Strain Fitness in Pseudomonas putida KT2440 around PP_2144

Experiment: P. put PUT 10 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_2142 and PP_2143 are separated by 13 nucleotidesPP_2143 and PP_2144 are separated by 215 nucleotidesPP_2144 and PP_2145 are separated by 177 nucleotides PP_2142: PP_2142 - Cell division inhibitor SulA, at 2,444,413 to 2,444,889 _2142 PP_2143: PP_2143 - transcriptional repressor, at 2,444,903 to 2,445,511 _2143 PP_2144: PP_2144 - Transcriptional regulator, TetR family, at 2,445,727 to 2,446,434 _2144 PP_2145: PP_2145 - beta-N-acetylglucosaminidase, at 2,446,612 to 2,447,622 _2145 Position (kb) 2445 2446 2447Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2445.156 kb on - strand, within PP_2143at 2445.169 kb on - strand, within PP_2143at 2445.549 kb on - strandat 2445.682 kb on + strandat 2445.713 kb on + strandat 2445.723 kb on - strandat 2445.819 kb on + strand, within PP_2144at 2445.819 kb on + strand, within PP_2144at 2446.005 kb on - strand, within PP_2144at 2446.005 kb on - strand, within PP_2144at 2446.109 kb on - strand, within PP_2144at 2446.123 kb on + strand, within PP_2144at 2446.123 kb on + strand, within PP_2144at 2446.123 kb on + strandat 2446.124 kb on - strand, within PP_2144at 2446.124 kb on - strand, within PP_2144at 2446.124 kb on - strand, within PP_2144at 2446.124 kb on - strand, within PP_2144at 2446.124 kb on - strand, within PP_2144at 2446.144 kb on + strand, within PP_2144at 2446.523 kb on + strandat 2446.524 kb on - strandat 2446.524 kb on - strandat 2446.657 kb on - strandat 2446.657 kb on - strandat 2446.732 kb on - strand, within PP_2145at 2446.826 kb on + strand, within PP_2145at 2446.827 kb on - strand, within PP_2145at 2447.257 kb on + strand, within PP_2145at 2447.258 kb on - strand, within PP_2145at 2447.258 kb on - strand, within PP_2145at 2447.258 kb on - strand, within PP_2145at 2447.258 kb on - strand, within PP_2145at 2447.434 kb on - strand, within PP_2145

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Per-strain Table

Position Strand Gene LocusTag Fraction P. put PUT 10 MOI 0.1
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2,445,156 - PP_2143 0.42 -1.3
2,445,169 - PP_2143 0.44 +1.6
2,445,549 - -0.9
2,445,682 + +0.5
2,445,713 + +0.8
2,445,723 - +0.9
2,445,819 + PP_2144 0.13 +1.3
2,445,819 + PP_2144 0.13 +0.1
2,446,005 - PP_2144 0.39 -1.0
2,446,005 - PP_2144 0.39 +0.9
2,446,109 - PP_2144 0.54 +0.7
2,446,123 + PP_2144 0.56 +0.5
2,446,123 + PP_2144 0.56 -0.5
2,446,123 + +0.6
2,446,124 - PP_2144 0.56 +0.2
2,446,124 - PP_2144 0.56 +2.1
2,446,124 - PP_2144 0.56 +1.2
2,446,124 - PP_2144 0.56 +0.9
2,446,124 - PP_2144 0.56 -1.3
2,446,144 + PP_2144 0.59 +0.9
2,446,523 + +3.2
2,446,524 - -2.2
2,446,524 - -2.4
2,446,657 - -1.6
2,446,657 - -1.5
2,446,732 - PP_2145 0.12 +0.6
2,446,826 + PP_2145 0.21 -2.2
2,446,827 - PP_2145 0.21 -1.2
2,447,257 + PP_2145 0.64 -0.4
2,447,258 - PP_2145 0.64 -2.4
2,447,258 - PP_2145 0.64 -1.7
2,447,258 - PP_2145 0.64 -1.0
2,447,258 - PP_2145 0.64 +0.0
2,447,434 - PP_2145 0.81 -2.6

Or see this region's nucleotide sequence