Strain Fitness in Escherichia coli BW25113 around b3404
Experiment: LB + carbenicillin Time24hr
Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.
Per-strain Table
Position | Strand | Gene | LocusTag | Fraction | LB + carbenicillin Time24hr |
---|---|---|---|---|---|
remove | |||||
3,531,647 | + | pck | b3403 | 0.50 | +0.2 |
3,531,662 | - | pck | b3403 | 0.51 | +0.3 |
3,531,662 | - | pck | b3403 | 0.51 | +0.6 |
3,531,747 | - | pck | b3403 | 0.56 | -0.1 |
3,531,799 | - | pck | b3403 | 0.59 | +0.2 |
3,531,840 | + | pck | b3403 | 0.62 | +0.2 |
3,531,848 | - | pck | b3403 | 0.62 | +0.1 |
3,531,868 | + | pck | b3403 | 0.63 | -0.4 |
3,531,985 | + | pck | b3403 | 0.71 | -0.1 |
3,532,017 | + | pck | b3403 | 0.73 | +0.0 |
3,532,027 | + | pck | b3403 | 0.73 | +0.1 |
3,532,050 | + | pck | b3403 | 0.75 | +0.0 |
3,532,092 | + | pck | b3403 | 0.77 | -0.0 |
3,532,092 | + | pck | b3403 | 0.77 | +0.2 |
3,532,099 | + | pck | b3403 | 0.78 | +0.2 |
3,532,122 | - | pck | b3403 | 0.79 | +0.0 |
3,532,137 | - | pck | b3403 | 0.80 | +0.1 |
3,532,144 | + | pck | b3403 | 0.80 | +0.7 |
3,532,148 | - | pck | b3403 | 0.81 | +0.0 |
3,532,153 | + | pck | b3403 | 0.81 | +0.2 |
3,532,171 | - | pck | b3403 | 0.82 | +0.0 |
3,532,178 | + | pck | b3403 | 0.82 | -0.0 |
3,532,178 | + | pck | b3403 | 0.82 | +0.1 |
3,532,225 | + | pck | b3403 | 0.85 | +1.1 |
3,532,233 | - | pck | b3403 | 0.86 | +0.3 |
3,532,235 | + | pck | b3403 | 0.86 | +0.2 |
3,532,270 | - | pck | b3403 | 0.88 | +0.1 |
3,532,270 | - | pck | b3403 | 0.88 | +0.2 |
3,532,277 | - | pck | b3403 | 0.89 | -0.1 |
3,532,277 | - | pck | b3403 | 0.89 | +0.8 |
3,532,325 | - | +0.3 | |||
3,532,325 | - | +0.2 | |||
3,532,327 | + | +0.4 | |||
3,532,329 | - | +0.3 | |||
3,532,335 | - | +0.4 | |||
3,532,335 | - | +0.1 | |||
3,532,335 | - | +0.5 | |||
3,532,411 | - | +0.1 | |||
3,532,434 | + | +0.1 | |||
3,532,434 | + | +0.7 | |||
3,532,446 | - | +0.1 | |||
3,532,559 | - | -0.0 | |||
3,532,565 | - | -0.1 | |||
3,532,630 | - | -0.5 | |||
3,532,646 | - | -1.4 | |||
3,532,646 | - | +1.0 | |||
3,532,671 | - | -1.6 | |||
3,532,671 | - | -1.4 | |||
3,532,676 | + | envZ | b3404 | 0.10 | +0.4 |
3,532,676 | + | envZ | b3404 | 0.10 | +0.1 |
3,532,676 | + | envZ | b3404 | 0.10 | -1.9 |
3,532,676 | + | envZ | b3404 | 0.10 | +0.4 |
3,532,689 | + | envZ | b3404 | 0.11 | +0.3 |
3,532,733 | - | envZ | b3404 | 0.14 | +0.8 |
3,532,735 | + | envZ | b3404 | 0.15 | +0.0 |
3,532,736 | + | envZ | b3404 | 0.15 | -0.7 |
3,532,737 | + | envZ | b3404 | 0.15 | -2.5 |
3,532,777 | - | envZ | b3404 | 0.18 | +0.3 |
3,532,804 | - | envZ | b3404 | 0.20 | -0.1 |
3,532,835 | + | envZ | b3404 | 0.22 | -1.1 |
3,532,848 | - | envZ | b3404 | 0.23 | -0.3 |
3,532,853 | + | envZ | b3404 | 0.23 | -0.3 |
3,532,870 | - | envZ | b3404 | 0.25 | -1.4 |
3,532,870 | - | envZ | b3404 | 0.25 | -1.6 |
3,532,888 | - | envZ | b3404 | 0.26 | -1.2 |
3,532,919 | + | envZ | b3404 | 0.28 | -0.6 |
3,532,970 | - | envZ | b3404 | 0.32 | +0.8 |
3,532,973 | + | envZ | b3404 | 0.32 | +0.2 |
3,533,031 | - | envZ | b3404 | 0.36 | -0.2 |
3,533,093 | + | envZ | b3404 | 0.41 | -0.7 |
3,533,139 | - | envZ | b3404 | 0.44 | -0.6 |
3,533,159 | + | envZ | b3404 | 0.46 | -0.1 |
3,533,203 | + | envZ | b3404 | 0.49 | -0.8 |
