Experiment: LB Time9hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dinB and yafN are separated by 51 nucleotides yafN and yafO are separated by 2 nucleotides yafO and yafP are separated by 9 nucleotides yafP and ykfJ are separated by 317 nucleotides ykfJ and prfH are separated by 16 nucleotides
b0231: dinB - DNA polymerase IV (NCBI), at 250,898 to 251,953
dinB
b0232: yafN - predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI), at 252,005 to 252,298
yafN
b0233: yafO - predicted toxin of the YafO-YafN toxin-antitoxin system (NCBI), at 252,301 to 252,699
yafO
b0234: yafP - predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI), at 252,709 to 253,161
yafP
b0235: ykfJ - hypothetical protein (RefSeq), at 253,479 to 253,685
ykfJ
b0236: prfH - peptide chain release factor 2 (NCBI), at 253,702 to 254,202
prfH
Position (kb) 252
253
254 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 251.803 kb on + strand, within dinB at 251.803 kb on + strand, within dinB at 251.818 kb on + strand, within dinB at 251.880 kb on + strand at 251.901 kb on + strand at 251.909 kb on - strand at 251.924 kb on + strand at 251.965 kb on - strand at 252.003 kb on - strand at 252.137 kb on - strand, within yafN at 252.155 kb on - strand, within yafN at 252.288 kb on + strand at 252.331 kb on + strand at 252.331 kb on + strand at 252.341 kb on - strand, within yafO at 252.346 kb on + strand, within yafO at 252.368 kb on - strand, within yafO at 252.368 kb on - strand, within yafO at 252.380 kb on + strand, within yafO at 252.381 kb on + strand, within yafO at 252.419 kb on + strand, within yafO at 252.427 kb on + strand, within yafO at 252.427 kb on + strand, within yafO at 252.440 kb on + strand, within yafO at 252.440 kb on + strand, within yafO at 252.440 kb on + strand, within yafO at 252.440 kb on + strand, within yafO at 252.440 kb on + strand, within yafO at 252.443 kb on - strand, within yafO at 252.475 kb on + strand, within yafO at 252.522 kb on - strand, within yafO at 252.522 kb on - strand, within yafO at 252.534 kb on - strand, within yafO at 252.538 kb on + strand, within yafO at 252.538 kb on + strand, within yafO at 252.563 kb on + strand, within yafO at 252.566 kb on - strand, within yafO at 252.574 kb on - strand, within yafO at 252.574 kb on - strand, within yafO at 252.592 kb on - strand, within yafO at 252.594 kb on - strand, within yafO at 252.597 kb on + strand, within yafO at 252.610 kb on - strand, within yafO at 252.614 kb on + strand, within yafO at 252.622 kb on - strand, within yafO at 252.628 kb on + strand, within yafO at 252.671 kb on + strand at 252.671 kb on + strand at 252.739 kb on - strand at 252.779 kb on - strand, within yafP at 252.781 kb on + strand, within yafP at 252.848 kb on + strand, within yafP at 252.849 kb on + strand, within yafP at 252.879 kb on + strand, within yafP at 252.896 kb on + strand, within yafP at 252.936 kb on + strand, within yafP at 252.973 kb on + strand, within yafP at 252.974 kb on + strand, within yafP at 253.041 kb on + strand, within yafP at 253.077 kb on + strand, within yafP at 253.087 kb on + strand, within yafP at 253.087 kb on + strand, within yafP at 253.087 kb on + strand, within yafP at 253.102 kb on + strand, within yafP at 253.188 kb on + strand at 253.198 kb on - strand at 253.220 kb on + strand at 253.222 kb on + strand at 253.222 kb on - strand at 253.272 kb on - strand at 253.275 kb on + strand at 253.283 kb on - strand at 253.301 kb on + strand at 253.350 kb