Experiment: LB + ciprofloxacin Time9hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt intE and ymfG overlap by 20 nucleotides ymfG and ymfI are separated by 464 nucleotides ymfI and ymfJ overlap by 63 nucleotides ymfJ and ymfK are separated by 174 nucleotides ymfK and b1146 are separated by 90 nucleotides
b1140: intE - e14 prophage; predicted integrase (NCBI), at 1,198,902 to 1,200,029
intE
b1141: ymfG - e14 prophage; predicted excisionase (NCBI), at 1,200,010 to 1,200,255
ymfG
b1143: ymfI - orf, hypothetical protein (VIMSS), at 1,200,720 to 1,201,061
ymfI
b1144: ymfJ - orf, hypothetical protein (VIMSS), at 1,200,999 to 1,201,307
ymfJ
b1145: ymfK - e14 prophage; repressor protein phage e14 (NCBI), at 1,201,482 to 1,202,156
ymfK
b1146: b1146 - orf, hypothetical protein (VIMSS), at 1,202,247 to 1,202,447
b1146
Position (kb)
1200
1201
1202 Strain fitness (log2 ratio)
-1
0
1 at 1200.021 kb on + strand at 1200.088 kb on + strand, within ymfG at 1200.127 kb on - strand, within ymfG at 1200.191 kb on - strand, within ymfG at 1200.236 kb on + strand at 1200.278 kb on - strand at 1200.298 kb on + strand at 1200.340 kb on - strand at 1200.347 kb on + strand at 1200.360 kb on - strand at 1200.368 kb on - strand at 1200.404 kb on - strand at 1200.475 kb on - strand at 1200.475 kb on - strand at 1200.482 kb on + strand at 1200.683 kb on - strand at 1200.770 kb on - strand, within ymfI at 1200.919 kb on + strand, within ymfI at 1201.109 kb on - strand, within ymfJ at 1201.127 kb on + strand, within ymfJ at 1201.127 kb on + strand, within ymfJ at 1202.248 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time9hr remove 1,200,021 + +0.2 1,200,088 + ymfG b1141 0.32 -0.8 1,200,127 - ymfG b1141 0.48 +0.2 1,200,191 - ymfG b1141 0.74 +0.3 1,200,236 + -0.3 1,200,278 - -0.5 1,200,298 + +0.4 1,200,340 - +0.3 1,200,347 + +0.0 1,200,360 - -0.8 1,200,368 - -0.3 1,200,404 - +0.1 1,200,475 - +0.4 1,200,475 - -0.2 1,200,482 + -0.1 1,200,683 - +0.4 1,200,770 - ymfI b1143 0.15 -0.0 1,200,919 + ymfI b1143 0.58 +0.4 1,201,109 - ymfJ b1144 0.36 +0.4 1,201,127 + ymfJ b1144 0.41 +0.8 1,201,127 + ymfJ b1144 0.41 +0.3 1,202,248 - +0.4
Or see this region's nucleotide sequence