Experiment: LB + carbenicillin Time24hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt metN and gmhB are separated by 187 nucleotides gmhB and rrsH are separated by 362 nucleotides
b0199: metN - DL-methionine transporter subunit (NCBI), at 221,614 to 222,645
metN
b0200: gmhB - hypothetical protein (NCBI), at 222,833 to 223,408
gmhB
b0201: rrsH - 16S ribosomal RNA (NCBI), at 223,771 to 225,312
rrsH
Position (kb) 222
223
224 Strain fitness (log2 ratio) -2
-1
0
1 at 221.846 kb on + strand, within metN at 221.847 kb on + strand, within metN at 221.848 kb on + strand, within metN at 221.856 kb on + strand, within metN at 221.911 kb on - strand, within metN at 221.967 kb on + strand, within metN at 221.967 kb on + strand, within metN at 221.975 kb on - strand, within metN at 221.999 kb on - strand, within metN at 222.001 kb on + strand, within metN at 222.009 kb on - strand, within metN at 222.027 kb on - strand, within metN at 222.061 kb on - strand, within metN at 222.102 kb on - strand, within metN at 222.102 kb on - strand, within metN at 222.159 kb on + strand, within metN at 222.203 kb on + strand, within metN at 222.239 kb on + strand, within metN at 222.239 kb on + strand, within metN at 222.247 kb on + strand, within metN at 222.269 kb on - strand, within metN at 222.279 kb on - strand, within metN at 222.364 kb on - strand, within metN at 222.425 kb on - strand, within metN at 222.495 kb on - strand, within metN at 222.501 kb on + strand, within metN at 222.501 kb on + strand, within metN at 222.507 kb on - strand, within metN at 222.511 kb on + strand, within metN at 222.520 kb on - strand, within metN at 222.578 kb on - strand at 222.588 kb on - strand at 222.588 kb on - strand at 222.594 kb on - strand at 222.594 kb on - strand at 222.620 kb on - strand at 222.731 kb on - strand at 222.732 kb on + strand at 222.732 kb on + strand at 222.813 kb on + strand at 222.907 kb on - strand, within gmhB at 222.907 kb on - strand, within gmhB at 222.941 kb on - strand, within gmhB at 223.224 kb on + strand, within gmhB at 223.232 kb on - strand, within gmhB at 223.232 kb on - strand, within gmhB at 223.477 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB + carbenicillin Time24hr remove 221,846 + metN b0199 0.22 -0.1 221,847 + metN b0199 0.23 +0.4 221,848 + metN b0199 0.23 +0.1 221,856 + metN b0199 0.23 +0.1 221,911 - metN b0199 0.29 +0.4 221,967 + metN b0199 0.34 +0.0 221,967 + metN b0199 0.34 -0.1 221,975 - metN b0199 0.35 +0.4 221,999 - metN b0199 0.37 +0.2 222,001 + metN b0199 0.38 -0.4 222,009 - metN b0199 0.38 -0.3 222,027 - metN b0199 0.40 +0.4 222,061 - metN b0199 0.43 +0.2 222,102 - metN b0199 0.47 -0.1 222,102 - metN b0199 0.47 -0.0 222,159 + metN b0199 0.53 +0.0 222,203 + metN b0199 0.57 +0.1 222,239 + metN b0199 0.61 +0.4 222,239 + metN b0199 0.61 +0.1 222,247 + metN b0199 0.61 +0.2 222,269 - metN b0199 0.63 +0.1 222,279 - metN b0199 0.64 +0.4 222,364 - metN b0199 0.73 +0.0 222,425 - metN b0199 0.79 +0.3 222,495 - metN b0199 0.85 -0.4 222,501 + metN b0199 0.86 +0.3 222,501 + metN b0199 0.86 +0.6 222,507 - metN b0199 0.87 +0.6 222,511 + metN b0199 0.87 +0.1 222,520 - metN b0199 0.88 +0.1 222,578 - +0.4 222,588 - +0.2 222,588 - -0.3 222,594 - +0.7 222,594 - +0.8 222,620 - -0.1 222,731 - +0.0 222,732 + +0.4 222,732 + +0.1 222,813 + +0.1 222,907 - gmhB b0200 0.13 -0.5 222,907 - gmhB b0200 0.13 +0.1 222,941 - gmhB b0200 0.19 -0.2 223,224 + gmhB b0200 0.68 +1.5 223,232 - gmhB b0200 0.69 -2.1 223,232 - gmhB b0200 0.69 +1.5 223,477 - -0.3
Or see this region's nucleotide sequence