on - strand at 253.350 kb on - strand at 253.350 kb on - strand at 253.350 kb on - strand at 253.376 kb on - strand at 253.381 kb on - strand at 253.383 kb on - strand at 253.386 kb on + strand at 253.387 kb on + strand at 253.394 kb on + strand at 253.394 kb on + strand at 253.396 kb on - strand at 253.422 kb on - strand at 253.422 kb on - strand at 253.423 kb on - strand at 253.423 kb on - strand at 253.427 kb on - strand at 253.428 kb on - strand at 253.430 kb on + strand at 253.430 kb on + strand at 253.430 kb on + strand at 253.430 kb on + strand at 253.432 kb on - strand at 253.432 kb on - strand at 253.434 kb on + strand at 253.437 kb on - strand at 253.438 kb on - strand at 253.438 kb on - strand at 253.438 kb on - strand at 253.438 kb on - strand at 253.450 kb on - strand at 253.454 kb on - strand at 253.491 kb on + strand at 253.504 kb on - strand, within ykfJ at 253.504 kb on - strand, within ykfJ at 253.520 kb on - strand, within ykfJ at 253.520 kb on - strand, within ykfJ at 253.520 kb on - strand, within ykfJ at 253.523 kb on + strand, within ykfJ at 253.533 kb on - strand, within ykfJ at 253.533 kb on - strand, within ykfJ at 253.533 kb on - strand, within ykfJ at 253.533 kb on - strand, within ykfJ at 253.535 kb on + strand, within ykfJ at 253.535 kb on + strand, within ykfJ at 253.543 kb on - strand, within ykfJ at 253.543 kb on - strand, within ykfJ at 253.543 kb on - strand, within ykfJ at 253.543 kb on - strand, within ykfJ at 253.543 kb on - strand, within ykfJ at 253.543 kb on - strand, within ykfJ at 253.543 kb on - strand, within ykfJ at 253.543 kb on - strand, within ykfJ at 253.557 kb on - strand, within ykfJ at 253.569 kb on - strand, within ykfJ at 253.571 kb on - strand, within ykfJ at 253.593 kb on + strand, within ykfJ at 253.603 kb on + strand, within ykfJ at 253.608 kb on - strand, within ykfJ at 253.617 kb on + strand, within ykfJ at 253.617 kb on + strand, within ykfJ at 253.617 kb on + strand, within ykfJ at 253.617 kb on + strand, within ykfJ at 253.625 kb on - strand, within ykfJ at 253.625 kb on - strand, within ykfJ at 253.625 kb on - strand, within ykfJ at 253.633 kb on - strand, within ykfJ at 253.637 kb on - strand, within ykfJ at 253.642 kb on - strand, within ykfJ at 253.642 kb on - strand, within ykfJ at 253.642 kb on - strand, within ykfJ at 253.681 kb on + strand at 253.727 kb on + strand at 253.728 kb on + strand at 253.728 kb on + strand at 253.728 kb on + strand at 253.734 kb on - strand at 253.734 kb on - strand at 253.735 kb on - strand at 253.735 kb on - strand at 253.736 kb on - strand at 253.736 kb on - strand at 253.736 kb on - strand at 253.747 kb on + strand at 253.765 kb on + strand, within prfH at 253.773 kb on + strand, within prfH at 253.805 kb on - strand, within prfH at 253.805 kb on - strand, within prfH at 253.838 kb on - strand, within prfH at 253.891 kb on - strand, within prfH
Per-strain Table
Position Strand Gene LocusTag Fraction LB Time9hr remove 251,803 + dinB b0231 0.86 +0.3 251,803 + dinB b0231 0.86 +0.1 251,818 + dinB b0231 0.87 +0.2 251,880 + +0.1 251,901 + +0.2 251,909 - -0.4 251,924 + +0.4 251,965 - +1.0 252,003 - +0.4 252,137 - yafN b0232 0.45 -0.3 252,155 - yafN b0232 0.51 -0.0 252,288 + +0.2 252,331 + +0.4 252,331 + +0.3 252,341 - yafO b0233 0.10 +2.0 252,346 + yafO b0233 0.11 +1.0 252,368 - yafO b0233 0.17 +1.0 252,368 - yafO b0233 0.17 +1.0 252,380 + yafO b0233 0.20 -0.1 252,381 + yafO b0233 0.20 +0.1 252,419 + yafO b0233 0.30 +2.2 252,427 + yafO