Strain Fitness in Escherichia coli BW25113 around b2820

Experiment: LB Time9hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrecD and recB overlap by 1 nucleotidesrecB and ptr overlap by 8 nucleotides b2819: recD - exonuclease V (RecBCD complex), alpha chain (NCBI), at 2,948,657 to 2,950,483 recD b2820: recB - exonuclease V (RecBCD complex), beta subunit (NCBI), at 2,950,483 to 2,954,025 recB b2821: ptr - protease III (NCBI), at 2,954,018 to 2,956,906 ptr Position (kb) 2950 2951 2952 2953 2954 2955Strain fitness (log2 ratio) -1 0 1at 2949.559 kb on - strand, within recDat 2949.564 kb on - strand, within recDat 2949.648 kb on + strand, within recDat 2949.648 kb on - strand, within recDat 2949.648 kb on - strand, within recDat 2949.658 kb on + strand, within recDat 2949.691 kb on - strand, within recDat 2949.691 kb on - strand, within recDat 2949.911 kb on - strand, within recDat 2950.492 kb on + strandat 2950.492 kb on + strandat 2950.500 kb on - strandat 2951.352 kb on - strand, within recBat 2951.941 kb on + strand, within recBat 2952.471 kb on - strand, within recBat 2952.820 kb on + strand, within recBat 2953.040 kb on - strand, within recBat 2953.978 kb on + strandat 2953.978 kb on + strandat 2954.006 kb on - strandat 2954.072 kb on + strandat 2954.072 kb on + strandat 2954.201 kb on - strandat 2954.359 kb on + strand, within ptrat 2954.508 kb on + strand, within ptrat 2954.516 kb on + strand, within ptrat 2954.587 kb on - strand, within ptrat 2954.617 kb on + strand, within ptrat 2954.617 kb on + strand, within ptrat 2954.626 kb on - strand, within ptrat 2954.626 kb on - strand, within ptrat 2954.713 kb on - strand, within ptrat 2954.724 kb on - strand, within ptrat 2954.759 kb on - strand, within ptrat 2954.759 kb on - strand, within ptrat 2954.771 kb on - strand, within ptrat 2954.771 kb on - strand, within ptrat 2954.777 kb on - strand, within ptrat 2954.782 kb on - strand, within ptrat 2954.782 kb on - strand, within ptrat 2954.857 kb on + strand, within ptrat 2954.857 kb on + strand, within ptrat 2954.905 kb on + strand, within ptrat 2954.905 kb on + strand, within ptrat 2954.985 kb on + strand, within ptrat 2954.985 kb on + strand, within ptr

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Time9hr
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2,949,559 - recD b2819 0.49 +0.5
2,949,564 - recD b2819 0.50 +0.0
2,949,648 + recD b2819 0.54 +0.2
2,949,648 - recD b2819 0.54 +0.2
2,949,648 - recD b2819 0.54 +0.5
2,949,658 + recD b2819 0.55 +0.2
2,949,691 - recD b2819 0.57 +0.8
2,949,691 - recD b2819 0.57 -0.2
2,949,911 - recD b2819 0.69 +0.1
2,950,492 + +0.1
2,950,492 + +0.2
2,950,500 - +0.4
2,951,352 - recB b2820 0.25 -0.6
2,951,941 + recB b2820 0.41 +0.4
2,952,471 - recB b2820 0.56 +0.0
2,952,820 + recB b2820 0.66 +0.3
2,953,040 - recB b2820 0.72 +1.0
2,953,978 + +0.5
2,953,978 + +0.0
2,954,006 - +0.0
2,954,072 + +0.6
2,954,072 + +0.4
2,954,201 - +0.4
2,954,359 + ptr b2821 0.12 +0.7
2,954,508 + ptr b2821 0.17 +0.7
2,954,516 + ptr b2821 0.17 +0.6
2,954,587 - ptr b2821 0.20 +0.4
2,954,617 + ptr b2821 0.21 -0.1
2,954,617 + ptr b2821 0.21 -0.6
2,954,626 - ptr b2821 0.21 +0.4
2,954,626 - ptr b2821 0.21 -0.1
2,954,713 - ptr b2821 0.24 +0.4
2,954,724 - ptr b2821 0.24 +0.4
2,954,759 - ptr b2821 0.26 -0.1
2,954,759 - ptr b2821 0.26 +0.1
2,954,771 - ptr b2821 0.26 -0.6
2,954,771 - ptr b2821 0.26 +0.3
2,954,777 - ptr b2821 0.26 +0.2
2,954,782 - ptr b2821 0.26 -0.1
2,954,782 - ptr b2821 0.26 +0.0
2,954,857 + ptr b2821 0.29 +0.1
2,954,857 + ptr b2821 0.29 -0.3
2,954,905 + ptr b2821 0.31 +0.6
2,954,905 + ptr b2821 0.31 -0.1
2,954,985 + ptr b2821 0.33 +0.1
2,954,985 + ptr b2821 0.33 +0.2

Or see this region's nucleotide sequence