Strain Fitness in Escherichia coli BW25113 around b3021

Experiment: LB + carbenicillin Time24hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntygiS and ygiT are separated by 132 nucleotidesygiT and b3022 are separated by 1 nucleotidesb3022 and ygiV are separated by 204 nucleotides b3020: ygiS - predicted transporter subunit: periplasmic-binding component of ABC superfamily (NCBI), at 3,164,133 to 3,165,740 ygiS b3021: ygiT - predicted DNA-binding transcriptional regulator (NCBI), at 3,165,873 to 3,166,268 ygiT b3022: b3022 - predicted cyanide hydratase (NCBI), at 3,166,270 to 3,166,566 b3022 b3023: ygiV - predicted transcriptional regulator (NCBI), at 3,166,771 to 3,167,253 ygiV Position (kb) 3165 3166 3167Strain fitness (log2 ratio) -1 0 1 2at 3164.942 kb on + strand, within ygiSat 3164.953 kb on - strand, within ygiSat 3164.953 kb on - strand, within ygiSat 3164.984 kb on - strand, within ygiSat 3164.984 kb on - strand, within ygiSat 3164.984 kb on - strand, within ygiSat 3165.101 kb on + strand, within ygiSat 3165.112 kb on + strand, within ygiSat 3165.112 kb on + strand, within ygiSat 3165.114 kb on + strand, within ygiSat 3165.122 kb on - strand, within ygiSat 3165.147 kb on - strand, within ygiSat 3165.147 kb on - strand, within ygiSat 3165.196 kb on - strand, within ygiSat 3165.256 kb on - strand, within ygiSat 3165.256 kb on - strand, within ygiSat 3165.304 kb on + strand, within ygiSat 3165.442 kb on + strand, within ygiSat 3165.443 kb on - strand, within ygiSat 3165.477 kb on + strand, within ygiSat 3165.516 kb on - strand, within ygiSat 3165.530 kb on - strand, within ygiSat 3165.530 kb on - strand, within ygiSat 3165.544 kb on + strand, within ygiSat 3165.545 kb on - strand, within ygiSat 3165.545 kb on - strand, within ygiSat 3165.552 kb on - strand, within ygiSat 3165.574 kb on + strand, within ygiSat 3165.583 kb on - strandat 3165.583 kb on - strandat 3165.616 kb on - strandat 3165.667 kb on - strandat 3165.682 kb on + strandat 3165.682 kb on + strandat 3165.687 kb on - strandat 3165.692 kb on + strandat 3165.692 kb on - strandat 3165.692 kb on - strandat 3165.700 kb on + strandat 3165.736 kb on + strandat 3165.745 kb on - strandat 3165.748 kb on - strandat 3165.770 kb on + strandat 3165.775 kb on + strandat 3165.784 kb on - strandat 3165.798 kb on + strandat 3165.798 kb on + strandat 3165.822 kb on + strandat 3165.822 kb on + strandat 3165.895 kb on + strandat 3165.903 kb on + strandat 3165.914 kb on - strand, within ygiTat 3165.946 kb on - strand, within ygiTat 3165.950 kb on - strand, within ygiTat 3165.950 kb on - strand, within ygiTat 3165.985 kb on - strand, within ygiTat 3166.018 kb on - strand, within ygiTat 3166.093 kb on + strand, within ygiTat 3166.285 kb on - strandat 3166.285 kb on - strandat 3166.286 kb on - strandat 3166.290 kb on - strandat 3166.290 kb on - strandat 3166.299 kb on - strandat 3166.308 kb on - strand, within b3022at 3166.308 kb on - strand, within b3022at 3166.325 kb on - strand, within b3022at 3166.330 kb on - strand, within b3022at 3166.330 kb on - strand, within b3022at 3166.335 kb on - strand, within b3022at 3166.337 kb on + strand, within b3022at 3166.345 kb on - strand, within b3022at 3166.345 kb on - strand, within b3022at 3166.346 kb on - strand, within b3022at 3166.357 kb on - strand, within b3022at 3166.370 kb on + strand, within b3022at 3166.376 kb on - strand, within b3022at 3166.377 kb on + strand, within b3022at 3166.382 kb on + strand, within b3022at 3166.382 kb on + strand, within b3022at 3166.388 kb on - strand, within b3022at 3166.388 kb on - strand, within b3022at 3166.390 kb on - strand, within b3022at 3166.420 kb on - strand, within b3022at 3166.441 kb on - strand, within b3022at 3166.480 kb on - strand, within b3022at 3166.480 kb on - strand, within b3022at 3166.523 kb on + strand, within b3022at 3166.539 kb on + strandat 3166.551 kb on - strandat 3166.551 kb on - strandat 3166.551 kb on - strandat 3166.576 kb on - strandat 