3,533,264 | + | envZ | b3404 | 0.54 | -0.5 |
3,533,306 | + | envZ | b3404 | 0.57 | +0.8 |
3,533,314 | - | envZ | b3404 | 0.57 | -0.4 |
3,533,326 | - | envZ | b3404 | 0.58 | -1.1 |
3,533,342 | + | envZ | b3404 | 0.59 | -0.6 |
3,533,350 | - | envZ | b3404 | 0.60 | -0.5 |
3,533,350 | - | envZ | b3404 | 0.60 | -0.3 |
3,533,360 | - | envZ | b3404 | 0.61 | -0.5 |
3,533,360 | - | envZ | b3404 | 0.61 | -0.6 |
3,533,398 | + | envZ | b3404 | 0.64 | +0.4 |
3,533,400 | + | envZ | b3404 | 0.64 | -0.3 |
3,533,406 | - | envZ | b3404 | 0.64 | -0.6 |
3,533,458 | + | envZ | b3404 | 0.68 | +1.9 |
3,533,460 | + | envZ | b3404 | 0.68 | +0.1 |
3,533,492 | + | envZ | b3404 | 0.71 | -2.0 |
3,533,519 | + | envZ | b3404 | 0.73 | -0.3 |
3,533,523 | - | envZ | b3404 | 0.73 | -0.3 |
3,533,571 | - | envZ | b3404 | 0.76 | -0.6 |
3,533,571 | - | envZ | b3404 | 0.76 | -0.9 |
3,533,572 | - | envZ | b3404 | 0.76 | -0.7 |
3,533,599 | - | envZ | b3404 | 0.78 | -0.7 |
3,533,629 | - | envZ | b3404 | 0.81 | -0.5 |
3,533,676 | + | envZ | b3404 | 0.84 | +0.4 |
3,533,693 | + | envZ | b3404 | 0.85 | +1.9 |
3,533,723 | + | envZ | b3404 | 0.88 | -0.8 |
3,533,832 | + | +0.1 | |||
3,533,853 | - | +0.7 | |||
3,533,853 | - | +1.2 | |||
3,533,871 | - | +0.0 | |||
3,533,892 | + | -0.5 | |||
3,533,900 | - | -1.1 | |||
3,533,900 | - | -0.3 | |||
3,533,900 | - | +0.2 | |||
3,533,900 | - | -0.1 | |||
3,533,907 | + | -1.3 | |||
3,533,926 | + | -1.2 | |||
3,533,933 | - | +2.5 | |||
3,533,950 | - | +0.4 | |||
3,534,010 | - | ompR | b3405 | 0.17 | -1.6 |
3,534,010 | - | ompR | b3405 | 0.17 | -1.1 |
3,534,057 | - | ompR | b3405 | 0.24 | -0.6 |
3,534,093 | + | ompR | b3405 | 0.29 | +0.7 |
3,534,097 | + | ompR | b3405 | 0.29 | -0.8 |
3,534,097 | + | ompR | b3405 | 0.29 | +1.9 |
3,534,097 | + | ompR | b3405 | 0.29 | -1.1 |
3,534,105 | - | ompR | b3405 | 0.30 | -1.4 |
3,534,105 | - | ompR | b3405 | 0.30 | -1.2 |
3,534,129 | - | ompR | b3405 | 0.34 | -1.8 |
3,534,168 | - | ompR | b3405 | 0.39 | -0.7 |
3,534,206 | + | ompR | b3405 | 0.44 | +0.7 |
3,534,228 | - | ompR | b3405 | 0.47 | +0.7 |
3,534,257 | - | ompR | b3405 | 0.51 | +1.4 |
3,534,260 | - | ompR | b3405 | 0.52 | -0.6 |
3,534,267 | - | ompR | b3405 | 0.53 | +1.5 |
3,534,267 | - | ompR | b3405 | 0.53 | +1.7 |
3,534,358 | - | ompR | b3405 | 0.65 | +2.8 |
3,534,436 | + | ompR | b3405 | 0.76 | +1.3 |
3,534,447 | + | ompR | b3405 | 0.78 | -0.6 |
3,534,456 | - | ompR | b3405 | 0.79 | -0.5 |
3,534,509 | + | ompR | b3405 | 0.86 | -1.4 |
3,534,514 | + | ompR | b3405 | 0.87 | -1.4 |
3,534,514 | + | ompR | b3405 | 0.87 | +0.5 |
3,534,522 | - | ompR | b3405 | 0.88 | -0.8 |
3,534,541 | - | +3.3 | |||
3,534,541 | - | -0.1 | |||
3,534,544 | + | +1.9 | |||
3,534,606 | - | -1.0 | |||
3,534,608 | + | -0.2 | |||
3,534,608 | + | -0.5 | |||
3,534,616 | + | +0.1 | |||
3,534,625 | - | +0.7 | |||
3,534,632 | + | +0.5 | |||
3,534,684 | - | -0.2 | |||
3,534,724 | - | -0.5 | |||
3,534,783 | - | +0.8 | |||
3,534,851 | - | -0.6 | |||
3,534,851 | - | -0.2 | |||
3,534,852 | + | +0.2 | |||
3,534,861 | - | -0.1 | |||
3,534,861 | - | +0.2 | |||
3,534,889 | + | greB | b3406 | 0.12 | -0.4 |
3,534,889 | + | greB | b3406 | 0.12 | +0.3 |
Or see this region's nucleotide sequence