Experiment: P. put PUT 10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4935 and PP_4936 are separated by 15 nucleotides PP_4936 and PP_4937 are separated by 100 nucleotides PP_4937 and PP_4938 are separated by 3 nucleotides
PP_4935: PP_4935 - Lipid A export ATP-binding/permease protein MsbA, at 5,615,652 to 5,617,460
_4935
PP_4936: PP_4936 - O-antigen polymerase, at 5,617,476 to 5,618,651
_4936
PP_4937: PP_4937 - toluene tolerance protein, at 5,618,752 to 5,619,357
_4937
PP_4938: PP_4938 - putative Glycosyl transferase, at 5,619,361 to 5,620,500
_4938
Position (kb) 5617
5618
5619 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 5617.586 kb on + strand at 5617.810 kb on + strand, within PP_4936 at 5617.810 kb on + strand, within PP_4936 at 5617.918 kb on + strand, within PP_4936 at 5617.919 kb on - strand, within PP_4936 at 5617.919 kb on - strand, within PP_4936 at 5618.015 kb on + strand, within PP_4936 at 5618.016 kb on - strand, within PP_4936 at 5618.129 kb on - strand, within PP_4936 at 5618.161 kb on + strand, within PP_4936 at 5618.161 kb on + strand, within PP_4936 at 5618.161 kb on + strand, within PP_4936 at 5618.161 kb on + strand, within PP_4936 at 5618.161 kb on + strand, within PP_4936 at 5618.162 kb on - strand, within PP_4936 at 5618.162 kb on - strand, within PP_4936 at 5618.162 kb on - strand, within PP_4936 at 5618.162 kb on - strand, within PP_4936 at 5618.162 kb on - strand, within PP_4936 at 5618.162 kb on - strand, within PP_4936 at 5618.184 kb on + strand, within PP_4936 at 5618.295 kb on + strand, within PP_4936 at 5618.295 kb on + strand, within PP_4936 at 5618.296 kb on - strand, within PP_4936 at 5618.363 kb on - strand, within PP_4936 at 5618.363 kb on - strand, within PP_4936 at 5618.363 kb on - strand, within PP_4936 at 5618.514 kb on + strand, within PP_4936 at 5618.515 kb on - strand, within PP_4936 at 5618.616 kb on + strand at 5618.617 kb on - strand at 5618.644 kb on + strand at 5618.645 kb on - strand at 5618.690 kb on + strand at 5618.690 kb on + strand at 5618.690 kb on + strand at 5618.691 kb on - strand at 5618.691 kb on - strand at 5618.698 kb on + strand at 5618.710 kb on + strand at 5618.711 kb on - strand at 5618.711 kb on - strand at 5618.747 kb on + strand at 5618.748 kb on - strand at 5618.748 kb on - strand at 5618.843 kb on - strand, within PP_4937 at 5618.909 kb on + strand, within PP_4937 at 5618.910 kb on - strand, within PP_4937 at 5618.917 kb on + strand, within PP_4937 at 5619.028 kb on + strand, within PP_4937 at 5619.028 kb on + strand, within PP_4937 at 5619.029 kb on - strand, within PP_4937 at 5619.094 kb on + strand, within PP_4937 at 5619.095 kb on - strand, within PP_4937 at 5619.095 kb on - strand, within PP_4937 at 5619.286 kb on + strand, within PP_4937 at 5619.287 kb on - strand, within PP_4937 at 5619.287 kb on - strand, within PP_4937 at 5619.287 kb on - strand, within PP_4937 at 5619.469 kb on - strand at 5619.469 kb on - strand at 5619.519 kb on - strand, within PP_4938 at 5619.577 kb on + strand, within PP_4938 at 5619.634 kb on + strand at 5619.635 kb on - strand, within PP_4938 at 5619.640 kb on + strand, within PP_4938 at 5619.641 kb on - strand, within PP_4938
Per-strain Table
Position Strand Gene LocusTag Fraction P. put PUT 10 MOI 1 remove 5,617,586 + -3.1 5,617,810 + PP_4936 0.28 -3.4 5,617,810 + PP_4936 0.28 -4.1 5,617,918 + PP_4936 0.38 -2.2 5,617,919 - PP_4936 0.38 -3.0 5,617,919 - PP_4936 0.38 -3.3 5,618,015 + PP_4936 0.46 -3.7 5,618,016 - PP_4936 0.46 -1.4 5,618,129 - PP_4936 0.56 -4.8 5,618,161 + PP_4936 0.58 -3.4 5,618,161 + PP_4936 0.58 -4.6 5,618,161 + PP_4936 0.58 -5.2 5,618,161 + PP_4936 0.58 -2.5 5,618,161 + PP_4936 0.58 -3.1 5,618,162 - PP_4936 0.58 -4.0 5,618,162 - PP_4936 0.58 -2.8 5,618,162 - PP_4936 0.58 -3.5 5,618,162 - PP_4936 0.58 -3.5 5,618,162 - PP_4936 0.58 -4.0 5,618,162 - PP_4936 0.58 -3.3 5,618,184 + PP_4936 0.60 -3.6 5,618,295 + PP_4936 0.70 -3.5 5,618,295 + PP_4936 0.70 -3.0 5,618,296 - PP_4936 0.70 -2.2 5,618,363 - PP_4936 0.75 -2.5 5,618,363 - PP_4936 0.75 -2.5 5,618,363 - PP_4936 0.75 -3.5 5,618,514 + PP_4936 0.88 -0.7 5,618,515 - PP_4936 0.88 -3.4 5,618,616 + -2.7 5,618,617 - -4.5 5,618,644 + -4.5 5,618,645 - -5.5 5,618,690 + -4.3 5,618,690 + -3.7 5,618,690 + -4.5 5,618,691 - +1.6 5,618,691 - +1.7 5,618,698 + -5.3 5,618,710 + -3.3 5,618,711 - +1.7 5,618,711 - +1.9 5,618,747 + -2.4 5,618,748 - +3.3 5,618,748 - +0.8 5,618,843 - PP_4937 0.15 +1.2 5,618,909 + PP_4937 0.26 -3.4 5,618,910 - PP_4937 0.26 +1.7 5,618,917 + PP_4937 0.27 -3.0 5,619,028 + PP_4937 0.46 -3.9 5,619,028 + PP_4937 0.46 -3.5 5,619,029 - PP_4937 0.46 +2.2 5,619,094 + PP_4937 0.56 -3.0 5,619,095 - PP_4937 0.57 +1.9 5,619,095 - PP_4937 0.57 +1.3 5,619,286 + PP_4937 0.88 -2.1 5,619,287 - PP_4937 0.88 +1.6 5,619,287 - PP_4937 0.88 +2.1 5,619,287 - PP_4937 0.88 +1.9 5,619,469 - -3.2 5,619,469 - -2.6 5,619,519 - PP_4938 0.14 -3.0 5,619,577 + PP_4938 0.19 -5.1 5,619,634 + +1.6 5,619,635 - PP_4938 0.24 -2.2 5,619,640 + PP_4938 0.24 -3.6 5,619,641 - PP_4938 0.25 -4.4
Or see this region's nucleotide sequence