Strain Fitness in Escherichia coli BW25113 around b3006

Experiment: LB + carbenicillin Time24hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyghA and exbD are separated by 271 nucleotidesexbD and exbB are separated by 6 nucleotidesexbB and metC are separated by 251 nucleotides b3003: yghA - oxidoreductase (NCBI), at 3,147,684 to 3,148,568 yghA b3005: exbD - membrane spanning protein in TonB-ExbB-ExbD complex (NCBI), at 3,148,840 to 3,149,265 exbD b3006: exbB - membrane spanning protein in TonB-ExbB-ExbD complex (NCBI), at 3,149,272 to 3,150,006 exbB b3008: metC - cystathionine beta-lyase (NCBI), at 3,150,258 to 3,151,445 metC Position (kb) 3149 3150 3151Strain fitness (log2 ratio) -1 0 1at 3148.282 kb on - strand, within yghAat 3148.282 kb on - strand, within yghAat 3148.299 kb on + strand, within yghAat 3148.299 kb on + strand, within yghAat 3148.371 kb on + strand, within yghAat 3148.384 kb on - strand, within yghAat 3148.384 kb on - strand, within yghAat 3148.386 kb on + strand, within yghAat 3148.386 kb on + strand, within yghAat 3148.387 kb on - strand, within yghAat 3148.387 kb on - strand, within yghAat 3148.515 kb on + strandat 3148.519 kb on + strandat 3148.540 kb on - strandat 3148.552 kb on - strandat 3148.611 kb on - strandat 3148.694 kb on + strandat 3148.700 kb on - strandat 3148.758 kb on - strandat 3148.758 kb on - strandat 3148.875 kb on - strandat 3148.906 kb on + strand, within exbDat 3148.936 kb on - strand, within exbDat 3148.992 kb on - strand, within exbDat 3149.036 kb on + strand, within exbDat 3149.092 kb on + strand, within exbDat 3149.120 kb on - strandat 3149.120 kb on - strand, within exbDat 3149.131 kb on + strand, within exbDat 3149.131 kb on + strand, within exbDat 3149.208 kb on + strand, within exbDat 3149.377 kb on + strand, within exbBat 3149.377 kb on + strand, within exbBat 3149.380 kb on + strand, within exbBat 3149.396 kb on - strand, within exbBat 3149.448 kb on - strand, within exbBat 3149.501 kb on + strand, within exbBat 3149.508 kb on + strand, within exbBat 3149.543 kb on - strand, within exbBat 3149.553 kb on - strand, within exbBat 3149.554 kb on + strand, within exbBat 3149.564 kb on + strand, within exbBat 3149.659 kb on + strand, within exbBat 3149.682 kb on - strand, within exbBat 3149.682 kb on - strand, within exbBat 3149.690 kb on - strand, within exbBat 3149.707 kb on + strand, within exbBat 3149.710 kb on - strand, within exbBat 3149.718 kb on + strand, within exbBat 3149.803 kb on - strand, within exbBat 3149.803 kb on - strand, within exbBat 3149.866 kb on + strand, within exbBat 3149.956 kb on + strandat 3149.956 kb on + strandat 3149.956 kb on + strandat 3149.983 kb on + strandat 3150.016 kb on - strandat 3150.257 kb on - strandat 3150.288 kb on - strandat 3150.292 kb on + strandat 3150.300 kb on - strandat 3150.300 kb on - strandat 3150.337 kb on - strandat 3150.378 kb on - strand, within metCat 3150.421 kb on + strand, within metCat 3150.421 kb on + strand, within metCat 3150.514 kb on - strand, within metCat 3150.575 kb on + strand, within metCat 3150.577 kb on - strand, within metCat 3150.583 kb on - strand, within metCat 3150.640 kb on - strand, within metCat 3150.654 kb on + strand, within metCat 3150.669 kb on + strand, within metCat 3150.706 kb on - strand, within metCat 3150.791 kb on - strand, within metCat 3150.820 kb on + strand, within metCat 3150.820 kb on + strand, within metCat 3150.912 kb on + strand, within metCat 3150.914 kb on + strand, within metCat 3150.915 kb on + strand, within metCat 3150.932 kb on - strand, within metC