b0233 0.32 +1.0 252,427 + yafO b0233 0.32 +1.0 252,440 + yafO b0233 0.35 +1.5 252,440 + yafO b0233 0.35 -0.8 252,440 + yafO b0233 0.35 -0.6 252,440 + yafO b0233 0.35 +0.1 252,440 + yafO b0233 0.35 -0.1 252,443 - yafO b0233 0.36 +1.0 252,475 + yafO b0233 0.44 -2.0 252,522 - yafO b0233 0.55 +0.2 252,522 - yafO b0233 0.55 +0.4 252,534 - yafO b0233 0.58 +1.0 252,538 + yafO b0233 0.59 -0.2 252,538 + yafO b0233 0.59 +1.2 252,563 + yafO b0233 0.66 -0.0 252,566 - yafO b0233 0.66 +1.0 252,574 - yafO b0233 0.68 -0.2 252,574 - yafO b0233 0.68 +0.8 252,592 - yafO b0233 0.73 +1.0 252,594 - yafO b0233 0.73 +1.0 252,597 + yafO b0233 0.74 +0.1 252,610 - yafO b0233 0.77 +1.0 252,614 + yafO b0233 0.78 +0.1 252,622 - yafO b0233 0.80 +1.0 252,628 + yafO b0233 0.82 -0.8 252,671 + +0.5 252,671 + -0.1 252,739 - +1.0 252,779 - yafP b0234 0.15 +1.0 252,781 + yafP b0234 0.16 +1.0 252,848 + yafP b0234 0.31 -1.0 252,849 + yafP b0234 0.31 +1.0 252,879 + yafP b0234 0.38 +1.0 252,896 + yafP b0234 0.41 +1.0 252,936 + yafP b0234 0.50 +0.3 252,973 + yafP b0234 0.58 +1.0 252,974 + yafP b0234 0.58 +1.0 253,041 + yafP b0234 0.73 +1.0 253,077 + yafP b0234 0.81 -0.6 253,087 + yafP b0234 0.83 -0.6 253,087 + yafP b0234 0.83 -2.0 253,087 + yafP b0234 0.83 -1.3 253,102 + yafP b0234 0.87 -1.0 253,188 + -0.0 253,198 - +1.0 253,220 + -0.0 253,222 + -0.0 253,222 - +1.0 253,272 - +1.0 253,275 + -0.4 253,283 - +1.0 253,301 + +1.0 253,350 - +0.1 253,350 - +0.3 253,350 - +0.2 253,350 - -0.1 253,376 - -0.1 253,381 - +3.3 253,383 - +0.2 253,386 + +1.0 253,387 + +1.0 253,394 + +1.0 253,394 + +1.0 253,396 - +0.1 253,422 - +0.4 253,422 - +1.3 253,423 - -0.4 253,423 - +0.7 253,427 - +0.8 253,428 - +0.1 253,430 + +0.4 253,430 + +1.4 253,430 + +0.1 253,430 + +0.5 253,432 - -0.3 253,432 - -0.8 253,434 + -0.0 253,437 - +0.2 253,438 - +2.0 253,438 - -0.6 253,438 - +0.5 253,438 - +1.6 253,450 - +0.8 253,454 - +1.6 253,491 + +2.6 253,504 - ykfJ b0235 0.12 -0.1 253,504 - ykfJ b0235 0.12 -0.4 253,520 - ykfJ b0235 0.20 +0.4 253,520 - ykfJ b0235 0.20 -0.2 253,520 - ykfJ b0235 0.20 -0.0 253,523 + ykfJ b0235 0.21 +1.0 253,533 - ykfJ b0235 0.26 -0.4 253,533 - ykfJ b0235 0.26 -0.9 253,533 - ykfJ b0235 0.26 +0.6 253,533 - ykfJ b0235 0.26 -0.1 253,535 + ykfJ b0235 0.27 +1.0 253,535 + ykfJ b0235 0.27 +1.0 253,543 - ykfJ b0235 0.31 +0.6 253,543 - ykfJ b0235 0.31 +0.2 253,543 - ykfJ b0235 0.31 +0.6 253,543 - ykfJ b0235 0.31 +0.3 253,543 - ykfJ b0235 0.31 +0.5 253,543 - ykfJ b0235 0.31 +0.1 253,543 - ykfJ b0235 0.31 +1.4 253,543 - ykfJ b0235 0.31 +0.5 253,557 - ykfJ b0235 0.38 +0.2 253,569 - ykfJ b0235 0.43 +0.3 253,571 - ykfJ b0235 0.44 +0.3 253,593 + ykfJ b0235 0.55 -0.0 253,603 + ykfJ b0235 0.60 +0.2 253,608 - ykfJ b0235 0.62 +0.6 253,617 + ykfJ b0235 0.67 +0.4 253,617 + ykfJ b0235 0.67 -0.1 253,617 + ykfJ b0235 0.67 +1.1 253,617 + ykfJ b0235 0.67 -0.0 253,625 - ykfJ b0235 0.71 +0.8 253,625 - ykfJ b0235 0.71 -0.3 253,625 - ykfJ b0235 0.71 +0.9 253,633 - ykfJ b0235 0.74 +0.4 253,637 - ykfJ b0235 0.76 +0.1 253,642 - ykfJ b0235 0.79 +0.4 253,642 - ykfJ b0235 0.79 +0.0 253,642 - ykfJ b0235 0.79 +0.5 253,681 + -0.3 253,727 + +0.4 253,728 + +0.5 253,728 + -0.5 253,728 + +0.3 253,734 - +0.6 253,734 - -1.1 253,735 - -0.4 253,735 - +0.2 253,736 - -0.5 253,736 - -0.5 253,736 - +0.1 253,747 + +0.4 253,765 + prfH b0236 0.13 -0.1 253,773 + prfH b0236 0.14 +0.5 253,805 - prfH b0236 0.21 +0.7 253,805 - prfH b0236 0.21 -0.3 253,838 - prfH b0236 0.27 +0.5 253,891 - prfH b0236 0.38 -0.7
Or see this region's nucleotide sequence