Experiment: LB + ciprofloxacin Time9hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rrlC and rrfC are separated by 92 nucleotides rrfC and aspT are separated by 52 nucleotides aspT and trpT are separated by 8 nucleotides trpT and hdfR are separated by 95 nucleotides hdfR and yifE are separated by 118 nucleotides yifE and yifB are separated by 24 nucleotides
b3758: rrlC - 23S ribosomal RNA (NCBI), at 3,941,727 to 3,944,630
rrlC
b3759: rrfC - 5S ribosomal RNA (NCBI), at 3,944,723 to 3,944,842
rrfC
b3760: aspT - tRNA-Asp (NCBI), at 3,944,895 to 3,944,971
aspT
b3761: trpT - tRNA-Trp (NCBI), at 3,944,980 to 3,945,055
trpT
b4480: hdfR - transcriptional regulator HdfR (NCBI), at 3,945,151 to 3,945,990
hdfR
b3764: yifE - hypothetical protein (NCBI), at 3,946,109 to 3,946,447
yifE
b3765: yifB - predicted bifunctional enzyme and transcriptional regulator (RefSeq), at 3,946,472 to 3,947,992
yifB
Position (kb)
3945
3946 Strain fitness (log2 ratio)
-1
0
1 at 3944.961 kb on + strand, within aspT at 3944.971 kb on + strand at 3945.046 kb on + strand, within trpT at 3945.298 kb on + strand, within hdfR at 3945.298 kb on + strand, within hdfR at 3945.322 kb on - strand, within hdfR at 3945.322 kb on - strand, within hdfR at 3945.390 kb on - strand, within hdfR at 3945.392 kb on + strand, within hdfR at 3945.392 kb on + strand, within hdfR at 3945.402 kb on - strand, within hdfR at 3945.402 kb on - strand, within hdfR at 3945.543 kb on + strand, within hdfR at 3945.543 kb on + strand, within hdfR at 3945.551 kb on - strand, within hdfR at 3945.744 kb on - strand, within hdfR at 3945.783 kb on + strand, within hdfR at 3945.783 kb on + strand, within hdfR at 3945.897 kb on + strand, within hdfR at 3945.902 kb on + strand, within hdfR at 3945.914 kb on - strand at 3946.030 kb on - strand at 3946.030 kb on - strand at 3946.045 kb on + strand at 3946.056 kb on + strand at 3946.058 kb on + strand at 3946.064 kb on - strand at 3946.071 kb on + strand at 3946.102 kb on + strand at 3946.102 kb on + strand at 3946.127 kb on - strand at 3946.176 kb on - strand, within yifE at 3946.179 kb on - strand, within yifE at 3946.200 kb on + strand, within yifE at 3946.281 kb on - strand, within yifE at 3946.281 kb on - strand, within yifE at 3946.295 kb on - strand, within yifE at 3946.309 kb on + strand, within yifE at 3946.345 kb on + strand, within yifE at 3946.360 kb on + strand, within yifE at 3946.360 kb on + strand, within yifE at 3946.366 kb on - strand, within yifE at 3946.366 kb on - strand, within yifE at 3946.525 kb on + strand at 3946.528 kb on - strand at 3946.528 kb on - strand at 3946.575 kb on - strand at 3946.575 kb on - strand at 3946.621 kb on - strand at 3946.621 kb on - strand at 3946.628 kb on + strand, within yifB at 3946.632 kb on + strand, within yifB at 3946.706 kb on + strand, within yifB at 3946.761 kb on + strand, within yifB at 3946.883 kb on - strand, within yifB at 3946.903 kb on - strand, within yifB
Per-strain Table
Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time9hr remove 3,944,961 + aspT b3760 0.86 -0.4 3,944,971 + -0.9 3,945,046 + trpT b3761 0.87 -0.1 3,945,298 + hdfR b4480 0.17 +0.8 3,945,298 + hdfR b4480 0.17 -0.5 3,945,322 - hdfR b4480 0.20 +0.7 3,945,322 - hdfR b4480 0.20 +1.2 3,945,390 - hdfR b4480 0.28 +0.5 3,945,392 + hdfR b4480 0.29 +0.9 3,945,392 + hdfR b4480 0.29 +0.5 3,945,402 - hdfR b4480 0.30 +0.3 3,945,402 - hdfR b4480 0.30 -0.5 3,945,543 + hdfR b4480 0.47 +0.4 3,945,543 + hdfR b4480 0.47 +0.0 3,945,551 - hdfR b4480 0.48 +0.5 3,945,744 - hdfR b4480 0.71 +1.3 3,945,783 + hdfR b4480 0.75 -0.1 3,945,783 + hdfR b4480 0.75 +0.5 3,945,897 + hdfR b4480 0.89 +0.9 3,945,902 + hdfR b4480 0.89 +0.2 3,945,914 - +0.8 3,946,030 - +0.5 3,946,030 - +0.4 3,946,045 + -0.0 3,946,056 + +0.3 3,946,058 + +0.1 3,946,064 - +0.6 3,946,071 + +0.6 3,946,102 + +0.4 3,946,102 + +0.2 3,946,127 - -0.0 3,946,176 - yifE b3764 0.20 +0.1 3,946,179 - yifE b3764 0.21 -0.5 3,946,200 + yifE b3764 0.27 +0.2 3,946,281 - yifE b3764 0.51 +0.9 3,946,281 - yifE b3764 0.51 +0.1 3,946,295 - yifE b3764 0.55 +0.3 3,946,309 + yifE b3764 0.59 +0.6 3,946,345 + yifE b3764 0.70 -1.0 3,946,360 + yifE b3764 0.74 +0.1 3,946,360 + yifE b3764 0.74 +0.2 3,946,366 - yifE b3764 0.76 +0.4 3,946,366 - yifE b3764 0.76 +0.7 3,946,525 + +0.2 3,946,528 - -0.1 3,946,528 - +0.4 3,946,575 - +0.4 3,946,575 - +0.2 3,946,621 - +0.3 3,946,621 - +0.2 3,946,628 + yifB b3765 0.10 +0.1 3,946,632 + yifB b3765 0.11 -0.2 3,946,706 + yifB b3765 0.15 +0.5 3,946,761 + yifB b3765 0.19 +0.0 3,946,883 - yifB b3765 0.27 +0.1 3,946,903 - yifB b3765 0.28 +0.4
Or see this region's nucleotide sequence