Strain Fitness in Escherichia coli BW25113 around b1357

Experiment: LB + carbenicillin Time24hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntb1355 and racR are separated by 308 nucleotidesracR and ydaS are separated by 123 nucleotidesydaS and ydaT are separated by 22 nucleotidesydaT and ydaU are separated by 12 nucleotides b1355: b1355 - orf, hypothetical protein (VIMSS), at 1,417,346 to 1,417,480 b1355 b1356: racR - Rac prophage; predicted DNA-binding transcriptional regulator (NCBI), at 1,417,789 to 1,418,265 racR b1357: ydaS - Rac prophage; predicted DNA-binding transcriptional regulator (NCBI), at 1,418,389 to 1,418,685 ydaS b1358: ydaT - Rac prophage; predicted protein (NCBI), at 1,418,708 to 1,419,130 ydaT b1359: ydaU - Rac prophage; conserved protein (NCBI), at 1,419,143 to 1,420,000 ydaU Position (kb) 1418 1419Strain fitness (log2 ratio) -1 0 1at 1417.427 kb on + strand, within b1355at 1417.431 kb on - strand, within b1355at 1417.459 kb on + strand, within b1355at 1417.518 kb on - strandat 1417.574 kb on + strandat 1418.573 kb on - strand, within ydaSat 1418.619 kb on - strand, within ydaSat 1418.746 kb on - strandat 1418.848 kb on - strand, within ydaTat 1418.848 kb on - strand, within ydaTat 1419.186 kb on + strandat 1419.186 kb on + strandat 1419.235 kb on - strand, within ydaUat 1419.360 kb on - strand, within ydaUat 1419.393 kb on - strand, within ydaUat 1419.393 kb on - strand, within ydaU

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin Time24hr
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1,417,427 + b1355 b1355 0.60 -0.3
1,417,431 - b1355 b1355 0.63 +0.0
1,417,459 + b1355 b1355 0.84 +0.6
1,417,518 - +0.1
1,417,574 + +0.1
1,418,573 - ydaS b1357 0.62 +0.4
1,418,619 - ydaS b1357 0.77 +0.5
1,418,746 - +0.1
1,418,848 - ydaT b1358 0.33 +0.3
1,418,848 - ydaT b1358 0.33 -0.6
1,419,186 + +0.8
1,419,186 + +0.1
1,419,235 - ydaU b1359 0.11 -0.3
1,419,360 - ydaU b1359 0.25 +0.2
1,419,393 - ydaU b1359 0.29 +0.2
1,419,393 - ydaU b1359 0.29 +0.1

Or see this region's nucleotide sequence