3166.576 kb on - strandat 3166.696 kb on - strandat 3166.703 kb on + strandat 3166.703 kb on + strandat 3166.771 kb on - strandat 3166.795 kb on - strandat 3166.795 kb on - strandat 3166.843 kb on + strand, within ygiVat 3166.909 kb on - strand, within ygiVat 3166.956 kb on - strand, within ygiVat 3166.956 kb on - strand, within ygiVat 3166.989 kb on + strand, within ygiVat 3166.989 kb on + strand, within ygiVat 3167.060 kb on + strand, within ygiVat 3167.154 kb on + strand, within ygiVat 3167.158 kb on + strand, within ygiVat 3167.175 kb on + strand, within ygiVat 3167.175 kb on + strand, within ygiVat 3167.175 kb on + strand, within ygiVat 3167.192 kb on + strand, within ygiVat 3167.227 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin Time24hr
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3,164,942 + ygiS b3020 0.50 +0.4
3,164,953 - ygiS b3020 0.51 +0.1
3,164,953 - ygiS b3020 0.51 +0.1
3,164,984 - ygiS b3020 0.53 +0.1
3,164,984 - ygiS b3020 0.53 -0.3
3,164,984 - ygiS b3020 0.53 +0.4
3,165,101 + ygiS b3020 0.60 +0.2
3,165,112 + ygiS b3020 0.61 -0.9
3,165,112 + ygiS b3020 0.61 -0.2
3,165,114 + ygiS b3020 0.61 +0.1
3,165,122 - ygiS b3020 0.62 +0.3
3,165,147 - ygiS b3020 0.63 -0.1
3,165,147 - ygiS b3020 0.63 -0.1
3,165,196 - ygiS b3020 0.66 -0.0
3,165,256 - ygiS b3020 0.70 +0.3
3,165,256 - ygiS b3020 0.70 +0.2
3,165,304 + ygiS b3020 0.73 +0.0
3,165,442 + ygiS b3020 0.81 +0.3
3,165,443 - ygiS b3020 0.81 +0.0
3,165,477 + ygiS b3020 0.84 -0.2
3,165,516 - ygiS b3020 0.86 +0.2
3,165,530 - ygiS b3020 0.87 +0.0
3,165,530 - ygiS b3020 0.87 +0.5
3,165,544 + ygiS b3020 0.88 -0.0
3,165,545 - ygiS b3020 0.88 +0.3
3,165,545 - ygiS b3020 0.88 +0.0
3,165,552 - ygiS b3020 0.88 +0.1
3,165,574 + ygiS b3020 0.90 +0.0
3,165,583 - -0.2
3,165,583 - -0.2
3,165,616 - +0.4
3,165,667 - +0.6
3,165,682 + -0.1
3,165,682 + -0.1
3,165,687 - -0.1
3,165,692 + +0.6
3,165,692 - +0.7
3,165,692 - +0.4
3,165,700 + +0.2
3,165,736 + +0.1
3,165,745 - -0.2
3,165,748 - +0.5
3,165,770 + +0.1
3,165,775 + +2.1
3,165,784 - +0.6
3,165,798 + +0.3
3,165,798 + +0.5
3,165,822 + +0.8
3,165,822 + +0.2
3,165,895 + +1.6
3,165,903 + +2.2
3,165,914 - ygiT b3021 0.10 +0.8
3,165,946 - ygiT b3021 0.18 -1.7
3,165,950 - ygiT b3021 0.19 -0.1
3,165,950 - ygiT b3021 0.19 +1.1
3,165,985 - ygiT b3021 0.28 +0.1
3,166,018 - ygiT b3021 0.37 +0.9
3,166,093 + ygiT b3021 0.56 +0.6
3,166,285 - -0.1
3,166,285 - +0.1
3,166,286 - +0.0
3,166,290 - +0.7
3,166,290 - -0.0
3,166,299 - +0.1
3,166,308 - b3022 b3022 0.13 +0.3
3,166,308 - b3022 b3022 0.13 +0.3
3,166,325 - b3022 b3022 0.19 +0.1
3,166,330 - b3022 b3022 0.20 +0.4
3,166,330 - b3022 b3022 0.20 +0.3
3,166,335 - b3022 b3022 0.22 -0.2
3,166,337 + b3022 b3022 0.23 +0.6
3,166,345 - b3022 b3022 0.25 +0.1
3,166,345 - b3022 b3022 0.25 +0.1
3,166,346 - b3022 b3022 0.26 +0.0
3,166,357 - b3022 b3022 0.29 +0.3
3,166,370 + b3022 b3022 0.34 -0.7
3,166,376 - b3022 b3022 0.36 +0.3
3,166,377 + b3022 b3022 0.36 +0.3
3,166,382 + b3022 b3022 0.38 -0.0
3,166,382 + b3022 b3022 0.38 +0.6
3,166,388 - b3022 b3022 0.40 -0.1
3,166,388 - b3022 b3022 0.40 +0.2
3,166,390 - b3022 b3022 0.40 +0.4
3,166,420 - b3022 b3022 0.51 +0.3
3,166,441 - b3022 b3022 0.58 +0.0
3,166,480 - b3022 b3022 0.71 -0.0
3,166,480 - b3022 b3022 0.71 +0.3
3,166,523 + b3022 b3022 0.85 +0.5
3,166,539 + -0.1
3,166,551 - -0.1
3,166,551 - +0.1
3,166,551 - +0.2
3,166,576 - +0.4
3,166,576 - +0.1
3,166,696 - +0.2
3,166,703 + +0.1
3,166,703 + -0.2
3,166,771 - +0.2
3,166,795 - +0.8
3,166,795 - +0.2
3,166,843 + ygiV b3023 0.15 -0.2
3,166,909 - ygiV b3023 0.29 -0.1
3,166,956 - ygiV b3023 0.38 +0.2
3,166,956 - ygiV b3023 0.38 +0.1
3,166,989 + ygiV b3023 0.45 +0.0
3,166,989 + ygiV b3023 0.45 +0.3
3,167,060 + ygiV b3023 0.60 +0.2
3,167,154 + ygiV b3023 0.79 +0.2
3,167,158 + ygiV b3023 0.80 +0.1
3,167,175 + ygiV b3023 0.84 +0.6
3,167,175 + ygiV b3023 0.84 -0.4
3,167,175 + ygiV b3023 0.84 +0.0
3,167,192 + ygiV b3023 0.87 +0.4
3,167,227 + -0.0

Or see this region's nucleotide sequence