Experiment: LB Time9hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ksgA and pdxA overlap by 4 nucleotides pdxA and surA overlap by 1 nucleotides surA and imp are separated by 52 nucleotides
b0051: ksgA - dimethyladenosine transferase (NCBI), at 51,609 to 52,430
ksgA
b0052: pdxA - 4-hydroxythreonine-4-phosphate dehydrogenase (NCBI), at 52,427 to 53,416
pdxA
b0053: surA - peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI), at 53,416 to 54,702
surA
b0054: imp - organic solvent tolerance protein precursor (NCBI), at 54,755 to 57,109
imp
Position (kb) 53
54
55 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 52.420 kb on - strand at 52.490 kb on + strand at 52.490 kb on + strand at 52.502 kb on + strand at 52.502 kb on + strand at 52.518 kb on - strand at 52.518 kb on - strand at 52.626 kb on + strand, within pdxA at 52.688 kb on - strand, within pdxA at 52.735 kb on + strand, within pdxA at 52.735 kb on + strand, within pdxA at 52.740 kb on - strand, within pdxA at 52.766 kb on - strand, within pdxA at 52.780 kb on - strand, within pdxA at 52.797 kb on - strand, within pdxA at 52.879 kb on - strand, within pdxA at 52.914 kb on + strand, within pdxA at 52.982 kb on - strand, within pdxA at 52.982 kb on - strand, within pdxA at 52.983 kb on + strand, within pdxA at 53.061 kb on - strand, within pdxA at 53.062 kb on - strand, within pdxA at 53.062 kb on - strand at 53.064 kb on + strand, within pdxA at 53.067 kb on - strand, within pdxA at 53.163 kb on - strand, within pdxA at 53.163 kb on - strand, within pdxA at 53.181 kb on - strand, within pdxA at 53.181 kb on - strand, within pdxA at 53.202 kb on - strand, within pdxA at 53.221 kb on + strand, within pdxA at 53.221 kb on + strand, within pdxA at 53.226 kb on - strand, within pdxA at 53.246 kb on - strand, within pdxA at 53.291 kb on + strand, within pdxA at 53.328 kb on + strand at 53.328 kb on + strand at 53.338 kb on + strand at 53.338 kb on + strand at 53.338 kb on + strand at 53.338 kb on + strand at 53.346 kb on - strand at 53.346 kb on - strand at 53.346 kb on - strand at 53.460 kb on - strand at 53.460 kb on - strand at 53.468 kb on - strand at 53.524 kb on - strand at 53.552 kb on + strand, within surA at 53.554 kb on - strand, within surA at 53.565 kb on - strand, within surA at 53.565 kb on - strand, within surA at 53.591 kb on + strand, within surA at 53.603 kb on + strand, within surA at 53.616 kb on - strand, within surA at 53.633 kb on - strand, within surA at 53.633 kb on - strand, within surA at 53.667 kb on + strand, within surA at 53.695 kb on - strand, within surA at 53.702 kb on - strand, within surA at 53.702 kb on - strand, within surA at 53.704 kb on + strand, within surA at 53.736 kb on + strand, within surA at 53.766 kb on - strand, within surA at 53.766 kb on - strand, within surA at 53.776 kb on - strand, within surA at 53.797 kb on - strand, within surA at 53.802 kb on + strand, within surA at 53.807 kb on - strand, within surA at 53.910 kb on + strand, within surA at 53.991 kb on + strand, within surA at 53.997 kb on - strand, within surA at 54.015 kb on - strand, within surA at 54.015 kb on - strand, within surA at 54.015 kb on - strand, within surA at 54.035 