Experiment: P. put PUT 10 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4489 and PP_4490 are separated by 257 nucleotides PP_4490 and PP_4491 are separated by 39 nucleotides PP_4491 and PP_5694 are separated by 189 nucleotides
PP_4489: PP_4489 - sigma-54 dependent transcriptional regulator, at 5,100,067 to 5,101,626
_4489
PP_4490: PP_4490 - phenylalanine-4-hydroxylase, at 5,101,884 to 5,102,672
_4490
PP_4491: PP_4491 - pterin-4-alpha-carbinolamine dehydratase, at 5,102,712 to 5,103,068
_4491
PP_5694: PP_5694 - conserved protein of unknown function, at 5,103,258 to 5,103,386
_5694
Position (kb) 5101
5102
5103 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5101.046 kb on + strand, within PP_4489 at 5101.046 kb on + strand, within PP_4489 at 5101.046 kb on + strand, within PP_4489 at 5101.046 kb on + strand, within PP_4489 at 5101.046 kb on + strand, within PP_4489 at 5101.046 kb on + strand, within PP_4489 at 5101.046 kb on + strand, within PP_4489 at 5101.047 kb on - strand, within PP_4489 at 5101.047 kb on - strand, within PP_4489 at 5101.047 kb on - strand, within PP_4489 at 5101.047 kb on - strand, within PP_4489 at 5101.050 kb on + strand, within PP_4489 at 5101.050 kb on + strand, within PP_4489 at 5101.051 kb on - strand, within PP_4489 at 5101.234 kb on + strand, within PP_4489 at 5101.235 kb on - strand, within PP_4489 at 5101.235 kb on - strand, within PP_4489 at 5101.238 kb on + strand, within PP_4489 at 5101.238 kb on + strand, within PP_4489 at 5101.419 kb on + strand, within PP_4489 at 5101.502 kb on + strand at 5101.502 kb on + strand at 5101.503 kb on - strand at 5101.543 kb on + strand at 5101.544 kb on - strand at 5101.544 kb on - strand at 5101.620 kb on + strand at 5101.681 kb on + strand at 5101.681 kb on + strand at 5101.828 kb on + strand at 5101.829 kb on - strand at 5101.853 kb on + strand at 5101.854 kb on - strand at 5101.854 kb on - strand at 5101.854 kb on - strand at 5101.854 kb on - strand at 5101.854 kb on - strand at 5101.899 kb on + strand at 5101.899 kb on + strand at 5101.900 kb on - strand at 5101.900 kb on - strand at 5101.900 kb on - strand at 5101.900 kb on - strand at 5101.931 kb on + strand at 5101.932 kb on - strand at 5102.019 kb on + strand, within PP_4490 at 5102.221 kb on - strand, within PP_4490 at 5102.308 kb on - strand, within PP_4490 at 5102.350 kb on - strand, within PP_4490 at 5102.364 kb on + strand, within PP_4490 at 5102.419 kb on - strand, within PP_4490 at 5102.419 kb on - strand, within PP_4490 at 5102.457 kb on + strand, within PP_4490 at 5102.457 kb on + strand, within PP_4490 at 5102.458 kb on - strand, within PP_4490 at 5102.458 kb on - strand, within PP_4490 at 5102.513 kb on + strand, within PP_4490 at 5102.513 kb on + strand, within PP_4490 at 5102.514 kb on - strand, within PP_4490 at 5102.514 kb on - strand, within PP_4490 at 5102.514 kb on - strand, within PP_4490 at 5102.514 kb on - strand, within PP_4490 at 5102.548 kb on - strand, within PP_4490 at 5102.555 kb on - strand, within PP_4490 at 5102.615 kb on + strand at 5102.615 kb on + strand at 5102.615 kb on + strand at 5102.825 kb on - strand, within PP_4491 