816 kb on + strand at 312.818 kb on + strand at 312.822 kb on + strand at 312.822 kb on + strand at 312.829 kb on + strand at 312.831 kb on + strand at 312.831 kb on + strand at 312.837 kb on - strand at 312.837 kb on - strand at 312.839 kb on - strand at 312.841 kb on - strand at 312.841 kb on - strand at 312.841 kb on - strand at 312.854 kb on - strand at 312.854 kb on - strand at 312.968 kb on - strand, within ykgP at 312.973 kb on - strand, within ykgP at 312.973 kb on - strand, within ykgP
Per-strain Table
Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time48hr remove 310,829 + +0.4 310,880 - +0.4 310,891 - +0.4 310,891 - +0.4 310,892 - +0.4 310,892 - +2.4 310,894 - -0.6 310,904 - +0.1 311,087 - -0.8 311,091 - +0.6 311,100 + -0.2 311,100 + -0.0 311,114 - -0.6 311,138 - +1.1 311,172 - +0.9 311,172 - +0.6 311,194 - -0.3 311,222 - +1.1 311,222 - -0.2 311,256 + +0.2 311,260 + +0.2 311,260 + +0.0 311,352 + +0.6 311,360 + ykgL b0295 0.11 -0.6 311,360 + ykgL b0295 0.11 +0.6 311,364 - ykgL b0295 0.12 +0.4 311,370 + ykgL b0295 0.15 +0.3 311,380 + ykgL b0295 0.19 +0.7 311,385 + ykgL b0295 0.21 +0.3 311,385 + ykgL b0295 0.21 +0.6 311,396 + ykgL b0295 0.26 +1.1 311,396 + ykgL b0295 0.26 -0.0 311,402 + ykgL b0295 0.29 +0.4 311,402 + ykgL b0295 0.29 -0.1 311,418 - ykgL b0295 0.36 +2.0 311,470 + ykgL b0295 0.59 -0.6 311,470 + ykgL b0295 0.59 +0.1 311,525 + ykgL b0295 0.83 +0.4 311,626 + ykgO b4506 0.20 -1.2 311,626 + ykgO b4506 0.20 +2.7 311,634 - ykgO b4506 0.26 -0.5 311,634 - ykgO b4506 0.26 +0.4 311,634 - ykgO b4506 0.26 +0.6 311,722 + ykgO b4506 0.88 +2.0 311,722 + ykgO b4506 0.88 +0.4 311,829 + ykgM b0296 0.34 +0.5 311,829 - ykgM b0296 0.34 +0.2 311,944 - ykgM b0296 0.78 +0.4 311,977 + +0.6 311,985 - +0.0 312,124 + +0.1 312,124 + -0.2 312,125 + -0.2 312,144 + +0.2 312,146 + -0.4 312,146 + -0.3 312,215 + +0.2 312,216 + -0.2 312,258 + +0.1 312,258 + +0.1 312,260 + -0.1 312,269 + +0.1 312,488 - +0.4 312,488 - +0.2 312,488 - +0.1 312,488 - +0.7 312,490 - +0.6 312,505 - -0.2 312,520 - +0.0 312,522 - +0.7 312,570 + -0.0 312,586 + +0.4 312,596 + +0.4 312,605 + -0.7 312,615 - +0.7 312,619 + -1.2 312,642 - +0.1 312,648 + +1.4 312,648 + +0.8 312,650 + -1.6 312,658 - +2.0 312,660 + -1.0 312,660 + +0.5 312,664 + -0.1 312,666 + +0.4 312,672 - -0.4 312,674 - +2.1 312,677 + +0.4 312,679 - +0.8 312,679 - +1.2 312,690 - +2.7 312,690 - +1.4 312,693 - +0.4 312,695 + +0.0 312,712 - +0.1 312,721 + -0.3 312,721 + +1.2 312,721 + -0.1 312,729 + +0.4 312,731 - +2.4 312,743 + +2.0 312,766 + +0.4 312,784 + -2.2 312,785 - +0.4 312,800 + +0.4 312,800 + -1.2 312,800 + -2.2 312,802 + +0.2 312,805 + +1.4 312,810 + -0.6 312,816 + +0.4 312,818 + +0.4 312,822 + +1.4 312,822 + +0.4 312,829 + +0.4 312,831 + +0.4 312,831 + +0.4 312,837 - +0.4 312,837 - +0.7 312,839 - +0.4 312,841 - +0.4 312,841 - +1.4 312,841 - -0.3 312,854 - +1.4 312,854 - -0.0 312,968 - ykgP b4630 0.31 +0.8 312,973 - ykgP b4630 0.37 -0.1 312,973 - ykgP b4630 0.37 +0.1
Or see this region's nucleotide sequence