kb on - strand, within surA at 54.048 kb on + strand, within surA at 54.148 kb on + strand, within surA at 54.166 kb on - strand, within surA at 54.166 kb on - strand, within surA at 54.178 kb on + strand, within surA at 54.196 kb on - strand, within surA at 54.205 kb on + strand, within surA at 54.285 kb on - strand, within surA at 54.285 kb on - strand, within surA at 54.406 kb on + strand, within surA at 54.487 kb on - strand, within surA at 54.495 kb on - strand, within surA at 54.507 kb on + strand, within surA at 54.568 kb on - strand, within surA at 54.612 kb on - strand at 54.612 kb on - strand at 54.612 kb on - strand at 54.637 kb on - strand at 54.826 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Time9hr remove 52,420 - +1.4 52,490 + -1.0 52,490 + -0.1 52,502 + -1.0 52,502 + -0.5 52,518 - -0.6 52,518 - +0.4 52,626 + pdxA b0052 0.20 -0.1 52,688 - pdxA b0052 0.26 +0.0 52,735 + pdxA b0052 0.31 -0.2 52,735 + pdxA b0052 0.31 -0.5 52,740 - pdxA b0052 0.32 +0.6 52,766 - pdxA b0052 0.34 +0.3 52,780 - pdxA b0052 0.36 +0.6 52,797 - pdxA b0052 0.37 +0.0 52,879 - pdxA b0052 0.46 -0.3 52,914 + pdxA b0052 0.49 +0.4 52,982 - pdxA b0052 0.56 -0.9 52,982 - pdxA b0052 0.56 -0.4 52,983 + pdxA b0052 0.56 -0.0 53,061 - pdxA b0052 0.64 +0.1 53,062 - pdxA b0052 0.64 +1.2 53,062 - -0.8 53,064 + pdxA b0052 0.64 +0.2 53,067 - pdxA b0052 0.65 -0.1 53,163 - pdxA b0052 0.74 -0.1 53,163 - pdxA b0052 0.74 -0.2 53,181 - pdxA b0052 0.76 +0.1 53,181 - pdxA b0052 0.76 +0.2 53,202 - pdxA b0052 0.78 +0.8 53,221 + pdxA b0052 0.80 +0.1 53,221 + pdxA b0052 0.80 +0.4 53,226 - pdxA b0052 0.81 +0.1 53,246 - pdxA b0052 0.83 -0.2 53,291 + pdxA b0052 0.87 -0.1 53,328 + -0.6 53,328 + +0.6 53,338 + +0.6 53,338 + +0.0 53,338 + -0.4 53,338 + -0.1 53,346 - -0.4 53,346 - +0.9 53,346 - -0.1 53,460 - +0.0 53,460 - -0.2 53,468 - -0.5 53,524 - -0.5 53,552 + surA b0053 0.11 -0.9 53,554 - surA b0053 0.11 -0.4 53,565 - surA b0053 0.12 +0.0 53,565 - surA b0053 0.12 +0.2 53,591 + surA b0053 0.14 -0.5 53,603 + surA b0053 0.15 -1.8 53,616 - surA b0053 0.16 -0.3 53,633 - surA b0053 0.17 -0.8 53,633 - surA b0053 0.17 -1.3 53,667 + surA b0053 0.20 +0.6 53,695 - surA b0053 0.22 -0.8 53,702 - surA b0053 0.22 -1.2 53,702 - surA b0053 0.22 -2.0 53,704 + surA b0053 0.22 -1.0 53,736 + surA b0053 0.25 +0.0 53,766 - surA b0053 0.27 +0.1 53,766 - surA b0053 0.27 -0.4 53,776 - surA b0053 0.28 -0.5 53,797 - surA b0053 0.30 +0.6 53,802 + surA b0053 0.30 -0.3 53,807 - surA b0053 0.30 -1.1 53,910 + surA b0053 0.38 +0.0 53,991 + surA b0053 0.45 +0.0 53,997 - surA b0053 0.45 -0.3 54,015 - surA b0053 0.47 -1.1 54,015 - surA b0053 0.47 +1.0 54,015 - surA b0053 0.47 -0.8 54,035 - surA b0053 0.48 -0.7 54,048 + surA b0053 0.49 -0.4 54,148 + surA b0053 0.57 -1.5 54,166 - surA b0053 0.58 +2.4 54,166 - surA b0053 0.58 +0.0 54,178 + surA b0053 0.59 -1.2 54,196 - surA b0053 0.61 -0.1 54,205 + surA b0053 0.61 -1.1 54,285 - surA b0053 0.68 +1.0 54,285 - surA b0053 0.68 -1.5 54,406 + surA b0053 0.77 -0.5 54,487 - surA b0053 0.83 +0.5 54,495 - surA b0053 0.84 -2.4 54,507 + surA b0053 0.85 +0.0 54,568 - surA b0053 0.90 +0.0 54,612 - -0.3 54,612 - -0.3 54,612 - -0.1 54,637 - +3.9 54,826 - -0.2
Or see this region's nucleotide sequence