at 5103.066 kb on + strand at 5103.066 kb on + strand at 5103.067 kb on - strand at 5103.112 kb on + strand at 5103.112 kb on + strand at 5103.112 kb on + strand at 5103.112 kb on + strand at 5103.113 kb on - strand at 5103.113 kb on - strand at 5103.113 kb on - strand at 5103.113 kb on - strand at 5103.113 kb on - strand at 5103.384 kb on + strand at 5103.384 kb on + strand at 5103.384 kb on + strand at 5103.384 kb on + strand at 5103.385 kb on - strand at 5103.385 kb on - strand at 5103.458 kb on - strand at 5103.471 kb on + strand at 5103.472 kb on - strand at 5103.472 kb on - strand at 5103.472 kb on - strand at 5103.472 kb on - strand at 5103.472 kb on - strand at 5103.634 kb on + strand at 5103.634 kb on + strand at 5103.635 kb on - strand at 5103.635 kb on - strand at 5103.635 kb on - strand at 5103.635 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. put PUT 10 MOI 0.1 remove 5,101,046 + PP_4489 0.63 -2.3 5,101,046 + PP_4489 0.63 +0.6 5,101,046 + PP_4489 0.63 -3.5 5,101,046 + PP_4489 0.63 -0.5 5,101,046 + PP_4489 0.63 -1.1 5,101,046 + PP_4489 0.63 +0.1 5,101,046 + PP_4489 0.63 -1.0 5,101,047 - PP_4489 0.63 -1.0 5,101,047 - PP_4489 0.63 -1.1 5,101,047 - PP_4489 0.63 -2.1 5,101,047 - PP_4489 0.63 +0.6 5,101,050 + PP_4489 0.63 -1.0 5,101,050 + PP_4489 0.63 +0.9 5,101,051 - PP_4489 0.63 -0.6 5,101,234 + PP_4489 0.75 -1.4 5,101,235 - PP_4489 0.75 -0.6 5,101,235 - PP_4489 0.75 -2.4 5,101,238 + PP_4489 0.75 -0.4 5,101,238 + PP_4489 0.75 -0.6 5,101,419 + PP_4489 0.87 +0.1 5,101,502 + -0.5 5,101,502 + +0.6 5,101,503 - -1.0 5,101,543 + -0.3 5,101,544 - -0.5 5,101,544 - -0.2 5,101,620 + -1.4 5,101,681 + +0.3 5,101,681 + -0.1 5,101,828 + -0.9 5,101,829 - -1.0 5,101,853 + -1.1 5,101,854 - -0.6 5,101,854 - -0.8 5,101,854 - -0.4 5,101,854 - -0.7 5,101,854 - -0.3 5,101,899 + -0.5 5,101,899 + -2.3 5,101,900 - -1.0 5,101,900 - -0.5 5,101,900 - -0.8 5,101,900 - -0.7 5,101,931 + -1.1 5,101,932 - -2.4 5,102,019 + PP_4490 0.17 -1.2 5,102,221 - PP_4490 0.43 -0.6 5,102,308 - PP_4490 0.54 -1.2 5,102,350 - PP_4490 0.59 -1.5 5,102,364 + PP_4490 0.61 -1.0 5,102,419 - PP_4490 0.68 -3.3 5,102,419 - PP_4490 0.68 -0.6 5,102,457 + PP_4490 0.73 -0.2 5,102,457 + PP_4490 0.73 -1.4 5,102,458 - PP_4490 0.73 +0.2 5,102,458 - PP_4490 0.73 +0.6 5,102,513 + PP_4490 0.80 +0.0 5,102,513 + PP_4490 0.80 -0.6 5,102,514 - PP_4490 0.80 +0.0 5,102,514 - PP_4490 0.80 -1.1 5,102,514 - PP_4490 0.80 +0.4 5,102,514 - PP_4490 0.80 -0.7 5,102,548 - PP_4490 0.84 -0.5 5,102,555 - PP_4490 0.85 +1.0 5,102,615 + +0.3 5,102,615 + -0.1 5,102,615 + -1.4 5,102,825 - PP_4491 0.32 -0.1 5,103,066 + +0.5 5,103,066 + +0.3 5,103,067 - +0.5 5,103,112 + -0.7 5,103,112 + -0.4 5,103,112 + -0.6 5,103,112 + +3.6 5,103,113 - +0.3 5,103,113 - +0.4 5,103,113 - -0.3 5,103,113 - +0.8 5,103,113 - -0.4 5,103,384 + -0.5 5,103,384 + -0.1 5,103,384 + +0.3 5,103,384 + -0.3 5,103,385 - -0.8 5,103,385 - -0.1 5,103,458 - -1.0 5,103,471 + -0.2 5,103,472 - +0.5 5,103,472 - +0.3 5,103,472 - -0.4 5,103,472 - +0.3 5,103,472 - -0.2 5,103,634 + +0.2 5,103,634 + +0.9 5,103,635 - -0.9 5,103,635 - -0.6 5,103,635 - +0.7 5,103,635 - +0.1
Or see this region's nucleotide sequence