Experiment: P. put PUT 10 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0186 and PP_0187 overlap by 4 nucleotides PP_0187 and PP_0188 are separated by 18 nucleotides
PP_0186: PP_0186 - Porphobilinogen deaminase, at 237,982 to 238,923
_0186
PP_0187: PP_0187 - Uroporphyrinogen-III synthase, at 238,920 to 239,687
_0187
PP_0188: PP_0188 - putative Uroporphyrin-III C-methyltransferase, at 239,706 to 240,833
_0188
Position (kb) 238
239
240 Strain fitness (log2 ratio) -1
0
1
2
3 at 239.685 kb on + strand at 239.685 kb on + strand at 239.685 kb on + strand at 239.685 kb on + strand at 239.685 kb on + strand at 239.686 kb on - strand at 239.693 kb on + strand at 239.694 kb on - strand at 239.694 kb on - strand at 239.730 kb on + strand at 239.730 kb on + strand at 239.731 kb on - strand at 239.731 kb on - strand at 239.731 kb on - strand at 239.762 kb on + strand at 239.763 kb on - strand at 239.763 kb on - strand at 239.763 kb on - strand at 239.872 kb on + strand, within PP_0188 at 239.872 kb on + strand, within PP_0188 at 239.873 kb on - strand, within PP_0188 at 240.028 kb on + strand, within PP_0188 at 240.161 kb on + strand, within PP_0188 at 240.323 kb on + strand, within PP_0188 at 240.324 kb on - strand, within PP_0188 at 240.365 kb on - strand, within PP_0188 at 240.402 kb on - strand, within PP_0188 at 240.475 kb on + strand, within PP_0188 at 240.483 kb on + strand, within PP_0188 at 240.484 kb on - strand, within PP_0188 at 240.575 kb on + strand, within PP_0188 at 240.576 kb on - strand, within PP_0188 at 240.652 kb on + strand, within PP_0188 at 240.653 kb on - strand, within PP_0188
Per-strain Table
Position Strand Gene LocusTag Fraction P. put PUT 10 MOI 0.1 remove 239,685 + -0.1 239,685 + -0.1 239,685 + -0.7 239,685 + -0.4 239,685 + -1.1 239,686 - -0.0 239,693 + +0.3 239,694 - -1.2 239,694 - +1.0 239,730 + +0.1 239,730 + +0.7 239,731 - +0.8 239,731 - -0.5 239,731 - +2.4 239,762 + +0.0 239,763 - -0.8 239,763 - +0.6 239,763 - +0.2 239,872 + PP_0188 0.15 -1.6 239,872 + PP_0188 0.15 +0.0 239,873 - PP_0188 0.15 +0.2 240,028 + PP_0188 0.29 -1.5 240,161 + PP_0188 0.40 +0.0 240,323 + PP_0188 0.55 -1.2 240,324 - PP_0188 0.55 -0.6 240,365 - PP_0188 0.58 -0.1 240,402 - PP_0188 0.62 +1.2 240,475 + PP_0188 0.68 -0.0 240,483 + PP_0188 0.69 +0.2 240,484 - PP_0188 0.69 +0.2 240,575 + PP_0188 0.77 +0.6 240,576 - PP_0188 0.77 +2.8 240,652 + PP_0188 0.84 +1.5 240,653 - PP_0188 0.84 -0.6
Or see this region's nucleotide sequence