Experiment: LB + carbenicillin Time24hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lar and recT are separated by 56 nucleotides recT and recE overlap by 8 nucleotides recE and racC are separated by 101 nucleotides racC and ydaE are separated by 74 nucleotides ydaE and ydaD overlap by 1 nucleotides
b1348: lar - Rac prophage; restriction alleviation protein (NCBI), at 1,411,757 to 1,411,951
lar
b1349: recT - Rac prophage; recombination and repair protein (NCBI), at 1,412,008 to 1,412,817
recT
b1350: recE - Rac prophage; exonuclease VIII, 5' -> 3' specific dsDNA exonuclease (NCBI), at 1,412,810 to 1,415,410
recE
b1351: racC - Rac prophage; predicted protein (NCBI), at 1,415,512 to 1,415,787
racC
b4526: ydaE - Rac prophage; conserved protein (NCBI), at 1,415,862 to 1,416,032
ydaE
b1352: ydaD - orf, hypothetical protein (VIMSS), at 1,416,032 to 1,416,253
ydaD
Position (kb)
1412
1413
1414
1415
1416 Strain fitness (log2 ratio)
-1
0
1
2 at 1411.844 kb on - strand, within lar at 1411.855 kb on - strand, within lar at 1411.865 kb on - strand, within lar at 1411.865 kb on - strand, within lar at 1411.867 kb on - strand, within lar at 1411.880 kb on - strand, within lar at 1411.880 kb on - strand, within lar at 1412.066 kb on - strand at 1412.069 kb on - strand at 1412.069 kb on - strand at 1412.080 kb on - strand at 1412.090 kb on - strand, within recT at 1412.103 kb on - strand, within recT at 1412.103 kb on - strand, within recT at 1412.113 kb on - strand, within recT at 1412.192 kb on - strand, within recT at 1412.213 kb on - strand, within recT at 1412.213 kb on - strand, within recT at 1412.235 kb on - strand, within recT at 1412.302 kb on - strand, within recT at 1412.302 kb on - strand, within recT at 1412.538 kb on - strand, within recT at 1412.896 kb on + strand at 1412.896 kb on + strand at 1412.896 kb on - strand at 1412.896 kb on - strand at 1413.042 kb on + strand at 1413.054 kb on + strand at 1413.054 kb on + strand at 1413.135 kb on + strand, within recE at 1413.341 kb on + strand, within recE at 1413.411 kb on + strand, within recE at 1413.411 kb on + strand, within recE at 1413.755 kb on - strand, within recE at 1413.755 kb on - strand, within recE at 1413.958 kb on - strand, within recE at 1413.977 kb on - strand, within recE at 1414.203 kb on + strand, within recE at 1414.203 kb on + strand, within recE at 1414.291 kb on - strand, within recE at 1414.310 kb on - strand, within recE at 1414.602 kb on - strand, within recE at 1414.633 kb on - strand, within recE at 1414.633 kb on - strand, within recE at 1414.719 kb on - strand, within recE at 1414.747 kb on + strand, within recE at 1414.758 kb on + strand, within recE at 1414.758 kb on + strand, within recE at 1415.046 kb on + strand, within recE at 1415.106 kb on + strand, within recE at 1415.187 kb on + strand at 1415.187 kb on + strand at 1415.195 kb on - strand at 1415.195 kb on - strand at 1415.195 kb on - strand at 1415.300 kb on + strand at 1415.416 kb on - strand at 1415.468 kb on + strand at 1415.468 kb on + strand at 1415.468 kb on + strand at 1415.468 kb on + strand at 1415.468 kb on + strand at 1415.468 kb on + strand at 1415.484 kb on + strand at 1415.538 kb on + strand at 1415.620 kb on + strand, within racC at 1415.625 kb on - strand, within racC at 1415.649 kb on - strand, within racC
Per-strain Table
Position Strand Gene LocusTag Fraction LB + carbenicillin Time24hr remove 1,411,844 - lar b1348 0.45 +0.2 1,411,855 - lar b1348 0.50 +0.7 1,411,865 - lar b1348 0.55 +0.2 1,411,865 - lar b1348 0.55 +1.9 1,411,867 - lar b1348 0.56 +1.2 1,411,880 - lar b1348 0.63 -0.2 1,411,880 - lar b1348 0.63 +0.8 1,412,066 - +0.0 1,412,069 - +0.6 1,412,069 - +0.6 1,412,080 - +0.3 1,412,090 - recT b1349 0.10 +0.3 1,412,103 - recT b1349 0.12 +0.2 1,412,103 - recT b1349 0.12 -0.1 1,412,113 - recT b1349 0.13 +0.6 1,412,192 - recT b1349 0.23 +0.4 1,412,213 - recT b1349 0.25 -0.1 1,412,213 - recT b1349 0.25 +0.1 1,412,235 - recT b1349 0.28 +0.1 1,412,302 - recT b1349 0.36 +0.2 1,412,302 - recT b1349 0.36 +0.5 1,412,538 - recT b1349 0.65 +0.5 1,412,896 + +1.7 1,412,896 + -0.8 1,412,896 - +0.4 1,412,896 - -0.0 1,413,042 + +0.6 1,413,054 + +0.4 1,413,054 + +0.6 1,413,135 + recE b1350 0.12 +0.6 1,413,341 + recE b1350 0.20 +0.6 1,413,411 + recE b1350 0.23 +0.4 1,413,411 + recE b1350 0.23 +0.4 1,413,755 - recE b1350 0.36 +1.3 1,413,755 - recE b1350 0.36 +0.2 1,413,958 - recE b1350 0.44 +1.4 1,413,977 - recE b1350 0.45 +0.7 1,414,203 + recE b1350 0.54 -0.0 1,414,203 + recE b1350 0.54 +0.1 1,414,291 - recE b1350 0.57 +0.6 1,414,310 - recE b1350 0.58 +0.6 1,414,602 - recE b1350 0.69 +0.5 1,414,633 - recE b1350 0.70 -1.0 1,414,633 - recE b1350 0.70 +0.9 1,414,719 - recE b1350 0.73 +0.2 1,414,747 + recE b1350 0.74 +0.5 1,414,758 + recE b1350 0.75 +0.4 1,414,758 + recE b1350 0.75 +0.2 1,415,046 + recE b1350 0.86 +0.4 1,415,106 + recE b1350 0.88 +0.3 1,415,187 + +0.3 1,415,187 + +0.4 1,415,195 - +0.3 1,415,195 - -0.3 1,415,195 - +0.5 1,415,300 + -0.0 1,415,416 - +0.4 1,415,468 + +0.2 1,415,468 + -0.5 1,415,468 + -0.0 1,415,468 + -0.1 1,415,468 + +0.1 1,415,468 + -0.5 1,415,484 + -0.1 1,415,538 + +0.4 1,415,620 + racC b1351 0.39 +0.4 1,415,625 - racC b1351 0.41 +1.4 1,415,649 - racC b1351 0.50 +0.4
Or see this region's nucleotide sequence