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin Time24hr
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3,148,282 - yghA b3003 0.68 +0.0
3,148,282 - yghA b3003 0.68 -0.1
3,148,299 + yghA b3003 0.69 +0.2
3,148,299 + yghA b3003 0.69 +0.5
3,148,371 + yghA b3003 0.78 -0.4
3,148,384 - yghA b3003 0.79 -0.2
3,148,384 - yghA b3003 0.79 +0.1
3,148,386 + yghA b3003 0.79 -0.0
3,148,386 + yghA b3003 0.79 -0.5
3,148,387 - yghA b3003 0.79 +0.5
3,148,387 - yghA b3003 0.79 +0.4
3,148,515 + -0.4
3,148,519 + +0.4
3,148,540 - +0.1
3,148,552 - +0.2
3,148,611 - -0.3
3,148,694 + +0.1
3,148,700 - +0.2
3,148,758 - +0.2
3,148,758 - +0.2
3,148,875 - +0.2
3,148,906 + exbD b3005 0.15 -0.3
3,148,936 - exbD b3005 0.23 +0.2
3,148,992 - exbD b3005 0.36 +0.2
3,149,036 + exbD b3005 0.46 -0.6
3,149,092 + exbD b3005 0.59 +0.1
3,149,120 - -0.0
3,149,120 - exbD b3005 0.66 -0.4
3,149,131 + exbD b3005 0.68 -0.1
3,149,131 + exbD b3005 0.68 -0.2
3,149,208 + exbD b3005 0.86 +0.2
3,149,377 + exbB b3006 0.14 +1.4
3,149,377 + exbB b3006 0.14 +0.4
3,149,380 + exbB b3006 0.15 -0.2
3,149,396 - exbB b3006 0.17 +0.4
3,149,448 - exbB b3006 0.24 +0.2
3,149,501 + exbB b3006 0.31 +0.4
3,149,508 + exbB b3006 0.32 +0.0
3,149,543 - exbB b3006 0.37 +0.5
3,149,553 - exbB b3006 0.38 +1.0
3,149,554 + exbB b3006 0.38 +0.4
3,149,564 + exbB b3006 0.40 +0.1
3,149,659 + exbB b3006 0.53 +0.4
3,149,682 - exbB b3006 0.56 +0.1
3,149,682 - exbB b3006 0.56 +0.9
3,149,690 - exbB b3006 0.57 +0.3
3,149,707 + exbB b3006 0.59 -0.1
3,149,710 - exbB b3006 0.60 +0.4
3,149,718 + exbB b3006 0.61 +0.0
3,149,803 - exbB b3006 0.72 +0.4
3,149,803 - exbB b3006 0.72 -0.1
3,149,866 + exbB b3006 0.81 +0.1
3,149,956 + -0.1
3,149,956 + +0.3
3,149,956 + +0.0
3,149,983 + +0.5
3,150,016 - +0.1
3,150,257 - +0.4
3,150,288 - -0.2
3,150,292 + -0.2
3,150,300 - -0.0
3,150,300 - +0.2
3,150,337 - +0.2
3,150,378 - metC b3008 0.10 +0.3
3,150,421 + metC b3008 0.14 +0.1
3,150,421 + metC b3008 0.14 -0.3
3,150,514 - metC b3008 0.22 +0.6
3,150,575 + metC b3008 0.27 +0.0
3,150,577 - metC b3008 0.27 +0.6
3,150,583 - metC b3008 0.27 +1.0
3,150,640 - metC b3008 0.32 +0.1
3,150,654 + metC b3008 0.33 +0.3
3,150,669 + metC b3008 0.35 +0.0
3,150,706 - metC b3008 0.38 +0.1
3,150,791 - metC b3008 0.45 -0.3
3,150,820 + metC b3008 0.47 -0.5
3,150,820 + metC b3008 0.47 +0.2
3,150,912 + metC b3008 0.55 +0.3
3,150,914 + metC b3008 0.55 -0.1
3,150,915 + metC b3008 0.55 -0.1
3,150,932 - metC b3008 0.57 -0.4

Or see this region's nucleotide sequence